Sex differences in eicosanoid biosynthesis in carrageenan-induced pleurisy in rats. Rats were treated with carrageenan (intrathoracic injection), sacrificed at the given time points, and (A) LTB4 and (B) PGE2 in the thoracic exudates were measured by EIA and RIA, respectively. (C) 6-keto-PGF1α levels in thoracic exudates were measured 4 hrs after carrageenan injection by EIA. Time zero (T0) corresponds to untreated rats. Values represent means ± S.E.M; n = 6 rats/T0; n = 13 rats/2 hrs; n = 6 rats/4 hrs; n = 6 rats/8 hrs; #p < 0.05; ###p < 0.001, male vs female; two-way ANOVA plus Bonferroni (A and B) and two-tailed Student’s t test (C).