Figure 7 | Scientific Reports

Figure 7

From: Neonicotinoid-induced pathogen susceptibility is mitigated by Lactobacillus plantarum immune stimulation in a Drosophila melanogaster model

Figure 7

Lp39 supplementation mitigates IMI-induced D. melanogaster survival deficits following infection. (A) Survival curves for newly eclosed WT Canton-S flies that were subjected to septic infection with Serratia marcescens NCIMB 11782 while concurrently exposed to IMI and/or supplemented with Lp39 separately or in combination, compared to infected WT-Canton control and vehicle (septic injury alone). All statistical symbols are representative of comparison made to infected WT Canton-S controls. Statistical significance was determined using the log-rank (Mantel-Cox) test. (B) Dpt expression of newly eclosed Dpt-RFP reporter flies was determined following 15 h oral supplementation of Lp39 or vehicle. WT Canton-S flies were used as a negative control. Gene expression was quantified by fluorescence intensity using a microplate reader. Mean ± standard deviations (two-way ANOVA) from 7 biological replicates (10 flies each replicate) per group are shown. (C) Newly enclosed Dpt-RFP and negative control WT Canton-S flies were orally supplemented with Lp39 or vehicle for 15 h. Midguts were dissected, nuclear counterstained with Hoechst 33342, trihydrochloride, tetrahydrate and viewed by confocal microscopy. Scale bar = 20 µm. **p < 0.01, ***p < 0.001, ****p < 0.0001.

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