Introduction

Tuberculosis, caused by Mycobacterium tuberculosis (M. tb), continues to be a major global health problem, causing 1.5 million deaths and 9.6 million new infections in 2014. In the majority of individuals infected with M. tb, the bacteria establish a latent, asymptomatic infection that can persist for decades1, 2. About 5–10% of these individuals will develop active disease in their lifetime and the risk of reactivation is markedly increased by host immunosuppression3. About one-third of the world’s population is latently infected, representing a large reservoir for reactivation4. Understanding the genetic programs that facilitate the entry of M. tb into and emergence from latency will facilitate the development of novel therapeutic strategies.

During the course of infection, M. tb must encounter heterogeneous host environments, including the high oxygen tension in the lung alveolus5, the reactive oxygen and nitrogen species generated in activated macrophages6, as well as the nutrient depleted and hypoxic center of tuberculous granulomas7, 8. How M. tb survives these diverse environments is not fully understood. Of particular interest is the response of M. tb to changes of oxygen tension. Studies from human and animals suggest an intimate link between oxygen tension and the outcome of M. tb infection9,10,11,12,13,14. As such, hypoxia is considered a major stimulus that triggers M. tb latency9, 15 and is one of the most frequently used in vitro conditions to mimic the environment of human granulomas15,16,17. Accordingly, sets of M. tb genes that respond to hypoxia have been identified, such as the extensively studied heme-based DosR/S/T regulon18,19,20,21.

Little is known about the reactivation of M. tb from the persistent state, and presumably access to oxygen is an important requirement. Consistent with this notion, reactivation of latent TB in humans occurs most frequently in the upper lobes of the lung, the most oxygenated region of the body9. In the later stage of active pulmonary TB, lung cavities that connect to airways provide oxygen-rich environments, allowing M. tb to reach high density and subsequent spread22. Therefore, knowledge on the response of M. tb to oxygen exposure is critically important for understanding the pathogenesis of M. tb.

Several recent studies demonstrate that mycobacterial WhiB4 is an oxygen-sensitive transcription factor. WhiB4 is a member of the WhiB superfamily transcription factors that are conserved in actinomycetes, including many mycobacterial species such as M. tb and M. marinum 23,24,25,26,27,28. WhiB4 contains the highly conserved Cys-X14-22-Cys-X2-Cys-X5-Cys motif, which forms a [4Fe-4S] cluster in the holoenzyme29, 30. Upon exposure to O2, WhiB4 rapidly (within minutes) loses the [4Fe-4S] cluster and leads to an oxidized apo-WhiB4 containing disulfide bonds. Importantly, the oxidized form of WhiB4 has the strongest DNA binding activity30. The response of WhiB4 to oxygen exposure occurred much more rapidly than other WhiB proteins (e.g., WhiB1 and WhiB3), making it an oxygen sensor30,31,32. The ability to rapidly respond to oxygen exposure and activate DNA binding activity implies an active role of WhiB4 in mycobacterial virulence. However, deletion of whiB4 from M. tb resulted in hypervirulence in the lungs of guinea pigs, which was attributed to the increased expression of antioxidant enzymes including alkyl hydroperoxide reductases AhpC and AhpD in this strain30. Therefore, WhiB4 in M. tb is primarily involved in modulation of oxidative stress response and is apparently not required for virulence. In this study, we found that WhiB4 plays a different role in M. marinum. Unlike in M. tb, inactivation of whiB4 in M. marinum led to loss of virulence in zebrafish, a natural host of M. marinum, and impaired replication in macrophages. RNA-seq data revealed that WhiB4 positively regulates a large number of pe/ppe gene family in M. marinum, providing an explanation for the essential role of WhiB4 in M. marinum virulence. Our study provides new insight into the biological function of WhiB4 and also indicates a different role for WhiB4 in different pathogenic mycobacteria.

Results

M. marinum ΔwhiB4 is defective in cording formation

We have isolated a transposon inactivated whiB4 mutant strain of M. marinum, which was identified in a screen of mutation library for altered colony morphology. The transposon was inserted at a TA site in the promoter region of whiB4, 28 bp upstream of the start codon. Since the transposon inactivated transcription of whiB4, we referred this strain ΔwhiB4. The mutant displayed aberrant crustose colony morphology as compared to the smoother, glossier morphology of the WT strain (Fig. 1A). Previously, we showed that changes of colony morphology of M. marinum are often associated with defective biosynthesis of lipooligosaccharides (LOSs) in the cell wall33. Consistently, a recent study found that deletion of whiB4 from M. marinum E11 strain resulted in rough colony morphology and diminished levels of LOSs34.

Figure 1
figure 1

M. marinum ΔwhiB4 exhibited altered colony morphology and was defective in cording formation. (A) M. marinum ΔwhiB4 exhibited dry and rough colony morphology on 7H11 agar, as compared to as compared to the smoother, glossier, morphology of WT or the complemented strain. (B) Cells of WT or the complemented strain formed the serpentine cords, which were not observed for ΔwhiB4.

Importantly, we found that M. marinum ΔwhiB4 is defective in cording formation (Fig. 1B), a phenotype not previously described. When grown in liquid media, the WT strain formed serpentine cords that are characteristic of pathogenic mycobacteria, including M. marinum. This phenotype was abolished in ΔwhiB4. Complementation of ΔwhiB4 with a plasmid pNBV1 containing intact whiB4 gene restored both the colony morphology and cording formation, indicating that inactivation of whiB4 gene is responsible for these phenotypes.

M. marinum ΔwhiB4 is defective in intracellular replication

Cording morphology was first described by Robert Koch, and previous studies have revealed a correlation between cording and virulence35,36,37. The defective cording phenotype of the ΔwhiB4 strain prompted us to examine if inactivation of whiB4 affects virulence of M. marinum. We first performed macrophage infection experiments to determine the intracellular growth of ΔwhiB4. RAW macrophages were infected with WT, ΔwhiB4, or the complemented strain of M. marinum and the intracellular growth of bacteria were assayed by enumerating the bacterial number (colony forming unit, CFU) at different time points post-infection. Both WT and the complemented strain grew substantially inside macrophages. By contrast, the growth of ΔwhiB4 inside macrophages was significantly reduced (Fig. 2). All three strains exhibited equivalent growth in 7H9 media. Taken together, these data suggest that whiB4 is required for the intracellular replication of M. marinum.

Figure 2
figure 2

M. marinum ΔwhiB4 exhibited reduced intracellular survival. RAW macrophage cells were infected with M. marinum strains and the intracellular survival of each strain was determined by determining bacterial CFU at indicated time points post infection. Data are plotted as mean ± SEM (n = 3), and are representative of two independent experiments. The CFU of ΔwhiB4 at day 4 and 5 were significantly lower than that of WT (*p < 0.05; **p < 0.01), or the complemented strain (p < 0.001) as analyzed by two-way ANOVA.

M. marinum ΔwhiB4 is attenuated in zebrafish

Zebrafish are a natural host of M. marinum and have been widely used as a laboratory model for studying M. marinum infection, which manifests both acute disseminated disease and chronic persistent infection38. To assess the role of whiB4 in virulence, adult zebrafish (15 per group) were infected with WT, ΔwhiB4, or the complemented strain of M. marinum, and were monitored for survival. The median survival time of fish infected with WT and the complemented strain was 18- and 17- days, respectively (Fig. 3A). By contrast, all zebrafish infected with ΔwhiB4 survived during the 40-day course of experiments. Log-rank analysis indicated that the survival curve of the fish group infected with ΔwhiB4 was significantly different to the groups of fish infected with WT (p = 0.0005) or the complemented strain (p < 0.0001). There was no significant difference between the survival curves of fish infected with WT and the complemented strain (p = 0.08).

Figure 3
figure 3

M. marinum ΔwhiB4 was attenuated in zebrafish. (A) Zebrafish (15 per group) were infected with indicated strain at 104 CFU bacteria per fish, and were monitored for mortalities over a 40-day period. The survival curves were plotted using the Kaplan-Meier method and differences between curves were analyzed using the log-rank test. All fish infected with ΔwhiB4 or PBS survived. The survival curve of the ΔwhiB4 group was significantly different to those of WT (p = 0.0005) or the complemented strain (p < 0.0001). A similar result was found in another independent experiment (B).

Fish infected with ΔwhiB4 also exhibited significantly less severe pathology than those infected with WT or the complemented strain. At week 3 post infection, fish infected with WT or the complemented strain exhibited severe tissue damage in multiple organs, including livers, kidneys and intestines. Numerous bacteria were detected in these organs. As an example, fish liver infected with WT M. marinum is shown (Fig. 4A,B). The bacteria had spread extensively and there were no organized granulomas observed at this stage of infection. By contrast, there was no discernible pathology in livers or other organs of fish infected with ΔwhiB4 at the same period (week 3 post infection) (Fig. 4C). A few granulomas were detected in the livers of fish infected with ΔwhiB4 at week 4 post infection (Fig. 4D,E). The granulomas were well organized with bacteria contained inside (Fig. 4F), indicating a successful control of bacterial spread by the host. Taken together, these results demonstrated that inactivation of whiB4 in M. marinum greatly reduced its virulence in zebrafish.

Figure 4
figure 4

M. marinum ΔwhiB4 caused less severe pathology. Histological analysis of fish tissues from the experiment described in Fig. 3A. (A,B) Liver sections of fish infected with WT M. marinum at 3 weeks post-challenge. Samples were analyzed with hematoxylin and eosin (H&E) staining (A) and Ziehl-Neelsen method of acid fast staining (B). Both are 20× magnification. (C) H&E staining of liver sections of fish infected with ΔwhiB4 at 3 weeks post-infection (4× magnification). (D,E) Liver sections of fish infected with ΔwhiB4 at 3 weeks post-infection (10× magnification) and stained with H&E (D) or Ziehl-Neelsen (E). The well-organized granuloma observed in (E, white star) was zoomed in for better visualization (F), 40× magnification.

A similar result was found in another independent experiment (Fig. 3B). The median survival time of fish infected with WT or the complemented strains was both 28 days, whereas 13 out of 14 fish infected with ΔwhiB4 survived. Consistently, there was a significant difference between the survival curve of ΔwhiB4 infected group and groups infected with WT (p = 0.007, log-rank analysis) or the complemented strain (p = 0.003). There is no significant difference between the survival curves of fish infected with WT and the complemented strain (p = 0.98).

WhiB4 regulates the pe/ppe gene family in M. marinum

To understand the potential mechanisms of WhiB4-mediated virulence in M. marinum, we performed RNA-seq analysis and compared global transcripts between WT and ΔwhiB4 grown in 7H9 culture media. Two biologically independent experiments were performed and there was excellent agreement between the experiments. The correlation coefficient of total FPKM (Fragments Per Kilobase of exon model per Million mapped reads) counts between two biologically independent samples of WT was 0.99. Similarly, the correlation coefficient between two independent samples of ΔwhiB4 was 0.98. Averaged FPKM data of each strain from the two independent experiments were used for comparative gene expression analysis. RNA-seq revealed that the expression levels of 387 genes were altered by ≥2 fold in ΔwhiB4, and the differences were statistically significant (Supplementary Table S1). Of these, 257 genes were downregulated and 130 genes were upregulated in ΔwhiB4 compared to WT. The whiB4 transcript in ΔwhiB4 was 15-fold less than in WT, confirming the inactivation of whiB4 in the transposon insertion mutant. A number of genes (21 genes) in the biosynthetic locus of LOSs (MMAR_2307-MMAR_2354) were downregulated33, providing additional genetic evidence for the diminished LOSs in M. marinum ΔwhiB4 observed in a previous study34.

Remarkably, 98 genes that are differentially expressed in ΔwhiB4 encode the PE/PPE family proteins (Supplementary Table S2), suggesting that the pe/ppe gene family are significantly enriched in the WhiB4 regulon (the hypergeometric p = 1.8e−45). PE family proteins are characterized by the presence of a proline-glutamic acid (PE) motif at positions 8 and 9 within a highly conserved N-terminal domain consisting of around 110 amino acids. Similarly, PPE family proteins contain a proline-proline-glutamic acid (PPE) at positions 7–9 in a highly conserved N-terminal domain of approximately 180 amino acids39. The C-terminal domains of both PE and PPE family proteins are highly variable in both size and sequence, and often contain repetitive sequences. Based on the C-terminal sequence, PE/PPE family proteins can be divided into subfamilies39, 40. PE-PGRS is the largest PE subfamily and is characterized by a C-terminal domain that contains multiple tandem repeats of a glycine-glycine-alanine (GGA) or a glycine-glycine-asparagine (GGN) motif. Likewise, the PPE-MPTR subfamily contains major polymorphic tandem repeats at the C-terminal. The M. marinum genome contains 281 pe/ppe genes, which encodes 27 PE, 148 PE-PGRS, and 106 PPE proteins41. About 25.3% (98 out of 387) of genes whose expression changed in ΔwhiB4 are pe/ppe genes, which is ~5 fold of the percentage (5.2%, 281 out of 5424) of total pe/ppe genes present in the M. marinum genome, suggesting that WhiB4 specifically regulates the expression of these multigenic families of genes.

WhiB4 primarily acts as a positively regulator of the pe/ppe gene family. Of the 98 pe/ppe genes differentially expressed in ΔwhiB4, 79 were downregulated and 19 were upregulated. Moreover, WhiB4 predominantly regulates the pe-pgrs gene subfamily. Sixty-seven pe-pgrs genes were differentially expressed in ΔwhiB4, including 55 downregulated and 12 upregulated. Taken together, these results indicated that WhiB4 is a major regulator of the pe/ppe gene family, especially pe-pgrs genes.

Accumulated evidence suggests that the PE/PPE family proteins play a critical role in mycobacterial pathogenesis (reviewed in ref. 42). PE-PGRS30 and PE-PGRS62 were shown to inhibit macrophage phagosomal maturation43, 44, and PE-PGRS30 was required for the full virulence of M. tb in mice44. Interestingly, the expression levels of pe-pgrs62 and pe-pgrs30 were reduced 18.6- and 3.3-fold, respectively in ΔwhiB4 compared to that in WT, which provides a partial explanation for the reduced intracellular replication and diminished virulence of ΔwhiB4.

Many PE/PPE proteins have been shown to modulate host immune responses (reviewed in refs 45 and 46). Excessive proinflammatory responses can lead to deleterious effects for the host. In active TB, emerging evidence suggests that a high level of TNF could accelerate the disease progression47,48,49,50,51,52. Interestingly, we found that ΔwhiB4 induced lower levels of pro-inflammatory response, decreasing the production of TNF by macrophages at early time points (Fig. 5). The dampening immune response by ΔwhiB4 is consistent with the reduced histopathology in fish infected with this mutant (Fig. 4).

Figure 5
figure 5

M. marinum ΔwhiB4 was less potent inducing TNF. RAW 264.7 macrophage cells were infected with mycobacteria at MOI of 10. At 3, 12, and 24-hr post infection, cell-free supernatants were collected and the levels of TNF were determined by enzyme-linked immunosorbent assay (ELISA). Data are plotted as mean ± SEM (n = 3), and are representative of two independent experiments. The level of TNF secretion induced by ΔwhiB4 was significantly lower than that of WT or the complemented strain (***p < 0.001; two-way ANOVA).

WhiB4 differentially regulates antioxidant systems in M. marinum

The expression of several antioxidant enzymes were upregulated in ΔwhiB4 of M. marinum (Supplementary Table S1), including superoxide dismutase SodA, catalase/peroxidase KatE, and peroxidoxin BcpB. This is consistent with a previous finding that several genes associated with antioxidant systems were upregulated in M. tb ΔwhiB4, including ahpC and ahpD encoding alkyl hydroperoxide reductases30. The finding that WhiB4 negatively regulates the expression of antioxidant enzymes is somewhat difficult to reconcile with the positive requirement of WhiB4 in M. marinum virulence. Interestingly, a recently identified mycothiol-dependent oxidoreductase (mycoredoxin-1, Mrx1, MMAR_1363)53 was downregulated in ΔwhiB4 of M. marinum (Supplementary Table S1). Mycobacteria do not contain glutathione but produce functionally equivalent mycothiol that is present in millimolar concentrations. Mycothiol is therefore a major redox buffer against oxidative stress54. The ability of Mrx1 to couple the reducing power of mycothiol to reduce oxidized cellular proteins was recently demonstrated in AhpE of M. tb 55. It is conceivable that Mrx-1 may use mycothiol as a cofactor and reduce WhiB4. Based on these observations, we propose a model to explain the physiological function of WhiB4 in M. marinum (Fig. 6). In the presence of oxygen, the [4Fe-4S] cluster in WhiB4 is quickly destroyed and oxidized to disulfide bonds, which activates the DNA binding activity of WhiB430. Oxidized WhiB4 would activate the transcription of pe/ppe genes, which would facilitate bacterial intracellular replication and survival in the host. At the same time, oxidized WhiB4 would repress the expression of antioxidant enzymes and consequently maintain a relatively oxidizing environment. As a result, more oxidized WhiB4 would be generated. As these processes continue, the accumulation of Mrx1, which is dependent on oxidized WhiB4, would form a negative feedback regulation. Mrx1 would use mycothiol as a cofactor to reduce oxidized WhiB4 and eventually lead to the formation of [4Fe-4S]-containing WhiB4. The holo-WhiB4 would then lose its DNA binding activity and antioxidant enzymes (SodA, KatE) would be de-repressed, which would reduce the oxidative stress and return the system to its original state.

Figure 6
figure 6

A proposed model for the physiological role of WhiB4 in M. marinum. See text for explanations.

Discussion

In this study, we found that WhiB4 plays similar yet distinct roles in M. marinum and M. tb. WhiB4 modulates the oxidative stress response in M. marinum by repressing the expression of antioxidant enzymes including SodA and KatE, which is similar to a previous finding of WhiB4 in M. tb 30. However, WhiB4 is required for virulence in M. marinum. M. marinum ΔwhiB4 exhibited impaired replication in macrophages and diminished virulence in zebrafish. By contrast, M. tb ΔwhiB4 exhibited enhanced replication in macrophages and increased virulence in lungs of guinea pigs, although its dissemination to spleens was reduced30. Sequence analysis reveals that WhiB4 from M. tb and M. marinum shares an overall 83% sequence identity (amino acids), and 100% identity in the Cys-X14-22-Cys-X2-Cys-X5-Cys motif. Therefore, the small sequence variation between WhiB4 of these two organisms cannot account for the difference in physiological function. Our finding that WhiB4 plays a different role in virulence of different pathogenic mycobacteria is not without precedent; a previous study found that deletion of whiB3, another member of the whiB gene family, in two nearly identical organisms M. tb and M. bovis, resulted in different virulence phenotypes in guinea pigs56. Taken together, these studies suggest that although the WhiB family proteins are highly conserved among mycobacteria, they could play distinct physiological roles in specific mycobacterial species.

The different role of WhiB4 in virulence of M. marinum and M. tb likely arises from the difference of the WhiB4 regulon in these organisms. Strikingly, we found that a large number of pe/ppe genes (98) were regulated by WhiB4 in M. marinum. By contrast, only 3 pe/ppe genes (pe35, ppe19, ppe68) were regulated by WhiB4 in M. tb 30. The pe/ppe gene family represent one of the most intriguing aspects of mycobacterial genomes. The pe/ppe gene family was initially discovered in M. tb, comprising approximately 10% of M. tb genome39. Since then, pe/ppe genes have been identified in many pathogenic mycobacteria including the M. tb complex (MTBC, including M. tb, M. africanum, M. canettii, M. bovis and M. microti), M. leprae, M. avium, M. marinum and M. ulcerans. M. marinum has the most extensive repertoire of pe/ppe genes reported (281 in M. marinum compared with 169 in M. tb and 115 in M. ulcerans)41. Nonpathogenic mycobacteria have few pe/ppe genes (e.g., 10 in M. smegmatis). Although the origin of the pe/ppe gene family is unknown, the ancestral pe/ppe genes are associated with the type VII secretion system, including the virulence determinant ESX-1 secretion system40. Phylogenetic analysis suggests a slow initial expansion of pe/ppe genes, which accompanies the duplication of esx gene clusters, and that the pe-pgrs and ppe-mptr gene subfamilies are the most recent and expansive sublineage, sublineage V40. Interestingly, sublineage V is only present in the MTBC and closely related M. marinum and M. ulcerans, suggesting that it may play a role in host-pathogen interactions40. Within this context, it is striking to see that majorities of pe/ppe genes (79 out of 98) regulated by WhiB4 belong to sublineage V, including 67 pe-pgrs and 12 ppe-mptr genes (Supplementary Table S2). Furthermore, most of these pe-pgrs and ppe-mptr genes (55 out of 67) were positively regulated by WhiB4, including those that have been experimentally demonstrated to be critical for M. marinum virulence (e.g., pe-pgrs30, pe-pgrs62, and ppe38)43, 44, 57. Taken together, these data provide a plausible explanation for the essential role of WhiB4 in M. marinum virulence. We should point out that since the complemented strain was not included in the RNA-seq analysis, we cannot fully exclude the possibility that some differences in the gene expression could be linked to possible WhiB4-independent genetic modification(s) than may occur in a given selected transposon mutant.

At present it is not clear why WhiB4 did not perform a similar function in M. tb as in M. marinum, considering that a large number of pe-pgrs and ppe-mptr genes are also present in M. tb genome. Of the 98 pe/ppe genes regulated by WhiB4 in M. marinum, 59 genes have an ortholog in M. tb, 20 genes are restricted to M. marinum and 15 are present in both M. marinum and M. ulcerans (Supplementary Table S2). Previously, it was found that WhiB4 binds DNA non-specifically but has a preference for GC rich DNA sequences30. This is consistent with our finding that a large number (67) of pe-pgrs genes were regulated by WhiB4. The high glycine content (up to 50%) of PE-PGRS proteins makes their corresponding genes GC rich. On the other hand, the fact that there are still 81 pe-pgrs genes in M. marinum that are apparently not regulated by WhiB4 implies that cofactors may be required for WhiB4 mediated regulation of pe/ppe genes, and that these factors may be missing in M. tb. Several transcriptional regulators including sigma factors SigB and SigD, two-component systems PhoPR and MprAB have been shown to regulate specific pe/ppe genes (reviewed in ref. 42). However, compared to WhiB4, the number of pe/ppe genes controlled by these regulators is much lower. Whether these regulators interact with WhiB4 or whether additional factors are required for WhiB4 mediated regulation of pe/ppe genes will be addressed in future studies.

Methods

Bacterial strains and culture conditions

M. marinum strain 1218R (ATCC 927) and the transposon insertion mutant M. marinum ΔwhiB4 were routinely grown in Middlebrook 7H9 broth or 7H11 agar ((Difco™) supplemented with 10% OADC (oleic acid-albumin-dextrose-catalase) and 0.05% Tween80. Escherichia coli strain DH5α was used for routine manipulation and propagation of plasmid DNA. E. coli DH5α was grown in LB broth or agar. Antibiotics were added as required: kanamycin, 50 µg/ml for E. coli and 25 µg/ml for mycobacteria; hygromycin, 150 µg/ml for E. coli and 75 µg/ml for mycobacteria.

Isolation of transposon insertion whiB4 mutant

The mariner-based transposon system was used to generate a transposon insertion mutant library of M. marinum 1218R as described previously33, 58. Colonies with unusual morphology were identified by visual inspection. The method used to localize and identify the transposon-disrupted gene has been previously described33, 58. Briefly, total chromosomal DNA of the transposon insertion mutant was cleaved with BamHI, then self-ligated with T4 DNA ligase and transformed into competent E. coli DH5α λ pir116 cells. Plasmid DNA was isolated from kanamycin resistant E. coli transformants and MycoMar-specific primers were used to determine the DNA sequence at the transposon/chromosomal junction.

Molecular cloning

A DNA fragment containing intact whiB4 gene and 400 bp upstream of its start codon was amplified by PCR using the forward primer 5′-GCTACGCCAGCCCGTTAGGA-3′ and the reverse primer 5′-GCTTGAGCAGGGTGAGTGCG-3′. M. marinum genomic DNA was used as template. The fragment was ligated into pDRIVE and the resulting construct was isolated and digested with BamHI and HinDIII. The whiB4 containing fragment was ligated into pNBV1 which contains hygromycin resistance gene to generate pWhiB4. Standard electroporation protocols were used for transformation of pWhiB4 into M. marinum ΔwhiB4. Transformants were selected on Middlebrook 7H11 agar containing hygromycin (75 μg/ml) and kanamycin (25 μg/ml). Three randomly selected clones were tested.

Scanning electron microscopy analysis

Mycobacterial cultures (5 ml each) grown to mid-log phase were collected and added with two droplets of heparin sodium. The cultures were then fixed, dehydrated and observed using scanning electron microscope SU5000 (Hitachi, Japan).

Analysis of M. marinum intracellular growth

Infection of the marine RAW 264.7 macrophage cells and enumeration of intracellular M. marinum were performed as previously described59. Briefly, RAW cells were seeded into 24-well plates at a density of 105 cells per well in DMEM medium supplemented with 10% FBS for 24 hr. RAW cells were then infected with M. marinum at multiplicity of infection (MOI) of 10 for 2 hr at 30 °C in 5% CO2. Wells were washed twice with sterile PBS and extracellular M. marinum were killed by incubating the tissue culture with 200 μg/ml gentamycin for 2 hr. Cells were again washed twice with PBS and subsequently, incubated at 32 °C in 5% CO2 in fresh media with 20 μg/ml gentamycin. At different time points, the infected macrophage monolayers (3 wells per strain) were washed 3 times with fresh media and then lysed with 0.1 ml of 1% Triton X-100 to release intracellular mycobacteria. The number of intracellular mycobacteria was enumerated by plating appropriate dilutions on Middlebrook 7H10 agar plates containing appropriate antibiotics. Two independent experiments were performed.

Cytokine assay

RAW 264.7 macrophage cells were infected with mycobacteria at MOI of 10 as described above. At 3, 12, and 24-hr post infection, cell-free supernatants were collected and the levels of TNF were determined by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Dakewa, China). Two independent experiments were performed.

Ethics statement

All of the animal procedures were approved by the local animal care committees at Fudan University. All methods were performed in accordance with the relevant guidelines and regulations.

Zebrafish infection

Zebrafish infection with M. marinum was performed as previously described57. Briefly, adult zebrafish (15 per group) were infected by intraperitoneal injection with a dosage of 104 CFU bacteria per fish or PBS as the negative control and monitored for their survivals.

For histopathological study, two fish were sacrificed at the indicated time. Fish were fixed and then dehydrated with ethanol. After paraffin embedding and sectioning, serial paraffin sections were prepared and subjected to hematoxylin and eosin (H&E) staining and Ziehl-Neelsen acid fast staining. Sections were examined under a Nikon Eclipse Ni-U Microscope (Japan), and images were collected with a digital camera. Two independent zebrafish infection and survival experiments were performed.

RNA extraction and illumine sequencing

M. marinum WT and ΔwhiB4 each were grown in 5 duplicate cultures, each containing 5 ml 7H9 media to OD600 ~1.5. 1-ml cells from 5 duplicate cultures were collected and mixed together. The bacterial cultures were pelleted and washed twice with PBS. Total RNA (1–3 µg) was isolated using the RNeasy Mini Kit (Qiagen) and purified using the RNAClean XP Kit (Beckman Coulter) and RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions. Purified RNA was used to construct cDNA library according to the TruSeq Stranded RNA LT Guide from Illumina. The concentration and size distribution of cDNA library was analyzed by Agilent 2100 Bioanalyzer and the average library size was approximately 350 bp. High-throughput sequencing was carried out on an Illumina HiSeq 2500 system according to the manufacturer’s instructions (Illumina HiSeq 2500 User Guide) and 150-bp paired-end reads were obtained. The raw reads were filtered by Seqtk and then mapped to the M. marinum M strain reference sequence (GenBank GCA_000018345.1) using Bowtie2 (version: 2–2.0.5)60. Counting of reads per gene was performed using HTSeq followed by TMM (trimmed mean of M-values) normalization61, 62. Differentially expressed genes were defined as those with a false discovery rate <0.05 and fold-change >2 using the edgeR software63. Two independent experiments were performed.

Statistical analysis

Two-Way ANOVA were performed on data of 3 groups at multiple time points (macrophage infection experiments). Zebrafish survival curves were plotted using the Kaplan-Meier method and differences between curves were analyzed using the log-rank test.