Table 4 Polymorphisms in pfK13.

From: High throughput resistance profiling of Plasmodium falciparum infections based on custom dual indexing and Illumina next generation sequencing-technology

Base position Codon position Reference base Alternative base Reference codon Alternative codon Reference freq. Alternative freq. Mixed freq.
Non-synonymous K13 polymorphisms
65 22 G A R K 99.7% 0.3% 0.0%
252 84 A G L K 99.7% 0.3%  
335 112 G A G E 99.7% 0.9% 0.6%
340 114 A C L Q 99.1% 1.3% 0.3%
445 149 A T T S 99.4% 0.6%  
566 189 A C L T 53.8% 57.9% 11.6%
567 189 A T L N 98.1% 3.5% 1.6%
820 274 C T H Y 99.7% 0.3%  
1732 578 G T A S 99.1% 1.6% 0.6%
2092 698 T A F I 99.7% 0.3%  
Synonymous K13 polymorphisms
57 19 G A T T 99.7% 0.3%  
321 107 T C S S 99.7% 0.3%  
417 139 T C N N 99.4% 0.9% 0.3%
1407 469 C T C C 99.1% 1.9% 0.9%
1434 478 C G T T 99.7% 0.3%  
1881 627 T A A A 99.7% 0.3%  
2070 690 C A G G 99.7% 0.3%  
  1. The table describes the various polymorphisms found in pfK13 for the 318 resistance-profiled samples, and the frequencies in which the reference and alternative base were found, as well as the frequencies of mixed infections. SNP positions written in italic indicate that they are located in the propeller region of pfK13. Note that mixed infections were counted in both the reference and alternative frequencies.