Figure 2 | Scientific Reports

Figure 2

From: Substrate Rigidity Controls Activation and Durotaxis in Pancreatic Stellate Cells

Figure 2

Soft matrices induce PSC deactivation. (a) Bright-field images of Oil Red O stained PSCs on glass, soft and stiff matrices for 6 days. Scale bar 25 μm. (b) Cell population Oil Red staining levels after 3 days on soft or stiff PAA matrices show that active PSCs on soft matrix begin to revert back to quiescence. Representative of 3 independent experiments with 33 cells analysed. (c) Cell population Oil Red O staining levels after 3, 6 and 9 days on soft matrix confirm that culture of active PSCs on soft matrices reverts cells back into a resting state in a time-dependent manner. (d,e) pPCR mRNA levels of aSMA and vimentin for conditions represented in (a). (f,g) Quantification of staining intensity for αSMA and vimentin for conditions represented in (a), images in Supplementary Fig. 5. In all cases, histogram bars represent mean ± SEM. Data are representative of 3 independent experiments and 221 cells analysed in (c), *p < 0.05, **p < 0.01, ***p < 0.001, n.s. non-significant.