Figure 8 | Scientific Reports

Figure 8

From: A-to-I editing in human miRNAs is enriched in seed sequence, influenced by sequence contexts and significantly hypoedited in glioblastoma multiforme

Figure 8

Increased expression of mature edited form of hsa-miR-411. (A) The figure shows the predicted secondary structures for the unedited (I) and edited (II) versions of pre-mir-411 using RNAfold Webserver. The arrow shows the position of editing found experimentally in mature miR-411–5p. The edited version (A changed to G) was predicted to have a more stable structure (ΔΔG = −6.6 kcal/mol). (B) qRT-PCR data shows altered expression upon editing. A significant (two-tailed t-test, p = 0.001) increase in expression (3.5 fold, average of three replicates) was observed only for the mature form of miR-411. No significant (two-tailed t-test, p = 0.41) change was observed at the level of pri- and pre-mirna.

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