Functional neutralization of chemokine activity. (A) Neutralization of CCL2 induced THP-1 cell migration by P991_AMBCA. Y-axis shows cell count of THP-1 cells migrating through to the bottom chamber in response to EC80 dose of CCL2. Data (3 technical replicates) are shown as mean ± s.e.m. X-axis shows P991_AMBCA concentration (Log10 Molar). (B) Summary of P991_AMBCA IC50 data (moles/litre, mean, s.e.m. of 3 biological replicates analyzed as above) for chemokines that induce THP-1 cell migration. Lower IC50 is indicated as shades of green, medium as yellow, and high as shades of orange. (C) Neutralization of a complex chemokine mixture by P991_AMBCA. Y-axis shows AUC (area under progress curve) in response to indicated treatments shown on the x-axis. NIL indicates baseline migration without any chemokine. Results (mean ± s.e.m., and individual data points) of 3 independent biological replicate experiments are shown, and were analyzed with one-way ANOVA with Sidak’s multiple comparisons test and P values adjusted for multiple comparisons. *Indicates P < 0.05, **Indicates P < 0.01, ***P < 0.001, ****P < 0.0001.