Subcellular localisation of the yeast Tpo1p and Pdr5p transporters in plant cells. (a–f) Confocal laser scanning microscopy images of Arabidopsis mesophyll protoplasts (a,d) or transgenic root tips (b,c,e,f), transiently or stably expressing either YFP alone (a,b,c) or the ScTPO1-YFP fusion (d,e,f), respectively, under the control of the 35S promoter. Root epidermal cell close-ups (c,f) are shown. The YFP and chloroplast autofluorescence signals are visualized by green and red coloration, respectively. (g–v) Confocal laser scanning microscopy images of individual tobacco leaf epidermal cells transiently expressing the ScTPO1-GFP (g,i,j,o,q,r) or ScPDR5-GFP (k,m,n,s,u,v) fusions and/or the tonoplast marker Ɣ-TIPmCherry (h,i,j,l,m,n) or the plasma-membrane marker PIP2A-mCherry (p,q,r,t,u,v) under the control of the 35S promoter. Merged images of whole-cell views (i,m,q,u) or nucleus close-ups (j,n,r,v) are shown. Arrowheads point to transvacuolar strands, and asterisks indicate fluorescence signals approaching the nucleus only on the side facing the exterior of the cell (o–v) or surrounding the nucleus (f). The GFP and mCherry signals are visualized by green and red coloration, respectively.