Figure 5 | Scientific Reports

Figure 5

From: Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities

Figure 5

Binding of novel evasins to human CC chemokines. (A) Binding affinities (K d, moles/litre) of novel evasins to human CC-chemokines using biolayer interferometry. Higher affinity binding is indicated as shades of green, medium affinity as yellow, and lower affinity as shades of orange. Evasins and chemokines are arranged by sequence-similarity based phylogeny. - Indicates that binding was not detected at 300 nM chemokine concentration. (B) Dissociative half-life times (t1/2, minutes) of novel evasins with human CC-chemokines, calculated from biolayer interferometry off-rates (t1/2 = 0.693/(k off × 60)60. - Indicates that binding was not detected at 300 nM chemokine concentration. *Indicates that k off data were not available (as a steady-state fit was performed, see methods) and the dissociative half-life time could not be calculated. Evasins and chemokines are arranged by sequence-similarity based phylogeny. Longer half-lives are indicated as shades of green, medium as yellow, and shorter half-lives as shades of orange.

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