Figure 1 | Scientific Reports

Figure 1

From: Microbiota modulation counteracts Alzheimer’s disease progression influencing neuronal proteolysis and gut hormones plasma levels

Figure 1

SLAB51 ameliorates behavioral performance and reduces brain damage in AD mice. Novel Object Recognition (NOR) test (first experiment): 15 mice/sub-group were allowed to explore an identical pair of objects, and after 3 hours, they are presented with the familiar object and a novel object. The discrimination scores for 8, 12, 18, and 24-week-old AD mice are reported in panel A. NOR test (second experiment) performed for the first time on treated and untreated 24-week-old mice (groups’ size = 10), panel B. Panel C: Elevated plus maze test. % open arm entries and % time spent in the open arms by untreated and treated AD mice at 24 weeks of age (first experiment, groups’ size = 15). Data points marked with an asterisk are statistically significant compared to their respective non-treated control mice (*p < 0.05). Panel D Immunodetection of FGF9 protein in brain slides of 24-weeks old untreated and treated wt and AD mice (8 animals per group) from the first experiment. Results are reported as number of cells immunoistochemically positive for FGF9 per field ± ES (#statistically significant with respect to the corresponding untreated mice p < 0.05). For each histological section 5 randomly selected field were analyzed at 40xHPFs. Representative images of immunohistochemical staining are reported. Panel E: Brain weights expressed in grams ± ES of both treated and untreated wt and AD mice over time (groups’ size is 8). Panel F: Measurement of the cortex thickness (mm) and ventricular sizes evaluation (mm3) in the brain sections of control and SLAB51 treated AD mice at 8, 12, 18 and 24 weeks of age. Data are reported as mean values ± ES (*statistically significant with respect to 8 weeks-old untreated mice p < 0.05; #statistically significant with respect to the corresponding untreated mice p < 0.05). Consecutive brain slides of treated and untreated 24 weeks-old AD mice are shown.