Nopal consumption decreases metabolic endotoxemia, prevents the hypersecretion of glucose insulinotropic peptide (GIP) and improves glucose tolerance. Fasting serum lipopolysaccharide (LPS) after the pretreatment (A) and treatments (B) periods. Serum GIP after the pretreatment (C) and treatments (D) periods. ipGTT and area under curve (AUC) after the pretreatment (E) and treatments (F) periods. Wistar rats were fed one of the following diets: control diet (C), high fat diet with 5% sucrose in their drinking water (HFS), HFS + 5% nopal (HFS + N), and obese rats switched to C diet with (HFS-C + N) or without (HFS-C) 5% nopal. Data are expressed as mean ± SEM (n = 5/group). Comparisons among groups were analysed by 2-way ANOVA followed by Fisher’s PLSD test. Different letters indicate significant differences among groups (a > b > c > d). P < 0.05. Comparisons between two groups were analysed by student t test.