Effect of nopal consumption on serum leptin and expression of genes of inflammation and oxidative stress in adipose tissue. Panel (A) and (B) show the concentration of fasting serum leptin after the pretreatment and treatment periods respectively. Relative mRNA abundance of (C) leptin, (D) Tnf-α, (E) NADPH oxidase (Nox) and (F) amyloid precursor protein (App) in adipose tissue of Wistar rats fed one of the following diets: control diet (C), high fat diet with 5% sucrose in drinking water (HFS), HFS + 5% nopal (HFS + N), and obese rats switched to C diet with (HFS-C + N) or without (HFS-C) 5% nopal. Data are expressed as mean ± SEM (n = 5/group). Comparisons among groups were analysed by 2-way ANOVA followed by Fisher’s PLSD test. Different letters indicate significant differences among groups (a > b > c > d), P < 0.05. Comparisons between two groups were analysed by student t-test.