miR-509-5p and miR-1243 induce an MET phenotype in several cancer cell lines. (a) The expression of miR-509-5p, miR-1243 and EMT marker genes was confirmed by qRT-PCR in Panc1 cells 72 hours after transfection of miR-509-5p and miR-1243; the data are the mean ± SD (bars) for duplicate experiments. (b) Immunofluorescent staining of E-cadherin (green) and DAPI (blue) in Panc1 cells after transfection of 10 nmol/L of miR-NC, miR-509-5p or miR-1243. (c) Western blot analysis of E-cadherin and Vimentin protein levels in Panc1, KP4-4 and MDA-MB-231 cells 72 hours after transfection of 10 nmol/L of miR-NC, miR-509-5p or miR-1243. (d) The expression of E-cadherin in Panc1 cells 72 hours after treatment with or without TGF-β (5 ng/ml) and simultaneous transfection with miR-NC, miR-509-5p and miR-1243. The number under the figure indicates the relative ratio based on the miR-NC expression level. (e) Western blot analysis of the EMT-related proteins ZO-1, ZEB1 and Snail in Panc1 cells 72 hours after transient transfection with 10 nmol/L of miR-NC, miR-509-5p and miR-1243. Student’s t-test was used for statistical analysis, and asterisks represent P < 0.05 versus miR-NC transfectants.