Figure 3 | Scientific Reports

Figure 3

From: Heterologous expression of the yeast Tpo1p or Pdr5p membrane transporters in Arabidopsis confers plant xenobiotic tolerance

Figure 3

Effects of a 2,4-D challenge on transgenic Arabidopsis lines expressing the yeast Tpo1p or Pdr5p transporters. (a) Representative images of 7-d old seedlings of the wild type (Col-0) and TPO1 (TPO1L1, L2, L4, L6) or PDR5 (PDR5L1, L3, L4, L5) transgenic lines grown on medium containing 2,4-D. Plant growth under control conditions is shown in Fig. 1 and Table 1. (b) Effect of 2,4-D toxicity on PR elongation (upper panel) and shoot biomass (lower panel) of seedlings of the wild type (Col-0) and TPO1 (TPO1L1, L2, L4, L6) or PDR5 (PDR5L1, L3, L4, L5) transgenic lines. (c) Accumulation of 14C-2,4-D in root tips from 5-d old seedlings of the wild type (Col-0) and TPO1 (TPO1L1, L2, L4) or PDR5 (PDR5L1, L5) transgenic lines. Values represent means ± SD (n = 8), with similar results being obtained in three independent experiments performed with different seed batches. Asterisks denote statistically significant differences between ScTPO1- or ScPDR5-expressing lines and the wild type (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t-test).