Figure 1 | Scientific Reports

Figure 1

From: Progressive Changes in CXCR4 Expression That Define Thymocyte Positive Selection Are Dispensable For Both Innate and Conventional αβT-cell Development

Figure 1

CXCL12 Is Expressed Throughout The Thymic Cortex. Confocal images showing (a) expression of CXCL12dsRed in the thymus, scale bar represents 500 μm (b) reticular morphology of CXCL12dsRed expressing cells, scale bar represents 50 μm (c) co-localisation of CXCL12dsRed+ cells and CD205+ cTEC, (d) absence of CXCL12dsRed expression by K5+ mTEC, (e) restriction of CXCL12dsRed+ cells to areas of the thymus containing CD4+CD8+ DP thymocytes. ‘C’ denotes cortex and ‘M’ denotes medulla, confocal images are representative of at least three mice. Scale bars in b-d represent 100 μm. (f) Representative FACS analysis and quantitation of CXCL12dsRed expression by UEA-1+EpCAM-1+ mTEC and Ly51+EpCAM-1+ cTEC, CD31+ endothelium and podoplanin+ mesenchyme. n > 5, unpaired Student’s t test ****p < 0.0001. (g) Purified thymic stromal cells from WT mice were analyzed by qPCR for Cxcl12 mRNA, normalized to β-actin (mean ± SEM). qPCR data is representative of at least two independent biological experiments. Error bars represent SEM.