Effects of a barban or mancozeb challenge on transgenic Arabidopsis lines expressing the yeast Tpo1p or Pdr5p transporters. (a) Representative images of 7-d old seedlings of the wild type (Col-0) and TPO1 (TPO1L1, L2, L4) or PDR5 (PDR5L1, L5) transgenic lines grown on media containing barban (upper panel) or mancozeb (lower panel). Plant growth under control conditions is shown in Fig. 1 and Table 1. (b) Effect of barban or mancozeb toxicity on PR elongation (upper panel) and shoot biomass (lower panel) of seedlings of the wild type (Col-0) and TPO1 (TPO1L1, L2, L4) or PDR5 (PDR5L1, L5) transgenic lines. Values represent means ± SD (n = 8), with similar results being obtained in three independent experiments performed with different seed batches. Asterisks denote statistically significant differences between ScTPO1- or ScPDR5-expressing lines and the wild type (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t-test).