Figure 1 | Scientific Reports

Figure 1

From: Heterologous expression of the yeast Tpo1p or Pdr5p membrane transporters in Arabidopsis confers plant xenobiotic tolerance

Figure 1

Transgene expression and phenotype under control conditions of transgenic Arabidopsis seedlings expressing the yeast Tpo1p or Pdr5p transporters. (a) Standard RT-PCR (upper panels) and real-time RT-PCR (lower panels) analysis of S. cerevisiae TPO1 (left) and PDR5 (right) transcript levels in 7-d old seedlings of the wild type (Col-0) and TPO1 (TPO1L1-L6) or PDR5 (PDR5L1-L5) transgenic lines. Expression of the UBQ10 gene was used as a loading control or as a reference gene for standard RT-PCR and real-time RT-PCR, respectively. Values represent means ± SE (n = 4), with similar results being obtained in two independent experiments. (b) Representative images of 7-d old seedlings of the wild type (Col-0) and TPO1 (TPO1L1-L6) or PDR5 (PDR5L1-L5) transgenic lines grown under control conditions.