Propagation rate of phage clones. 106 each of the four phage clones were individually added into the logarithmic growth phase culture of ER2738 for infection and amplification for 5 hours at 37 °C. Titer of each clone was quantified every 1 hour by counting plaques on LB/IPTG/X-gal plates. PB-TUP did not show propagation advantage compared with the other phage clones. M13KE phages had a faster growth rate than the other clones.