Overexpression of miR-509-5p and miR-1243 enhances the effect of gemcitabine. (a,b) Effect of a combination of miRNA and gemcitabine on cell index curves (RTCA) in Panc1 cells. A total of 5 nmo/L of miR-NC, miR-509-5p or miR-1243 was transfected, and cells were treated with gemcitabine (0.1, 1, 10, 50, 100 and 200 nmol/L) after 6 hours. Figure 5a shows 0.1 nmol/L gemcitabine-treated cells, and Fig. 5b shows 100 nmol/L gemcitabine-treated cells. Cell indexes were normalized with the last time point before treatment with gemcitabine. (c) Representative curves of the growth-suppressive effects at 120 hours following treatment with gemcitabine in cells transfected with miR-NC, miR-509-5p and miR-1243, and the IC50 in each miRNA transfectant.