Figure 2 | Scientific Reports

Figure 2

From: Progressive Changes in CXCR4 Expression That Define Thymocyte Positive Selection Are Dispensable For Both Innate and Conventional αβT-cell Development

Figure 2

Positive Selection Initiation Involves Progressive CXCR4 Downregulation In DP Thymocytes. (a) Identification of CD69 DP, CD69+ DP, CD69+CD62L, and CD69CD62L+ SP4 and SP8 thymocytes. (b) Representative FACS analysis and MFI of CXCR4 and CCR9 expression in the indicated thymocyte subsets isolated from adult WT mice. (c) Representative FACS analysis and MFI of CXCR4 and CCR9 expression by Nur77GFP+ and Nur77GFP DP thymocytes from Nur77GFP mice. (d) Representative FACS analysis and MFI of CXCR4 expression by splenic T cell subsets in WT Rag2GFP mice, RTE (Recent Thymic Emigrants, Rag2GFP+), Naïve T cells (CD62L+CD44), Memory T cells (CD62LCD44+). Grey histograms in b and d show isotype control staining for CXCR4 or CCR9, pre-gated on CD69 DP thymocytes. Graphs in d show MFI of CXCR4 on CD62L+ SP thymocytes and CD69 DP thymocytes for comparison. MFI analysis is calculated from one experiment, representative of at least 3 independent experiments. n > 10, unpaired Student’s t test *p < 0.05, **p < 0.01***p < 0.001, ****p < 0.0001. Error bars represent SEM.