Validation of hypoediting and downregulation of mirna in GBM. (A) The X- and Y-axes represents the relative size of the SnaPshot product and relative fluorescence unit (RFU) respectively. One representative example of hypoedited miRNA (mir-1301) in GBM is shown. The editing event observed in normal brain RNA (I) is absent in the GBM tumor RNA (II) and in the normal genomic DNA (III). The SnaPshot specific primer was chosen from the negative strand, hence A to I (G) editing is depicted as T and C peaks. (B) Real time PCR validation of downregulation for hypoedited miRNAs in GBM samples compared to CC samples. Y-axis shows ΔCt values of miRNAs in five CC and five GBM samples and U6 snRNA expression was used for normalization. Statistical significance of downregulation was determined by one-tailed t-test. The delta Ct values for these four miRNAs are provided in Supplemental Table S7.