Capacity of human neutrophils for PGE2 production is sex-dependent. PGE2 production in human neutrophils from male and female donors after LPS or A23187 stimulation. Neutrophils from male and female donors were incubated with (A) LPS (1 µg/ml) for 0, 0.5, 3 and 20 hrs, or (B) with 0.5 µM A23187 for 0, 5, 15, 30, 60, 120, and 240 min. The reactions were stopped on ice, and PGE2 levels were measured by ELISA. (C) After selected time points (0, 5, 15, 30, 60, 120 min) upon stimulation with A23187 (0.5 µM), free 3H-AA content in the supernatant of 3H-AA-pre-labelled neutrophils was evaluated by scintillation counting. Values represents means ± S.E.M of n = 3–6 experiments each in duplicate. #p < 0.05, ##p < 0.01, male vs female cells; two-way ANOVA plus Bonferroni.