EMT-suppressive miRNAs are extracted using the function-based screening by combining the cell-based reporter system and an miRNA library in Panc1 clones. (a) Flow chart of the study. (b) Result of the function-based screening using Pre-miRTM miRNA Precursor Library-Human V15, which contains 1,090 miRNAs mimicking human mature miRNAs. The fluorescence intensity of ZsGreen1 was measured in duplicate with a fluorescence microplate reader. The relative fluorescence intensity in each transfectant was calculated using the fluorescence intensity in negative control cells and was normalized based on the cell survival rate, as measured by the crystal violet stain (Supplementary Table S1). The lower closed arrow indicates the 1090 miRNAs examined. (c) Venn diagram showing the overlap of six miRNAs between PEcadZsG-Panc1 #1 and #2. (d) The expression of E-cadherin in PEcadZsG-Panc1 #1 and parental Panc1 cells transfected with each candidate miRNA that was selected in the screening.