Tandem malonate-based glucosides (TMGs) for membrane protein structural studies

High-resolution membrane protein structures are essential for understanding the molecular basis of diverse biological events and important in drug development. Detergents are usually used to extract these bio-macromolecules from the membranes and maintain them in a soluble and stable state in aqueous solutions for downstream characterization. However, many eukaryotic membrane proteins solubilized in conventional detergents tend to undergo structural degradation, necessitating the development of new amphiphilic agents with enhanced properties. In this study, we designed and synthesized a novel class of glucoside amphiphiles, designated tandem malonate-based glucosides (TMGs). A few TMG agents proved effective at both stabilizing a range of membrane proteins and extracting proteins from the membrane environment. These favourable characteristics, along with synthetic convenience, indicate that these agents have potential in membrane protein research.

(LPDs) 26 , and β-peptide (BPs) 27 were developed as alternatives to small amphiphilic molecules. Some of these membrane-mimetic systems contain a patch of lipid bilayer stabilized by surrounding amphipathic agents, as exemplified by bicelles and nanodiscs (NDs) 28,29 . Despite their excellent efficacy toward protein stabilization, most of these large membrane-mimetic systems (e.g., amphipols and NDs) are not efficient at extracting proteins from the membranes, or have yet to produce high quality protein crystals. Small amphiphilic molecules tend to be more effective at extracting proteins from the membranes, but they are not usually as effective as the large membrane-mimetic systems at stabilizing membrane protein structures 29 . In addition, small glucoside detergents have been demonstrated to be inferior to their maltoside counterparts with respect to protein stabilization (e.g., OG vs DDM), but may be more suitable for crystallisation presumably due to the small size of the micelle 11,20 . Thus, it is particularly challenging to develop small glucoside detergents with enhanced protein-stabilizing efficacy relative to DDM, the gold standard conventional detergent. In the present study, we designed and synthesized novel glucosides by connecting two malonate-based core units via an alkyl or thioether linkage, designated alkyl chain-or thioether-linked tandem malonate-based glucosides (TMG-As/Ts) (Fig. 1). When these agents were evaluated for multiple membrane protein systems, TMG representatives conferred enhanced stability to most of the tested proteins compared to DDM, with the best detergent variable depending on the target protein.

Results
Design, synthesis and physical properties of TMG amphiphiles. The newly designed amphiphiles feature two alkyl chains and two branched diglucosides as tail and head groups, respectively (Fig. 1). These agents are structurally distinct from GNGs that we developed previously 21 . Both TMGs and GNGs share a central malonate-based unit, but the GNGs contain a single malonate-derived unit while the TMGs have two of these units linked by a short alkyl chain. [11] This difference results in variation in detergent inter-alkyl chain distance, the number of glucoside units, detergent geometry and detergent flexibility. The TMGs were divided into two groups according to the linker structure: TMG-As and TMG-Ts (Fig. 1). The TMG-As contain two malonate-derived units connected to each other via a propylene linker, different from the TMG-Ts with a thioether-functionalized linker (dimethyl sulfide linker). In addition, the two alkyl chains were introduced into the tandem malonate-based core via ether linkages (TMG-Ts) or directly (TMG-As). Since the optimal balance between hydrophilicity and hydrophobicity is known to be essential for effective stabilization of membrane proteins 30 , detergent alkyl chain length was also varied from C11 to C14. Both sets of the novel agents (TMG-As/Ts) were prepared using a straightforward synthetic protocol. The TMG-As were synthesized in five steps: alkyl connection of two malonate units, dialkylation and reduction of tetra-ester derivatives, glycosylation and global deprotection (see Supplementary scheme 1). The same number of synthetic steps was necessary for the preparation of the TMG-Ts (see Supplementary scheme 2). In this case, however, the initial three synthetic steps could be combined together to obtain the dialkylated tetra-ol derivatives in a single chromatographic separation, thus increasing synthetic accessibility of the TMG-Ts. The final agents could be prepared at an overall yield of more than 30% for both TMG sets without an optimization process (see Supplementary synthetic protocol). The convenient synthesis and high synthetic yield make preparation of these agents feasible in multi-gram quantity.
All the TMGs were water-soluble up to 10% and micelles formed by these agents were stable enough to give clear solutions for several months. These novel agents were physically characterized in terms of critical micelle concentrations (CMC) and the hydrodynamic radii (R h ) of their micelles. The CMCs were estimated using a hydrophobic fluorophore 31 while the hydrodynamic radii (R h ) were measured using dynamic light scattering . Two malonate units were connected via a propylene linker in the case of the TMG-As or via a dimethyl sulfide linker for the TMG-Ts. The alkyl chain length varied from C11 to C14 for both sets of TMGs, and this was incorporated into the detergent designation.
(DLS). The summarized results for both sets of the TMGs along with a conventional detergent (DDM) are presented in Table 1. The CMC values of the novel agents varied from 4 µM to 20 µM, all substantially smaller than DDM (170 µM), indicating that the TMGs have a 10-40 times higher tendency to self-aggregate than DDM. The CMC values of the TMGs decreased with increasing alkyl chain length, consistent with the general idea that hydrophobicity of the alkyl chain is the main factor in determining detergent CMC. For instance, TMG-A14, with the longest alkyl chain, gave a CMC value four times smaller than TMG-A11, with the shortest alkyl chain. No major difference was observed in the CMC values between the TMG-As and the equivalent TMG-Ts although the CMCs for the TMG-Ts were slightly higher than the TMG-As in this respect. The sizes of micelles formed by the TMGs increased from 3.1/3.0 to 3.8 nm with increasing alkyl chain length from C11 to C14, more or less comparable to that formed by DDM (3.4 nm; C12). The TMG-Ts tend to form smaller micelles than the TMG-As (TMG-T12 (3.1 nm) vs TMG-A12 (3.3 nm)). When DLS data were further analysed with regard to number-averaged size distributions, all of these detergents (TMG-As and TMG-Ts) produced a single set of micelle populations, indicative of high micellar homogeneity (see Supplementary Fig. 1).
TMG evaluation with a diverse set of membrane proteins. The newly prepared TMGs were first evaluated using the Rhodobacter (R.) capsulatus super-assembly, comprising light harvesting complex I and the reaction centre complex (LHI-RC) 32 . This complex is known to be particularly sensitive to protein denaturation  and this denaturation can be readily assessed by measuring the absorbance at 875 nm which detects the presence of cofactors (e.g., chlorophylls and carotenoids) embedded in the folded state 33 . For detergent evaluation, DDM-purified LHI-RC complex (80 μL) was diluted into buffer solutions (920 μL) containing individual detergents (TMG-As, TMG-Ts or DDM) to give final protein and detergent concentrations of 0.2 μM and CMC + 0.2 wt%, respectively. The residual amount of DDM is estimated to be ~0.005 wt%, assuming that a single membrane protein is surrounded by 400 DDM molecules. It was reported that the aggregation number of DDM in association with membrane proteins vary in a typical range of 100 to 400, depending on target proteins 34 . Thus, the estimated residual amount of DDM was considered negligible compared to that of the novel agents (0.005 vs 0.2 wt%). Protein stability was assessed by measuring protein absorbance at 875 nm (A 875 ) at regular intervals over the course of a 20-day incubation at room temperature. As can be seen in Fig. 2, all of the TMGs were clearly superior to DDM at preserving complex integrity. When the two groups of TMGs were compared, the TMG-Ts were a little better than the TMG-As. TMG-A14 was the worst novel agent, but still significantly better than DDM in this regard. When detergent concentration was decreased to CMC + 0.04 wt%, similar trends were observed although the efficacy difference between the TMGs and DDM was reduced (see Supplementary Fig. 2). It is notable that all the TMGs were effective at stabilizing the complexes at both tested detergent concentrations whereas DDM efficacy was significantly decreased at the higher concentration of CMC + 0.2 wt%. The novel agents were further evaluated with the uric acid-xanthine/H + symporter (UapA) from Aspergillus nidulans 35 . UapA has been structurally characterised and shown to exist as a closely associated dimer in DDM 36 . In order to assess protein stability, a thermal denaturation assay was carried out using a sulfhydryl-specific fluorophore, N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM) 37 . As fluorescence emission intensity increases with the amounts of unfolded proteins in samples, CPM serves as a protein unfolding sensor. For this assay, DDM-purified transporter was diluted 1:150 into buffer solutions containing individual amphiphiles (TMG-As, TMG-Ts, or DDM) to give final protein and detergent concentrations of 0.2 μM and CMC + 0.04 wt%, respectively. Assuming that 400 DDM molecules aggregated around a single UapA dimer, DDM concentration is estimated to become ~0.011 wt% after sample dilution, lower than that of the novel agents (CMC + 0.04 wt%). The changes in fluorescence intensity of the samples were monitored regularly during a 125-min incubation at 40 °C. All of the novel agents (TMGs) were significantly better than DDM at preserving the transporter in the folded state (Fig. 3). Again, the TMG-Ts appeared to behave slightly better than the TMG-As. Of all tested TMGs, the shortest alkyl chain TMGs (TMG-A11/T11) were the least effective. The suboptimal property of these C11 alkyl chain agents was further demonstrated when the detergents were used at CMC + 0.2 wt%. At this concentration, TMG-A11 and TMG-T11 were worse than and just comparable to DDM, respectively. The TMG-Ts are generally better than the TMG-As at maintaining the folded state of the transporter, with TMG-A14 and TMG-T13 being the best performing agents of the TMG-As and TMG-Ts, respectively (see Supplementary Fig. 3). This result suggests that the long alkyl chain TMGs (e.g., TMG-T13/A14) are more favourable than the short alkyl chain counterparts (e.g., TMG-T11/A11) at stabilizing the transporter. These long alkyl chain TMGs were better than MNG-3 (commercial name: LMNG), a widely used novel agent, at stabilizing the transporter. MNG-3 was only marginally better than DDM for this protein under the conditions tested ( Fig. 3 and Supplementary Fig. 3).
The new detergents were further tested with the bacterial leucine transporter (LeuT) from Aquifex aeolicus 38 . To start with, DDM-purified transporter (100 μL) was mixed with individual detergent-containing solutions (900 μL) to give final protein and detergent concentration of 0.2 μM and CMC + 0.04 wt%, respectively. Following the sample dilution, the residual amount of DDM is calculated to be ~0.030 wt% using the aggregation number of DDM (i.e., 226) specifically reported for LeuT 39 , lower than the concentration of the novel agents (CMC + 0.04 wt%). Protein stability was assessed by measuring the ability of the transporter to bind a radiolabeled substrate ([ 3 H]-leucine) using scintillation proximity assay (SPA) 40 . The substrate binding activity of the transporter was monitored at regular intervals during an incubation period of 10 days at room temperature ( Fig. 4a). At this low detergent concentration, the stability of the protein in the TMG-As varied substantially depending on the alkyl chain length; the TMG-As with a shorter chain (e.g., TMG-A11/C12) were comparable to DDM while TMG-A14 with the longest alkyl chain was the least stabilizing. TMG-A13 with one carbon unit shorter than TMG-A14 was a little worse than DDM. A similar result was obtained when detergent concentration was increased to CMC + 0.2 wt% (see Supplementary Fig. S4a). Again, TMG-A12 was the most stabilizing detergent of the TMG-As, followed by TMG-A11 and TMG-A13. The longest alkyl chain TMG (TMG-A14) was again the least stabilizing. At CMC + 0.04 wt%, all TMG-Ts were markedly better at retaining the activity of the transporter than both DDM and the TMG-As. The best performing agent was TMG-T12 (Fig. 4b). When detergent concentration was increased to CMC + 0.2 wt%, all TMG-Ts except TMG-T14 were better than DDM at retaining activity of the transporter (see Supplementary Fig. S4b). Based on these results, the C12 alkyl chain in the TMG architecture appeared to be optimal for transporter stability. Finally, in the absence of the TMGs (i.e., detergent-free condition), the ability of LeuT to bind the radiolabeled substrate was reduced to 25% of that of DDM by a 30-min incubation (see Supplementary Fig. S5). A further decrease in transporter activity was observed in the course of a 20-hour incubation. This result indicates that the estimated residual DDM (~0.030 wt%), although present at a higher concentration than the CMC (~0.0087 wt%), is not enough to preserve stability of the transporter. Thus, the presence of the individual TMGs appears to be essential for transporter stability.
The intriguing results of the TMGs encouraged us to test these agents with the human β 2 adrenergic receptor (β 2 AR), a G-protein coupled receptor (GPCR) 41 . The receptor stability was assessed via a ligand binding assay using the antagonist, [ 3 H]-dihydroalprenolol ([ 3 H]-DHA) 42 . The assay started with the 150-fold dilution of DDM-purified receptor into detergent solutions containing either DDM or individual TMGs (TMG-As and TMG-Ts) to reach final protein and detergent concentrations of 0.2 μM and CMC + 0.2 wt%, respectively. The residual DDM concentration, which is estimated to be ~0.0007 wt% by assuming 400 DDM molecules/receptor, was negligible compared to the final concentration of a novel agent (~0.2 wt%). Following a 30-min incubation to allow for complete detergent exchange, the ligand binding activity of the receptor was monitored. Some TMGs such as TMG-A13/A14 and TMG-T13/T14 were as effective as DDM at retaining receptor activity (Fig. 5a). Thus, these agents were selected for further analysis where receptor activity was monitored regularly over the course of 7-day incubation at room temperature. In this experiment, TMG-A13 and TMG-T13 were superior to DDM at maintaining receptor activity long-term, with TMG-A13 outperforming TMG-T13 (Fig. 5b). Similarly, TMG-A14 and TMG-T14 were superior to DDM although these agents were a little worse than DDM in terms of initial receptor activity (t = 0). Of the TMGs tested here, TMG-T14 was best at preserving receptor activity, followed by TMG-A13 and TMG-A14. When the DDM-solubilized receptor was diluted into TMG-free buffer solution (i.e., detergent-free condition), the receptor rapidly degraded over time, giving only ~10% residual protein activity after a 3-hour incubation (see Supplementary Fig. S6). This result indicates that the receptor cannot maintain structural and functional integrity in the absence of the individual TMGs. We selected two TMGs (TMG-A13 and TMG-T14) to further investigate these agents in terms of homogeneity of receptor-detergent complexes. A SEC result showed that each TMG produced monodisperse complexes with β 2 AR, similar to that formed by DDM (see Supplementary Fig. S7). This result implies that TMG-A13 and TMG-T14 may hold significant potential for GPCR study.
For each of the membrane proteins tested above, a conventional detergent (DDM) was first used to solubilize and purify the target protein and this DDM-purified protein was then diluted into individual detergent-containing solutions. Thus, mixed detergent systems containing small amounts of residual DDM were used for detergent efficacy comparison. As for LHI-RC and β 2 AR, the residual amounts of DDM (<0.005 wt%) were much smaller than those of the individual novel detergents (i.e., CMC + 0.04/0.2 wt%) and even smaller than CMC value of DDM (~0.0087 wt%). As for two of the transporters (UapA and LeuT), the residual DDM amounts are estimated to be 0.011 and 0.030 wt%, respectively. These concentrations are comparable to the low concentrations of the TMGs (CMC + 0.04 wt%), but are substantially smaller than the high TMG concentrations (CMC + 0.2 wt%). As observed in the detergent-free condition, the residual amount of DDM (~0.030 wt%) was too small to maintain LeuT activity. In the case of UapA, as we have used the highest predicted aggregation number for DDM associated with the protein (400 molecules of DDM/UapA dimer), it is possible that in reality this is lower. Furthermore, the effect of residual DDM on protein stability should be similar from one sample to another as detergent evaluation was carried out in each case using a side-by-side comparison. Thus, the residual amount of DDM is unlikely to interfere with evaluation of the novel agents for the membrane proteins studied here. However, we can't completely exclude that residual DDM is having an effect on our analysis of these proteins.
In order to assess this further, all the TMG agents were used to extract the Salmonella typhimurium melibiose permease (MelB St ) directly from E. coli membranes 43,44 . The membrane fractions were mixed with 1.5% DDM or individual TMG detergents (TMG-As or TMG-Ts) on ice and the resulting solutions were then incubated for 90 min at four different temperatures (0, 45, 55, and 65 °C). The amount of MelB St extracted and stabilised by each detergent was analyzed through SDS-PAGE and Western blotting after separation by ultracentrifugation (Fig. 6a), and expressed as a percentage of the total amount of MelB St initially present in the untreated membrane (Fig. 6b)  evaluated in terms of MelB function monitored by measuring FRET from tryptophan residues to 2′-(N-dansyl) aminoalkyl-1-thio-β-d-galactopyranoside (D 2 G) bound to the protein (i.e., Trp→ D 2 G FRET) 45 . Upon addition of D 2 G, a functional MelB St gives an increase in fluorescence intensity induced by Trp→ D 2 G FRET that can be reversed by adding a non-fluorescent sugar substrate (i.e., melibiose). Upon addition of melibiose, the DDM-solubilized MelB St gave a large reversal in the FRET signal while the TMG-A12 or TMG-A13-solubilized MelB St appeared to be less responsive in this regard ( Fig. 6c and d). A similar trend was observed for MNG-3-solubilized MelB St in a previous study 46 . When we used MelB from Escherichia coli (MelB Ec ), known to be less stable than MelB St 46 , DDM failed to give functional protein. In contrast, TMG-A12 or TMG-A13 resulted in a functional MelB Ec as demonstrated by large changes in FRET signal. These results indicate that these novel agents, particularly TMG-A12, are effective at maintaining MelB functionality as well as solubility.

Discussion
Detergent efficacy can be considerably affected by a minor change in detergent structure. Despite the small variations in the chemical structures, the different TMGs showed marked variations in membrane protein stabilization. The TMG-Ts were overall better than the TMG-As at stabilizing all the tested membrane proteins except MelB St . In addition, the best detergents varied depending on the individual target proteins. TMG-T12 and TMG-T13 were best for LeuT and UapA stability, respectively, while TMG-A13/TMG-T14 and TMG-A12 were best for β 2 AR and MelB St , respectively. It is notable that short alkyl chain TMGs (e.g., TMG-A11/A12 and TMG-T11/T12) tended to be favorable for LeuT stability while long alkyl chain TMGs (e.g., TMG-A13/A14 and TMG-T13/T14) were generally advantageous for β 2 AR and UapA stability. Of the TMG-As and TMG-Ts, TMG-A12/TMG-A13 and TMG-T13/TMG-T14 were the best overall at maintaining protein stability; these agents were superior or at least comparable to DDM in maintaining the stability of all the tested proteins. The current paradigm is that the most suitable detergent tends to be protein-specific. Because there is a large diversity in the tendency of membrane proteins to aggregate/denature and a substantial variation in protein dimensions, it is unlikely that a single or even a small number of detergents can act as a magic bullet for all or most membrane proteins. However, our result indicates that it may be possible to develop a detergent suitable for a large number of membrane proteins. Detergent efficacy also tended to depend on detergent concentration. In the current study, detergent concentration of CMC + 0.04 or 0.2 wt% was used for detergent evaluation with LHI-RC, UapA and LeuT. As for LHI-RC and LeuT, the novel agents showed reduced protein stability at a high concentration (CMC + 0.2 wt%). A similar trend was observed for UapA, although it is less clear. This result is consistent with the notion that excess detergent micelles produced by increased detergent concentration is detrimental for protein stability 47 . Note that an optimal detergent concentration could significantly vary depending on the property and concentration of a target membrane protein.
Currently, there are a very limited number of glucoside detergents that confer consistently greater stability to a range of membrane proteins than DDM; OG is known to be significantly less effective at stabilizing membrane proteins than DDM 11 and the most widely used novel glucoside agent, GNG-3 (commercial name: OGNG), was shown to be worse than DDM in terms of stabilizing a range of membrane proteins 21 . However, due in part to the presence of the comparatively small hydrophilic group, OG is widely used for membrane protein crystallization. In addition, GNG-3 has contributed to the crystal structure determination of several membrane proteins in the last four years [48][49][50][51] . Thus, it is of great importance to develop additional glucoside detergents with enhanced protein stability compared to DDM or GNG-3. Achieving enhanced stability for multiple membrane proteins is challenging as detergent efficacy tends to be protein-specific as described above. However, on the basis of our previous and current studies 21 , we seem to have achieved this with at least some of the TMGs (e.g., TMG-A12/A13 and TMG-T13/T14) as these agents were shown to be more effective than both DDM and GNG-3 at stabilizing a range of membrane proteins.
In summary, two groups of novel glucoside agents were prepared by connecting two malonate-based units with a propylene or dimethyl sulfide spacer. When these TMG agents were evaluated with five membrane proteins in terms of protein stabilization, the TMG-Ts were generally superior to the TMG-As for three membrane protein systems (LHI-RC complex, UapA and LeuT). An opposite trend was observed for MelB St stability while the comparable efficacy of the TMGs was found for β 2 AR. More importantly, some of the TMGs (TMG-A12/TMG-A13 and TMG-T13/TMG-T14) were more effective than or at least comparable to DDM at maintaining the stability of the tested membrane proteins here, suggesting wide applicability of these agents. As glucoside detergents are advantageous for membrane protein structural study, these synthetically accessible TMG agents have potential for membrane protein research.

Methods
R. capsulatus superassembly stability assay. The R. capsulatus superassembly expressed in the engineered strain of Rhodobacter capsulatus was solubilized and purified according to the reported protocol 33 . A 10 mL aliquot of the frozen membranes was thawed and homogenized using a glass tissue homogenizer at room temperature. The homogenate was incubated with mild agitation at 32 °C for 30 min. After the addition of 1.0 wt% DDM, the homogenate was incubated for an additional 30 min at 32 °C. Following ultracentrifugation, the supernatant containing the solubilized LHI-RC complexes was collected and incubated with Ni 2+ -NTA resin at 4 °C for one hour. The resin was loaded into 10 His-SpinTrap columns separately and washed twice with 500 μL binding buffer (10 mM Tris (pH 7.8), 100 mL NaCl, 1 × CMC DDM). A binding buffer containing 1 M imidazole (2 × 300 μl) was used to elute DDM-purified LHI-RC complex. 80 μL of the DDM-purified LHI-RC complex was diluted into 920 μL of individual detergent solutions; TMG-As (TMG-A11, TMG-A12, TMG-A13 and TMG-A14), TMG-Ts (TMG-T11, TMG-T12, TMG-T13 and TMG-T14) or DDM to reach a final detergent concentration at CMC + 0.04 wt% or CMC + 0.2 wt%. Sample dilution was carried out for one hour and the complex was incubated at room temperature for 20 days. Protein stability was measured at regular intervals during the incubation by measuring UV-Visible spectra of the samples in the range of 650 to 950 nm.
UapA thermal denaturation assay. UapAG411V Δ1-11 from Aspergillus nidulans was expressed as a C-terminal GFP fusion protein in the FGY217 strain of Saccharomyces cerevisiae. The UapA was isolated and purified in sample buffer (20 mM Tris (pH 7.5), 150 mM NaCl, 0.03% DDM, 1 mM xanthine) according to the reported protocol 52 . The protein was concentrated to approximately 10 mg/mL using a 100 kDa molecular weight cut off filter (Millipore). The protein was diluted 1:150 into buffer containing either TMG-As (TMG-A11, TMG-A12, TMG-A13 and TMG-A14), TMG-Ts (TMG-T11, TMG-T12, TMG-T13 and TMG-T14) or DDM to give final detergent concentrations of CMC + 0.04 wt% or CMC + 0.2 wt% in Greiner 96-well plates. The CPM dye (Invitrogen) stored in DMSO (Sigma) was diluted in dye buffer (20 mM Tris (pH 7.5), 150 mM NaCl, 0.03% DDM, 5 mM EDTA) and 3 μL of the dye buffer was added to each sample. Protein stability was measured by incubating the reaction mixture for 125 min at 40 °C, starting from 30 min after sample dilution. The fluorescence emission was recorded using a microplate spectrofluorometer set at excitation and emission wavelengths of 387 nm and 463 nm, respectively. The relative amounts of folded proteins were plotted against time using GraphPad Prism.
Scientific RepoRts | 7: 3963 | DOI:10.1038/s41598-017-03809-3 Finally, 1.5 mg/mL protein stock was diluted in identical buffer without DDM and imidazole, but supplemented with individual TMG-As (TMG-A11, TMG-A12, TMG-A13 and TMG-A14), TMG-Ts (TMG-T11, TMG-T12,  TMG-T13 and TMG-T14) or DDM (a positive control) to reach a final concentration of CMC + 0.04 wt% or CMC + 0.2 wt%. As a negative control, the protein stock was diluted into a detergent-free buffer solution. The samples stood for one hour to allow detergent exchange and were then stored for 10 days at room temperature, centrifuged at the indicated time points and the ligand binding activity was measured using [ 3 H]-Leu via scintillation proximity assay (SPA) 40 . SPA was performed at the above-mentioned detergent concentrations with 5 μL of the respective protein samples, 20 nM [ 3 H]-Leu and 1.25 mg/mL copper chelate (His-Tag) YSi beads (both from Perkin Elmer, Denmark) in buffer containing 450 mM NaCl. [ 3 H]-Leu binding was determined via MicroBeta liquid scintillation counter (Perkin Elmer). β 2 AR long-term stability assay. β 2 AR was isolated and purified in 0.1% DDM according to the reported protocol 42 . Briefly, β 2 AR was expressed in Sf9 insect cells infected with baculovirus and solubilized in 1% DDM. The DDM-purified β 2 AR was added to individual TMG-containing buffers (TMG-As (TMG-A11, TMG-A12,  TMG-A13 and TMG-A14), TMG-Ts (TMG-T11, TMG-T12, TMG-T13 and TMG-T14), GNGs (GNG-2 and GNG-3), or DDM to make a final concentration at CMC + 0.2 wt%. As a control, the DDM-purified β 2 AR was diluted into a detergent-free buffer. After allowing 30-min sample dilution, β 2 AR solubilized in individual detergents was stored for 6 or 7 days at room temperature and ligand binding ability was assessed at regular intervals over this period by incubating the samples with 10 nM [ 3 H]-dihydroalprenolol (DHA) supplemented with 0.5 mg/ml BSA for 30 min at room temperature. The combined mixture was loaded onto a G-50 column and the flowthrough was collected in 1 ml binding buffer (20 mM HEPES pH 7.5, 100 mM NaCl, containing 0.5 mg/mL BSA and 20 × CMC individual detergents). A further 15 ml scintillation fluid was added and receptor-bound Determination of MelB stability and functionality. The E. coli DW2 strain (ΔmelB and ΔlacZY) harboring pK95ΔAHB/WT MelB St /CH 10 , encoding the wild-type melibiose permease of Salmonella typhimurium (MelB St ) carrying a C-terminal 10-His tag was used for this study 43,53 . Membranes containing MelB St (~10 mg/ mL) in a buffer (20 mM sodium phosphate, pH 7.5, 200 mM NaCl, 10% glycerol and 20 mM melibiose) were treated with individual detergents [DDM, TMG-As (TMG-A11, TMG-A12, TMG-A13 and TMG-A14), or TMG-Ts (TMG-T11, TMG-T12, TMG-T13 and TMG-T14)] at 1.5% (w/v). The samples were then incubated at four different temperatures (0, 45, 55, and 65 °C) for 90 min, followed by ultracentrifugation at 355,590 g in a Beckman OptimaTM MAX ultracentrifuge using a TLA-100 rotor for 45 min at 4 °C. An equal amount of total membrane proteins (20 μg) was analysed on an SDS-15% PAGE gel. MelB St was detected by immunoblotting with a Penta-His-HRP antibody (Qiagen, Germantown, MD). For the Trp → D 2 G FRET assay, the right-side-out (RSO) membrane vesicles were prepared from E. coli DW2 cells containing MelB St or MelB Ec by osmotic lysis 43,54 . D 2 G (2′-(N-dansyl)aminoalkyl-1-thio-β-d-galactopyranoside) was provided by Drs. Gerard Leblanc and H. Ronald Kaback. RSO membrane vesicles in buffer (pH 7.5) containing 100 mM KP i and 100 mM NaCl at a protein concentration of 1 mg/ml were treated with 1.0% DDM, TMG-A12, or TMG-A13 at 23 °C for 30 min and subjected to ultracentrifugation using a TLA 120.2 rotor at >300,000 g for 45 min at 4 °C. The supernatants were directly used for the Trp → D 2 G FRET experiments using an Amico-Bowman Series 2 (AB2) Spectrofluorometer. Trp residues were excited at 290 nm and emission was recorded at 465 nm and 490 nm for MelB Ec and MelB St , respectively. On a time trace, 10 μM D 2 G was added at 1-min time point, and excess amount of melibiose or equal volume of water were added at 2-min time point.