Figure 5 | Scientific Reports

Figure 5

From: FAM3B mediates high glucose-induced vascular smooth muscle cell proliferation and migration via inhibition of miR-322-5p

Figure 5

Overexpression of FAM3B inhibits miR-322-5p expression. (A) Microarray analysis of miRNA expression in VSMCs infected with adenoviruses expressing either EGFP or FAM3B (left). RT-qPCR validation of miRNAs potentially downregulated by FAM3B (right). * P < 0.05 and ** P < 0.01 vs. Ad-EGFP. (B) Analysis of the transcriptional activity of pre-miR-322 promoter by reporter gene assays. * P < 0.05 vs. vehicle. (C) RT-qPCR analysis of miR-322-5p expression in VSMCs stimulated with various concentrations of glucose for 36 h. * P < 0.05 and ** P < 0.01 vs. 5.5 mM glucose. (D) RT-qPCR analysis of miR-322-5p expression in VSMCs stimulated with 25 mM glucose for the indicated time-points. * P < 0.05 and ** P < 0.01 vs. 0 h. (E) RT-qPCR analysis of miR-322-5p expression in VSMCs with FAM3B knockdown. ** P < 0.01 vs. scra/5.5 mM glucose, ## P < 0.01 vs. scra/25 mM glucose.

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