Expression profiles of cholesterol metabolism-related genes are altered during development of experimental autoimmune encephalomyelitis in the rat spinal cord

Increased evidence suggests that dysregulation of cholesterol metabolism may be a key event contributing to progression of multiple sclerosis (MS). Using an experimental autoimmune encephalomyelitis (EAE) model of MS we revealed specific changes in the mRNA and protein expression of key molecules involved in the maintaining of cholesterol homeostasis in the rat spinal cord: 3-hydroxy-3-methylglutaryl-coenzyme-A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24-hydroxylase (CYP46A1) during the course of disease. The presence of myelin lipid debris was seen only at the peak of EAE in demyelination loci being efficiently removed during the recovery period. Since CYP46A1 is responsible for removal of cholesterol excess, we performed a detailed profiling of CYP46A1 expression and revealed regional and temporal specificities in its distribution. Double immunofluorescence staining demonstrated CYP46A1 localization with neurons, infiltrated macrophages, microglia and astrocytes in the areas of demyelination, suggesting that these cells play a role in cholesterol turnover in EAE. We propose that alterations in the regulation of cholesterol metabolism at the onset and peak of EAE may add to the progression of disease, while during the recovery period may have beneficial effects contributing to the regeneration of myelin sheath and restoration of neuronal function.

The progression of EAE alters expression profiles of HMGCR, АpoЕ and CYP46A1 in the spinal cord. Accumulating evidence underscores the capability of perturbed cholesterol homeostasis to modulate inflammation and potentially promotes autoimmune disease 27,28 . Therefore, we next analyzed the expression pattern of key molecules involved in biosynthesis (HMGCR), recycling (ApoE), and degradation (CYP46A1) of cholesterol in the course of EAE using real-time PCR and Western blot analysis (Fig. 2).
At the onset of disease there were no significant changes in HMGCR mRNA level compared to control. Interestingly, although the levels of HMGCR mRNA was significantly reduced (~7 fold) at the peak of disease, it was returned to control level at the end of disease ( Fig. 2A). Correspondingly, the prominent decrease of the HMGCR at the protein level was only detected at the peak of EAE (Fig. 2B).
ApoE mRNA levels was significantly decreased at the onset, while at the peak and the end of EAE it returned to control levels (Fig. 2C). In contrast, ApoE protein level exerts the most pronounced changes at the peak of disease. ApoE protein expression was significantly increased compared to the levels in controls and at the onset of disease and remained elevated throughout the recovery period until the end of disease (Fig. 2D).
CYP46A1 expression profile revealed a significant decrease in mRNA levels at all investigated time points, being at the lowest level at the peak of disease (Fig. 2E). Particularly, when compared to control, CYP46A1 mRNA levels decreased 2-, 5-and 2-fold at the onset, peak and the end of EAE, respectively. In contrast, CYP46A1 protein levels remained unchanged during the course of disease (Fig. 2F).

CYP46A1 expression localizes in the areas of demyelination during EAE.
To visualize demyelination process during the course of disease, spinal cords from EAE animals were stained with Sudan black and compared with controls. At the onset of EAE (Fig. 3D), as well as in the control sections (Fig. 3A) myelin was compact and stained black. At the subsequent time points, pale blue staining of demyelination loci was observed in the dorsal funiculus, preferentially in the gracile fasciculus (Fig. 3G,J).
In order to detect the presence of lipid debris during the development of EAE consecutive sections were stained with Oil-Red-O (Fig. 3B,E,H,K). Myelin lipid debris was seen only at the peak of EAE ( Fig. 3H and insets) and coincided with the areas of increased myelin pallor (Fig. 3G). The absence of lipid laden myelin debris at the end of EAE (Fig. 3K) suggests their efficient clearance during the recovery period.
Given the important role of CYP46A1 in the clearance of cholesterol, the main lipid constituent of the membranes, we next performed histological examination of CYP46A1 staining (Fig. 3C,F,I,L) and revealed its presence in the areas of demyelination and lipid degradation. The most prominent CYP46A1 expression was detected at the peak of disease and was associated with the plaques of demyelination in the gracile fasciculus (Fig. 3I). Interestingly, further inspection of CYP46A1 immunoexpression revealed its localization with different types of cells in the spinal cord sections of all experimental groups ( Supplementary Fig. 2). In the white matter (dorsal, lateral and ventral) CYP46A1 immunoreactivity was mostly associated with the glia-like cells  Fig. 2I-L). Next, to confirm CYP46A1 co-localization with particular type of cells and to reveal whether it is altered during the course of disease, we performed double immunofluorescence staining with specific cell type markers. CYP46A1 expression localizes in macrophages/microglia infiltration during the progression of EAE. CYP46A1 presence in the macrophages/microglia over the course of EAE was demonstrated by CYP46A1/ED1 double immunofluorescence, using ED1 as a marker of macrophages and reactive microglia. In the control spinal cord sections no CYP46A1/ED1 immunoreactivity was detected (data not shown). At the onset of disease, ED-1 immunopositive cells were primarily observed in a close proximity to the pial surface of the spinal cord and in perivascular space scattered between CYP46A1 positive structures predominantly in dorsal funiculus of spinal cord ( Fig. 4A-C). In ventral and lateral regions of the spinal cords ( Supplementary Fig. 3A-F), CYP46A1 expression in ED1 + macrophages was detected exclusively in the areas of infiltration. During the peak of disease, numerous amoeboid cells were ED1 + . Clusters of round ED1 + cells were observed throughout the spinal cord parenchyma, especially at gracile fasciculus in the area of demyelination (Fig. 4D). Strong CYP46A1 staining (Fig. 4E) has been shown to localize in ED1 + macrophages within the areas of macrophage infiltration in spinal cord tissue (Fig. 4F). Similarly, in the ventral and lateral regions of spinal cords, CYP46A1/ED1 immunoreactivity overlaps with macrophage infiltrates (Supplementary Fig. 3G-L). At the end of disease, sparse ED1 + cells were dispersed among CYP46A1 positive structures in dorsal funiculus ( CYP46A1 is expressed by resident microglia at the peak and the end of EAE. To reveal the presence of CYP46A1 in the resident microglia the spinal cord sections were stained with Iba1, which is expressed both in resting and reactive microglia. In the control sections, ramified Iba1 and CYP46A1 positive cells were sporadically present in dorsal and ventral funiculus (  . Disease severity scores. EAE was induced in female Dark Agouti rats following immunization with rat spinal cord homogenate (50% w/v emulsion in saline) emulsified in complete Freund's adjuvant. Naïve rats served as a control. Animals were scored for neurological signs of EAE according to the standard 0-5 EAE grading scale (black line): 0 -unaffected; 1 -tail atony; 2 -hind limb weakness; 3 -complete hind limb paralysis; 4 -tetraplegic; 5 -moribund state or death. Progress of EAE was associated with body weight loss, with maximal loss that coincided with paralysis at the peak of disease (red line). Animals from EAE group (10/group) were sacrificed at the onset (D9), peak (D13) and end (D22) of disease. Data are expressed as the mean clinical score ± SEM (10 rats per group). Differences between the experimental groups were tested using ANOVA followed by Bonferroni test. # Peak vs. onset of EAE, $ Peak vs. end of EAEs (P < 0.05).  Fig. 5G-L). However, CYP46A1staining in GFAP + astrocytes in the gray matter could not be observed (Fig. 7B). The most prominent phenotypic changes of astrocytes occurred at the peak of disease adjacent to the area of demyelination. Hypertrophied astrocytes with enlarged intertwining processes formed the scar border (Fig. 6G, Supplementary Fig. 5M,P). Densely populated GFAP + astrocytes expressing CYP46A1 were seen in dorsal funiculus (Fig. 6I). Similarly, CYP46A1 + /GFAP + hypertrophied astrocytes with short processes comprised ventral and lateral regions of the spinal cord ( Supplementary Fig. 5O,R). In the gray matter, intensive CYP46A1 staining was present in GFAP + reactive astrocytes (Fig. 7C,D). At the end of EAE, hypertrophied astrocytes with elongated processes expressing CYP46A1 were observed in the gracile fasciculus ( Fig. 6J-L). HMGCR, (C) ApoE and (E) CYP46A1 mRNAs isolated from lumbar part of spinal cords of control and EAE rats at the onset, peak and the end of disease were assessed by Real-time PCR. β-actin was used as an endogenous control. The protein levels of (B) HMGCR, (D) ApoE and (F) CYP46A1 were measured by Western blots. Shown are representative blots for each protein with β-actin used as a loading control. Both mRNA and protein levels are expressed as a fold change relative to the values in spinal cords of control animals. The data represent the mean ± SD.*P < 0.05 vs. control; # P < 0.05 vs. onset; $ P < 0.05 vs. end of EAE. Decreased neuronal expression of CYP46A1 coincides with the peak of EAE. Double immunolabeling of spinal cord sections with NeuN, as a marker of neuronal nuclei, revealed a clear staining for CYP46A1 ( Fig. 8E-H). Prominent CYP46A1 immunoreactivity in neuronal somata was observed in the gray matter of all examined groups (Fig. 8A,B,D), except at the peak of disease when intensity of CYP46A1 staining was lowered (Fig. 8C).
In the white matter, double immunofluorescence with anti-NF200 antibody revealed the presence of CYP46A1 immunoreactivity in the axons during the course of disease ( Fig. 8M-P) and confirmed that decrease in CYP46A1 staining at the peak and, to a lesser extent, at the end of disease (Fig. 8K,L) is due to axonal loss ( Fig. 8O,P).

Discussion
In the present study, using experimental autoimmune encephalomyelitis (EAE) as an animal model for MS, we found a link between the expression pattern of key molecules involved in the biosynthesis (HMGCR), recycling (ApoE) and degradation (CYP46A1) of cholesterol and the progression and resolution of EAE in the rat spinal cord. This implies that maintaining a balance between cholesterol utilization and excretion is very important during EAE. In addition, we have shown that aside from its expression in neurons, CYP46A1 was localized within different types of glial cells in a time-dependent manner.
Numerous studies over the past decades have reported associations between MS disease outcome and levels of cholesterol precursors, oxysterols and ApoE in circulation and CSF 8,9,30 , indicating the need for closer insight into the cholesterol homeostasis in MS pathogenesis. However, to the best of our knowledge, the cholesterol homeostasis in the spinal cords during EAE has been poorly addressed so far.
Given that HMGCR is a rate limiting enzyme in the cholesterol biosynthesis in this study we examined the relationship between its gene and protein expression and the clinical scores of EAE. We have observed that the most prominent down-regulation of HMGCR gene and protein expression occurred in the spinal cord at the peak of disease, when inflammation and demyelination are the most pronounced. Similarly, reduced HMGCR gene expression was detected at the peak of MOG-induced EAE in female DA rats 31 as well as in lysolecithin model of demyelination 32 . Recently, it was shown that products of the cholesterol biosynthesis pathways are interrelated with myelin genes expression 33 . Pertinent to the later, the observed suppression of HMGCR expression may be correlated with persistent down-regulation of transcripts for several myelin proteins like MOG, MBP, MAG, during EAE 31 . Furthermore, alteration in HMGCR expression is associated with misdirected OPC migration, leading to hypomyelination 33 . Since HMGCR expression is negatively regulated by cholesterol content, the lower expression of both HMGCR transcript and protein detected at the peak of EAE may be the consequence of high cholesterol concentration due to demyelination in spinal cords of EAE animals, reducing the need for cholesterol biosynthesis. In contrast, at the end of disease, the observed increase in HMGCR expression is probably due to the increased demand for cholesterol needed for re-myelination during the recovery period.
ApoE plays a central role in lipid transport and cholesterol metabolism in the CNS. It exerts pleiotropic effects during EAE, both protective 34,35 and harmful 36,37 . Here, we have observed the significant down-regulation of ApoE expression at the onset of disease, followed by elevated levels at the peak and the end of disease. It is plausible to presume that elevation of ApoE levels at the peak and the end of disease may be due to the increased number of glial cells that we have detected in the spinal cord of EAE animals, particularly in and around the areas of demyelination. Our results are in agreement with Shin et al. 36 , who also observed elevated expression of ApoE during EAE and suggested that invading cells may be responsible for part or all of the enhanced ApoE. In line with this, in active MS lesion, the increased ApoE expression was found in astrocytes and macrophages 38 , supporting the ApoE involvement in lipid trafficking needed for neuronal repair 39,40 . Indeed, it was shown that cholesterol released as a result of demyelination associates with ApoE-containing lipid particles and is available for reutilization 41 . In addition, ApoE acts to remove cholesterol from damaged neurons in order to restore axonal properties, with concomitant reduction of inflammation 9 . Furthermore, Apo E deficiency leads to massive infiltration within CNS during EAE 35 and increased permeability of BBB 42 , indicating that ApoE has strong anti-inflammatory effect on EAE progression 34 . Indeed, elevated expression of ApoE that we have observed at the end of EAE may be direct response to demyelination, as it is proposed by Schrewe et al. 43 .
CYP46A1 is the major regulator of CNS cholesterol elimination 44 . So far, no studies have been carried out to evaluate possible correlations between the CYP46A1 mRNA and protein content in the rat spinal cord and onset, progression and resolution of EAE. In this study, we have observed reduction of CYP46A1 mRNA levels at the onset of disease, which was followed by further decrease at the peak of EAE. However, this drop in the CYP46A1 mRNA expression was returned to the control level during the period of recovery. Notably, protein content of CYP46A1 remains unchanged during the course of the disease. Given that CYP46A1 is predominantly produced by neurons, the observed decrease in CYP46A1 mRNA possibly reflects the loss of neuronal cells which is the most pronounced at the peak of EAE. On the other hand, unchanged protein content points that other type of cells, presumably glial cells, contribute to CYP46A1 production. Indeed, we have shown the presence of CYP46A1 in infiltrated macrophages, microglia and astrocytes in the areas of prominent demyelination in the course of EAE. Similarly, it was shown that decrease of CYP46A1 in neuronal cells is at least in part compensated by an induction of the enzyme in glial cells 24 . In addition, Teunissen et al. 45 proposed that infiltrating macrophages participated in CYP46A1 production during EAE. During EAE, activated microglia and infiltrated macrophages are involved in the clearance of myelin debris and apoptotic cells from the CNS. Moreover, myelin debris removal is prerequisite for remyelination process. In our study, the appearance of lipids and lipoproteins as a part of destroyed myelin was detected at the peak of disease as confirmed by Oil Red-O staining. The absence of lipid components at the end of disease implies that the remains of degraded myelin were efficiently removed by activated microglia. Interestingly, we have demonstrated heightened CYP46A1 expression within the areas of demyelination during EAE. Furthermore, aside from neurons CYP46A1 immunoreactivity can be observed in infiltrating and resident macrophages/microglia and astrocytes.
Previously, we and others have shown that activated macrophages/microglia express CYP46A1 in traumatic brain injury 26,46 . Additionally, it was suggested that infiltrating macrophages express CYP46A1 during EAE 45 . Although it is difficult to distinguish activated resident microglia from infiltrating macrophages, a strong correlation between monocyte infiltration and progression to the paralytic stage of EAE suggests that the infiltrating macrophages are crucial for the pathogenesis 47 . Activated microglia can occur at early stage of tissue injury and usually precedes the formation of demyelinated plaques, which contribute to progressive neurodegeneration 48 . Our study confirmed the presence of CYP46A1 in infiltrated macrophages during development of EAE and further demonstrated its region-specific pattern of expression in spinal cords. The strongest CYP46A1 staining in activated macrophages/microglia was found at the peak of disease in the areas of demyelination. During recovery process, the population of infiltrating monocytes vanishes, while activated microglia enters the cell cycle and returns to quiescence 2 . Indeed, in our study microglia acquire resting form in recovery phase, with some cells still expressing CYP46A1.
The role of astrocytes in MS pathogenesis depends on various factors, including: the stage of the disease, the environment surrounding the lesion, and the interactions with other cell types, which may affect their state of activation 6 . During MS or EAE, astrocytes undergo proliferation and extensive hypertrophy of cell bodies, accompanied by thickening and elongation of their processes 49 . Activated astrocytes in EAE and MS are associated with the glial scar formation, where a physical border composed of hypertrophic astrocytes separates an area of CNS damage from normal appearing tissue 50 , resulting in the extracellular matrix composition changes, which prevent remyelination 51 . It was proposed that the availability of cholesterol is crucial for myelin membrane growth 52 and astrocytes have been described as an important source of cholesterol for neurons 53 . Previously, we have shown an increase of GFAP gene and protein expression at the end of EAE, suggesting astrocytes involvement in neuroprotection 50 and possibly in lipid trafficking 54 . The novel finding of this study is the association of CYP46A1 with reactive astrocytes surrounding demyelination plaques in the spinal cord of EAE rats, where those astrocytes are probably involved in clearance of axonal and myelin debris. Namely, immunofluorescence analysis revealed that radially-oriented GFAP + astrocytes expressed CYP46A1 throughout the white matter of spinal cords during EAE. In the grey matter, besides obvious CYP46A1 expression in neuronal cell bodies, the abundant presence of this enzyme can be noticed in hypertrophic astrocytes at the peak and the end of disease. It is supposed that activated astrocytes in recovery phase of EAE have a putative role in reducing inflammation and in protection of the neurons from oxidative stress 55 . Thus, the apparent localization of CYP46A1 in astrocytes at the end of disease, either in white or gray matter, possibly correlates with recovery process and remyelination. Those processes have high demand for cholesterol synthesis and CYP46A1 is needed in order to remove excess of cholesterol that fails to incorporate into the myelin membranes.
CYP46A1 expression was found in many types of neurons, where a majority of CYP46A1 expression was exclusively associated to neuronal somata, with lower levels found in dendrites 23,26,44,56 . This study also revealed a strong CYP46A1 immunoreactivity in the neuronal somata in the grey matter at the onset and the end of EAE, which was lowered at the peak of disease probably due to neuronal degeneration. For the first time we have been shown the presence of CYP46A1 in axons. It is important to note pronounced axonal CYP46A1 immunoreactivity at the onset and the end of EAE and diminished immunoreactivity at the peak of the disease. This indicates that changes in pattern of CYP46A1 expression may reflect the degree of axonal damage. Up to now, positive CYP46A1 immunostaining of axons in the white matter was only seen in the normal human brain and in the brains of Alzheimer's disease patients 24 . The authors proposed that CYP46A1 may be transported from the neuronal cell bodies to the periphery, suggesting that such mechanism may be of importance for maintaining the plasticity of the synapses. This assumption was recently confirmed by Moutinho et al. 13 , who report that CYP46A1 promotes neuronal outgrowth and increases synaptic markers.
Summarizing, the observed data indicate that alterations in the regulation of cholesterol metabolism during the onset and peak of EAE may add to the progression of disease, while during the recovery period may have the beneficial effects contributing to the regeneration of myelin sheaths and restoration of neuronal function. It appeared that gene and protein expression of key regulators of cholesterol homeostasis HMGCR, ApoE and CYP46A1 correlates with the clinical scores of disease. Cellular profiling of CYP46A1 expression in the spinal cord during EAE revealed that its expression is not exclusively in neuronal cells. The presence of CYP46A1 was confirmed in infiltrated macrophages, microglia and astrocytes, indicating that these cells in neuroinflammatory environment become sensitive to excess of cholesterol and play a role in cholesterol turnover. The fact that cholesterol is a crucial, rate-limiting factor for myelin growth and that its metabolites may have a role in promoting autoimmunity as inflammatory mediators, warrants further investigation of cholesterol metabolism.

Materials and Methods
Experimental animals, EAE induction and disease severity assessment. All experiments were conducted using two-month-old female rats of Dark Agouti inbred strain from the local colony. Animal procedures were approved by Ethical Committee for the Use of Laboratory Animals of Institute for Biological Research "Sinisa Stankovic" (Belgrade, Serbia), as being in compliance with EEC Directive (86/609/EEC) on the protection of animals used for experimental and other scientific purposes. Animals were housed (3-5/cage) in a pathogen-free and climate-controlled environment in polystyrene cages containing wood shavings with free access to standard rodent chow and water with regulated 12-hour light/dark cycles. During period of paralysis, animals were watered manually.
Animals were randomly divided into two groups (control, n = 10 and EAE group, n = 30). EAE was induced with the spinal cord homogenate (prepared from freshly dissected spinal cords) and mixed with an equal volume of complete Freund's adjuvant containing 0.5 mg/ml Mycobacterium tuberculosis (CFA; Sigma, St. Louis, MO, USA) in a 1:1 ratio. Female Dark Agouti rats (150-200 g) were inoculated with a 100 μl intradermal injection of the prepared emulsion. Immunization was performed under ether anesthesia. The group of age-matched intact animals was used as a control.
Tissue preparation. Rats were deeply anesthetized with Zoletil ® 50 (Virbac, France; 30 mg/kg i.p.). Animals from EAE group were sacrificed at the onset (9 dpi, D9), peak (13 dpi, D13) and end (22 dpi, D22) of disease. Age-matched naïve animals were used as control. Lumbar regions of the spinal cords were used for all further tissue processing.
Real-time PCR. Lumbar spinal cord segments were collected at D9, D13 and D22 (3/group). In order to minimize degradation of spinal cord samples, anesthetized rats were perfused intracardially with 50 ml of cold (4 °C) saline. Afterwards, the rats were placed on ice, and a laminectomy of the entire lumbar spinal cord was performed. The excised segments were placed in precooled (4 °C) sterile RNase/DNase-free microcentrifuge tubes and immediately flash frozen in liquid nitrogen and kept at −80 °C until RNA extraction. Total RNA was isolated using Trizol Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA). The RNA concentration was assessed by determining OD260, whereas the purity was evaluated based on OD260/OD280 ratio (1.8-2.1, for all samples) and gel inspection. RNA was reverse transcribed using High-capacity cDNA RT-kit (Applied Biosystems, Carlsbad, CA, USA). TaqMan PCR reactions were performed with Assay on-Demand Gene Expression Products (Applied Biosystems, Carlsbad, CA, USA) for ApoE (Assay ID (assay ID Rn00593680_m1) and HMGCR (Assay ID assay ID Rn00565598_m1), and assay-by design gene expression product for CYP46A1, with the following sequences: forward primer (50-30) CAGCT TCCTTCTGGGACATCTC; reverse primer (50-30) GAGCACACGGCCACAAG and FAM-MGB probe  TTCGTCCTTTTTCCAAAAGT. Reactions were performed in a 25-μl reaction mixture containing 1 × TaqMan Universal Master Mix (Applied Biosystems, Carlsbad, CA, USA) and the cDNA template (10 ng of RNA converted to cDNA). PCR reactions were carried out in the ABI Prism 7000 Sequence Detection System (Applied Biosystems) at 50 °C for 2 min, at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and at 60 °C for 1 min. The experimental threshold was calculated from the mean baseline fluorescence signal from cycles 3 to 15, plus 10 standard deviations. Each sample was run in triplicate and a mean value of each Ct triplicate was used for further calculation. An endogenous control was included in every analysis to correct the differences in inter-assay amplification efficiency, and the expression of each gene was normalized to actin expression. The obtained results were analyzed by RQ Study Add ON software for 7000 v 1.1 SDS instrument (ABI Prism Sequence Detection System) with a confidence level of 95% (P < 0.05).

Sudan black.
In order to analyze demyelination process during disease development, frozen sections were kept at room temperature, rinsed with propylene glycol, and stained with 0.7% Sudan black B (Sigma-Aldrich, St. Louis, MO, USA) diluted in propylene glycol, for 10 min. The specimens were rinsed with distilled water and mounted in Mowiol (Calbiochem, Millipore, Germany). Normal myelin appeared dark blue to black, while degraded myelin was light blue.
Oil red O staining. To stain lipids in frozen sections, they were washed in distilled water, rinsed in 60% propylene glycol with subsequent incubation in Oil red O for 15 minutes. Afterwards, the sections were washed in 60% propylene glycol, rinsed with distilled water and mounted in Mowiol (Calbiochem, Millipore, Germany).
Immunostaining. For immunohistochemical and immunofluorescence analyses spinal cords (4/group) were rapidly dissected on ice and fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (PBS), pH 7.4 for 12 hours at 4 °C. To cryoprotect lumbar regions of spinal cord tissue were immersed into the graded sucrose solutions (10-30% in 0.1 M PBS, pH 7.4). Afterwards, the spinal cords were embedded in OCT in 2-methyl butane (tissue freezing medium) for cryosectioning and stored at −80 °C. Serially cut, coronal sections (20 µm thick) were collected, mounted onto superfrost glass slides, allow drying for 2 h at room temperature and stored at −20 °C until staining. 20 µm thick transverse sections of the lumbar spinal cord (L1-L5) were cut and