Figure 1 | Scientific Reports

Figure 1

From: Substrate Rigidity Controls Activation and Durotaxis in Pancreatic Stellate Cells

Figure 1

Stiff matrices induce PSC activation. (a) Bright-field images of Oil Red O stained PSCs on Matrigel, soft and stiff matrices for 6 days. Scale bar 25 μm. (b) Quantification of Oil Red O staining after 24-hour culture on soft or stiff PAA matrices showed a significant reduction in staining levels on stiff matrix when compared to soft matrix, indicating cellular activation almost entirely on stiff matrix rigidities. (c,d) pPCR mRNA levels of αSMA and vimentin for conditions represented in (a). (e,f) Quantification of staining intensity for αSMA and vimentin for conditions represented in (a), images in Supplementary Fig. 3. In all cases, histogram bars represent mean ± SEM. Representative of 3 independent experiments with more than 20 cells analysed in (b,e,f), ***p < 0.001, **p < 0.01.