Diagnosing congenital malaria in a high-transmission setting: clinical relevance and usefulness of P. falciparum HRP2-based testing

Congenital malaria diagnosis is challenging due to frequently observed low parasite density infections, while their clinical relevance during early infancy is not well characterized. In Nanoro health district (Burkina Faso), we determined the prevalence of congenital malaria by real-time quantitative PCR and we assessed the performance of rapid diagnosis test (RDT) and light microscopy (LM) to detect Plasmodium falciparum infections in cord-blood samples. In addition, we examined the usefulness of P. falciparum Histidine Rich Protein2 (PfHRP2) as surrogate biomarker of infection and explored association between congenital malaria and clinical outcomes. A prevalence of congenital malaria by qPCR of 4% (16/400) was found, which increased to 10% among newborns from mothers infected at delivery. RDT and LM showed poor performances indicating limited utility for congenital malaria screening in cord blood. Because PfHRP2 detection in cord blood could be affected by transplacental passage of parasite antigens, PfHRP2 might not be used as a surrogate biomarker of congenital malaria infections. There was no evidence of a significant clinical impact of congenital malaria on infant’s health from birth to 59 days of life. Case control studies including long-term follow up may provide additional understanding on the relevance of neonatal malaria infections.

Congenital malaria is defined as the presence of Plasmodium asexual stages in newborn's cord or peripheral blood during the first week of life as a result of materno-fetal transfer of malaria parasites 1 . The reported prevalence of congenital malaria in sub-Saharan Africa ranges from 0% to 54.2% depending on geographic area, endemicity, study design and detection method (i.e. light microscopy -LM-or Polymerase Chain Reaction -PCR-) [2][3][4][5] .
In malaria-endemic areas, symptomatic neonatal malaria infections are rare, suggesting spontaneous clearance of parasites detected at birth 1, 6-8 . However, some newborns with congenital malaria can develop symptoms within days [9][10][11][12][13][14] , weeks or months after birth [15][16][17] . Even though consequences of congenital malaria may be life-threatening little is known about the impact of these infections on the infant's health, especially during the neonatal period (from birth to 28 days) 2,13,18,19 . Targeted diagnosis of high risk newborns e.g. those born from infected mothers, could inform neonatal clinical care and guide prompt malaria treatment in clinically relevant cases.
In Burkina Faso, three studies have investigated the prevalence of congenital malaria by LM: two independent cross-sectional studies reported prevalences of 1.4-2.8% in cord blood samples 20,21 , while a third study performed on hospitalized neonates during the first week of life reported a prevalence of 24.4% 9 . Routine diagnostic of clinical malaria in the country includes parasitological confirmation with rapid diagnostic test (RDT) and LM. Malaria parasite identification on Giemsa-stained blood smears is performed in district referral hospitals whereas the RDTs are the only diagnostic tool available for malaria at peripheral health centers. Although malaria RDTs have been implemented country-wide by the National Malaria Control Program (NMCP) since 2009, its performance for the detection of congenital malaria among neonates has not yet been evaluated.
In the present study, we determined the prevalence of congenital malaria, defined as cord-blood malaria infections as detected by quantitative PCR (qPCR) and we assessed the performance of a HRP2 RDT in plasma and LM to detect Plasmodium falciparum infections in cord blood samples. In addition, we used an Enzyme-linked Immunosorbent Assay (ELISA) kit to examine whether histidine-rich protein II antigen (PfHRP2) levels are good surrogate biomarkers of congenital malaria infections. Finally, we explored the association between cord-blood malaria infections as detected by qPCR and clinical outcomes.

Results
Characteristics of study participants. Characteristics of study participants are presented in Table 1. In total, 400 mother-child pairs were included in the study. The mean age of the pregnant women was 26.2 ± 6.1 years and the majority were multigravida (gravidity ≥ 3). Approximately one third of all pregnant women had a peripheral infection at the time of delivery and another third had placental infection as detected by qPCR. Few cases of pre-term deliveries were recorded (2%). The majority of deliveries (93.5%) occurred during the rainy season (July to October) and the post-rainy season (November to February) corresponding to the highest and moderate transmission intensity periods in the country, compared with the dry season (March to June). Among newborns, there was a slightly higher but non-significant proportion of females than males (P = 0.317). Their overall mean birth weight was 3013.8 g and 11.3% of newborns had a low birth weight (<2500 g).
Prevalence of congenital malaria by qPCR. The prevalence of congenital malaria, as detected by qPCR on cord-blood samples, was 4% (16/400) ( Table 2). These infections were significantly associated with both maternal peripheral infections (OR = 16, 95% CI: 3.6-71.4; P < 0.001) and placental-blood infections (OR = 29.4, 95% CI: 3.8-225.8; P < 0.001). The prevalence of congenital malaria increased to 10.9% (14/128) among newborns whose mothers had peripheral infections at delivery and to 10.2% (14/137) among those born from mothers with placental-blood infections. Additionally, we performed histological examination of placental tissue from those mothers with cord-blood parasitaemia (Table 3). From the 16 samples positive by qPCR all presented with placental infection: half of them had a past placental infection and the other half an active placental infection.

Characteristics Value
Mothers age-years, Mean ± SD 26.  Table 1. Characteristics of the mother-child pairs * . * The characteristics are described for the 400 mother-child pairs, unless indicated otherwise. SD, Standard Deviation; IQR, Interquartile Range; g, gram.
Parasite densities in the corresponding cord blood samples were <50 parasites/µL and >50 parasites/µL in past and active placental infections respectively. There was no significant difference in the prevalence of congenital malaria by gravidity (P = 0.980), although we observed a trend of decreasing prevalence in newborns from primigravid to multigravid women. The overall parasite densities in cord-blood infections cases were low with a geometric mean of 5.5 parasites/µL (95% CI: 1.4-22).
Diagnostic accuracy of RDT and LM for congenital malaria diagnosis. HRP2 was detected in cord-blood in 3 (0.75%) out of the 400 plasma samples tested by RDT, while LM failed to detect any infection in the study population (Table 3). Overall, RDT and LM accuracy to detect cord-blood infections was poor as  PfHRP2-levels in cord blood: antigen based testing and association with parasite densities. ELISA test for PfHRP2 antigen was positive in 6 (1.5%) out of the 400 tested cord blood samples (Table 3), although only 2/6 presented a confirmed qPCR cord-blood infection (Table 4). Among the 6 ELISA-positive samples, 3 were also positive by RDT performed in stored cord plasma samples (Table 3). RDT and ELISA were both positive when the antigen index determined by the ELISA test was >13 (Table 3). Indeed, samples that were found positive by RDT had a significantly higher antigen index than samples only found positive by qPCR and/ or ELISA (14.5 versus 0.5, P = 0.007).
In order to assess the appropriateness of using PfHRP2 as a biomarker for detection of congenital P. falciparum malaria infection, we investigated whether neonatal PfHRP2 levels in plasma were associated with the parasite densities in cord blood. The same association was tested for maternal blood parasite densities. The results suggested that the optical density (OD) of the PfHRP2-ELISA test could be associated with parasite densities from both cord and maternal blood, although OD values were much strongly associated with parasite densities in the mother (Kruskal Wallis coefficients = 52.5 and 58.7 for peripheral and placental blood respectively; P < 0.001) than those in cord blood (Kruskal Wallis coefficient = 10.4; P = 0.034). Indeed, as shown in Fig. 1, median PfHRP2 ODs followed a consistent trend towards increasing parasite densities in the placenta and maternal peripheral blood, while cord antigen levels showed a markedly different profile when compared to parasite density levels in cord blood. The strong correlation between cord antigen detection and the maternal parasite burden was further confirmed by comparing maternal parasite densities among ELISA-positive and qPCR-positive cases. As shown in Table 5, parasite densities, both in the placenta and in the peripheral blood, were significantly higher in mothers with a positive cord blood sample ELISA test (P = 0.001) than those with congenital malaria infection detected by qPCR (P < 0.05).

Congenital malaria by qPCR and clinical outcomes.
We investigated whether congenital malaria infections as detected by qPCR in cord-blood samples were associated with low birth weight (LBW), occurrence of fever and risk of malaria during the first two months of life. The overall prevalence of LBW in the study population was 11.3% (45/400 newborns). Although 18.7% (3/16) of the newborns with cord-blood infections were born with LBW, the association was non-significant (OR = 1.9, 95% CI: 0.5-6.9; P = 0.264). One neonate with congenital malaria by qPCR, developed clinical malaria at day 36 of the follow-up. Msp1 and msp2 genotyping of the paired samples, i.e. at birth and day 36, confirmed a new infection at day 36 indicating that the cord-blood infection at birth was cleared spontaneously without developing into a clinical case. In total, 10.5% (42/400) of children experienced at least one fever episode during the follow-up period and only two of them had cord-blood malaria infection. Finally, there was no evidence of association between fever episodes during the first two months of life and cord-blood infections (OR = 0.6, 95% CI = 0.3-5.4; P = 0.530).

Discussion
In this study we found a 4% prevalence of congenital malaria as detected by qPCR in cord-blood samples from mothers delivering in the Nanoro health district of Burkina Faso. Previous studies from Burkina Faso using LM to detect congenital malaria infections have reported lower prevalence estimates of 2.8% in Ouagadougou 21 and 1.4% in Boussé & Koupéla 20 , while a 24.4% prevalence was reported in a 10-year retrospective study, including only hospitalized sick neonates (<7 days) 9 . Several studies have discussed the impact of seasonality, diagnostic method and study design on the estimated prevalence of congenital malaria 3 . Although our study recruitment period is comparable to previous reports in the country 20  Pfvar genes (≈60 copies/genome) 22 , is 10 times more sensitive than the standard 18S rRNA-based qPCR used in other studies 23,24 and could explain at least partially variability observed between studies. On the other hand, the current study is ancillary to a larger trial investigating the effectiveness of strategies for malaria prevention in pregnancy 25 . Uptake of pregnancy preventive and control measures such as intermittent preventive treatment of malaria in pregnancy with sulfadoxine-pyrimethamine (IPTp-SP), insecticide treated net, malaria diagnosis and treatment is expected to be higher in the study population than in the general population due to the trial effect. These preventive measures, which are known to be effective in preventing maternal and placental malaria infections [26][27][28][29][30] , may have reduced the risk of infection and transplacental transmission of malaria parasites potentially leading to underestimation of congenital malaria prevalence. Indeed, cord-blood infections as detected by PCR were more often detected among mothers with maternal peripheral and placental infections at delivery (10.9% and 10.2% respectively) than in the general study population (4%). Although we cannot exclude that a number of these cord blood infections are due to admixture with maternal or placental blood at delivery, evidences of a compromised placenta, which increases the risk of transplacental passage [31][32][33] , found in all detected cord-blood infections and discordant levels of malaria parasitemia between cord and placental/maternal blood (with higher levels in cord than placental/maternal blood) are in agreement with malaria infections acquired antenatally 24 .
Therefore, due to the increased risk of congenital malaria found among newborns from infected women at delivery, this high risk group could be targeted for congenital malaria screening to allow for prompt and adequate neonatal clinical care. In this scenario, malaria diagnosis using umbilical cord-blood sample would be the best option to avoid invasive blood sample collection of the newborn. However, detection of congenital malaria infections in cord blood samples using field implemented diagnostic tools such RDT and LM remains a challenge.
LM is the standard method for malaria diagnosis in Burkina Faso and is available at the health district referral hospitals. In this study, LM did not detect any umbilical cord blood infections, contrasting with previous reports from Burkina Faso 20, 21 . The observed differences can be explained by the lower cord blood parasite densities    20,21 ). Lower parasite densities could also be explained by a higher uptake of pregnancy preventive measures in the present study. Thus, LM does not appear as an optimal tool to screen for congenital malaria infections in cord blood samples. Indeed, limited sensitivity of LM to detect congenital malaria infections compared to molecular methods has also been observed in studies conducted in other African countries 7, 23, 34 . The SD-Bioline PfHRP2 is the RDT recommended by the NMCP in Burkina Faso for malaria diagnosis in peripheral health centers in rural areas where there are no laboratory facilities available. Here, we assessed whether the use of PfHRP2-RDTs could be extended to screen umbilical cord-blood samples for P. falciparum detection. SD-Bioline PfHRP2 RDT missed 87.5% of umbilical cord-blood infections detected by qPCR indicating that diagnostic performance to detect congenital malaria is suboptimal. Moreover, RDT test was only found to be positive when PfHRP2 levels were very high (RDT positives >13 vs qPCR/ELISA positives <2). Of note, we did not follow manufacturers' instructions in the use of the PfHRP2 RDT as we tested stored cord plasma samples instead of whole blood, which may have decreased test sensitivity. However, we found a good agreement between RDT and ELISA tests taking into account that RDT uses lower plasma volumes than ELISA. This is in agreement with previous reports 35 and indicates that results obtained here are reliable. In the other hand, although we cannot exclude that RDT-or ELISA-negative/PCR-positive samples are due to the presence of pfhrp2-deleted P. falciparum parasites 36 , parasite densities detected by qPCR in cord blood samples are low and often under the limit of detection of the used RDT. Nevertheless, future studies should investigate the prevalence of pfhrp2-deletion particularly in areas of high malaria transmission as in the study area.
Only two other studies have previously evaluated RDT performance to detect congenital malaria infections 37,38 .
In Nigeria, pLDH-Optimal ® RDT did not detect any congenital malaria infection while prevalence by LM was 10.9% 37 and, in Burundi two newborns were found positive by SD-Bioline PfHRP2 but none by CareStart pan-pLDH 38 . When samples from these two newborns were subsequently tested by qPCR, both resulted negative and were resolved as false RDT-positives, even though WHO RDT performance evaluation reported no false positive SD Bioline Pf-antigen results after testing 140 true-negative samples 39 .Since PfHRP2-based tests sensitivity depends on antigen concentration and this seems to be determined by the parasite burden 40, 41 , we expected positive RDT cases among samples with high parasite density in umbilical cord blood. Surprisingly, congenital malaria infections presenting higher parasitemia (i.e. >100 parasites/µL; n = 3) were all negative by RDT, while RDT-positive cord-blood samples presented 79.8, 5.3 and 0 parasites/µL. Of note, the RDT-positive/ qPCR-negative cord sample corresponded to a mother with very high parasite burden at the time of delivery (14,342 parasites/µL and 1,489 parasites/µL in their placental and peripheral blood, respectively). This observation is in line with the findings reported in the study from Burundi 38 , where the two false-positive RDT results also corresponded to newborns from mothers with very high peripheral parasitemia (i.e. 99,520 and 454,061 parasites/µL). Indeed, a significant correlation between cord PfHRP2 levels and maternal parasite burden was further confirmed by our results. On the one hand, median placental and peripheral densities were found to be significantly higher among mother-child pairs with ELISA-positive result (P = 0.001) but not among those with a cord qPCR-positive result. In addition, cord PfHRP2 levels correlated strongly with maternal parasite densities than cord blood parasite densities. Unfortunately, we had no data available to investigate the direct correlation between cord blood and maternal PfHRP2 levels at delivery. All these evidences suggest that PfHRP2 in cord blood may be the result of the transplacental passage of maternal antigens rather than a reflection of concurrent malaria infection in the newborn. Hence, detection of congenital malaria infection based on the presence of PfHRP2 antigen might not be a reliable approach. Transplacental passage of malaria antigens was previously reported in a study conducted in The Gambia that found GLURP antigen in 28.6% of tested cord sera samples 42 . Likewise, transplacental transfer of circulating antigens has been also documented in other parasitic diseases such as lymphatic filariasis 43,44 . However, in the present study high maternal parasite densities do not always correlated with high PfHRP2 levels and positive RDT and/or ELISA results suggesting that transplacental passage of parasite antigens can be influenced by different conditions during pregnancy. In fact, factors that contribute to mother-to-child transmission of malaria parasites (or antigens) have not yet been well elucidated, although pre-existing level of malaria immunity, history of maternal infections and malaria-related placental changes and damage seems to play an important role [31][32][33][45][46][47][48] . In summary, our results indicate that PfHRP2 levels in cord blood samples are not a good surrogate biomarker of P. falciparum infections. Hence, even if high-sensitive PfHRP2-based RDTs are available on the market in the near future 49 , these would not render a useful tool for parasite presence detection in cord blood. Further investigations should include quantification of P. falciparum antigens levels such as (but not limited to) PfHRP2 in maternal peripheral, placental and umbilical cord blood samples.
Although there was a trend towards lower birth weight among newborns with congenital malaria, we did not find a significant association between LBW and cord-blood infections as detected by qPCR. The lack of association could be related to the low number of LBW newborns observed in our study population. We did not observe any case of clinical congenital malaria (presence of malaria parasites in symptomatic newborn during the first week of life) neither clinical neonatal malaria (parasites and symptoms from 1 to 4 weeks of life). One newborn with congenital malaria developed a clinical malaria infection 36 days after birth. However genotyping of paired samples concluded that the clinical episode at day 36 was due to a new infection.
Overall, the lack of a significant clinical impact of congenital malaria as detected by PCR on infant's health during the first 2 months of life in our study, may be due to the low number of congenital malaria infections. In the other hand, these results are concordant with other studies in high malaria transmission areas of sub-Saharan Africa, where symptomatic neonatal infections are rare suggesting that congenital malaria is infrequently associated with clinical disease 6,8,18 . The importance of maternal derived antibodies in controlling infections early in infancy could be one of the main explanations for the clearance of infections and the lack of association with symptoms and adverse outcomes after birth 50 . In addition, as in all studies based on high-sensitive detection of parasite DNA or antigen, we are indirectly detecting parasite material rather than alive parasites. Therefore, we cannot exclude that some of the positive results correspond to non-viable parasites 51 .
Prenatal exposure to blood-stage malaria parasites/antigens may have profound long-term effects during infancy and childhood by priming the immune responses of the fetus and/or by inducing immune tolerance 24,52 . However, the role of congenital malaria in the modulation of that immune response is unknown. Hence, a long-term effect of congenital malaria on newborns health living in high malaria endemic settings cannot be excluded and deserves further investigations. In the meantime, prenatal exposure to malaria parasites/antigens should be prevented through effective strategies for malaria control in pregnancy.
In conclusion, congenital malaria infection is not uncommon in Nanoro health district and the estimate of its prevalence using a high sensitive qPCR could reach 10% among newborns from mothers presenting peripheral and/or placental infections at delivery. The overall poor performance of SD-Bioline PfHRP2 RDT compared to qPCR suggests a limited utility of RDTs for congenital malaria screening in cord-blood samples in Burkina Faso. In addition, because PfHRP2 detection in cord blood could be influenced by high levels of parasite antigens in maternal blood through a transplacental passage, PfHRP2 might not be used as a surrogate of congenital malaria infection. There was no evidence of a significant clinical impact of congenital malaria infections on infant's health from birth to 59 days of life. Case-control studies including long-term follow up may provide additional understanding on the relevance of neonatal malaria infections.

Methods
Study site. This study was conducted in 10 peripheral health centers of Nanoro Health District (NHD) in Burkina Faso. The NHD comprises approximately 153,000 inhabitants and is located 85 km west of Ouagadougou, the country's capital. Malaria transmission in the region is seasonal and hyperendemic with the highest transmission period overlapping with the rainy season from July to October. The mean prevalence of malaria in the country is estimated to 62.2% with a mean entomological inoculation rate of 118.1 per person per year 53 .

Study participants.
This study is ancillary to a larger multicentric trial assessing the effectiveness of malaria in pregnancy preventive strategies in Burkina Faso, Benin and The Gambia. Details of the main trial protocol have been described elsewhere 25 . For the present study, 400 mother-child pairs were enrolled at the moment of delivery (from July 2014 to April 2015) after inform consent from the mothers was obtained and newborns were followed-up by passive case detection for 59 days to identify occurrence of fever or clinical malaria cases. Sample size was estimated assuming a minimum congenital malaria prevalence of 1.4% as reported in a previous study in Burkina Faso 20  Sample collection. At delivery, 200 µL of peripheral blood was obtained from the mother by finger-prick and placental blood was collected by pipette aspiration after incision of the maternal side of the placenta. A placental tissue section was collected from the maternal side and stored into 10% neutral buffer formalin. Cord blood was collected in heparin containing tubes by venipuncture of the umbilical vein and used to prepare thick blood smears for LM examination. Blood drops of all samples were spotted onto filter paper for posterior P. falciparum diagnosis by qPCR. One plasma aliquot was prepared from cord blood for ELISA and RDT test performance and stored at −80 °C. Newborns visiting the health center with presence or history of fever during the previous 24 hours were screened for malaria infection with RDT and, if positive, treated with artesunate-amodiaquine or artemether-lumefantrine according to national guidelines. In addition, a capillary blood sample was collected from finger-prick for qPCR analysis and LM examination.
Malaria Microscopy. Thick blood films stained with Giemsa (10%) were used for malaria detection by LM according to standard procedures 54 . Parasite density was expressed as the number of asexual parasites per μL of blood based on an assumed 8000 white blood cells per μL of blood. A slide was considered negative if no parasites were seen after examining 100 fields. Each slide was read by two independent experienced microscopists blinded to each other's results and in case of discrepancy, the slide was read by a third experienced microscopists. After the completion of the reads an internal quality control was performed by a fourth experienced reader for 10% of slides. Slides of the quality control included all positive samples detected by qPCR. Histological examination of placental tissue collected at delivery was performed as described elsewhere 33 . Rapid Diagnostic Test for malaria. RDT SD-Bioline malaria antigen P.f ® test (Standard Diagnostics, Inc, Korea) detecting PfHRP2 was used. Umbilical cord plasma was thawed at room temperature, centrifuged at 500 g for 10 min and five microliters were transferred into the RDT sample well, to which four drops of assay diluent were added. The RDT result was read in 15-30 min.
Enzyme-linked ImmunoSorbent Assay (ELISA) for PfHRP2. Detection of PfHRP2 antigen was performed with Malaria Ag CELISA TM kit (Cellabs, Brookvale, New South Wales, Australia) following manufacturer's recommendations. Briefly, 100 µL of umbilical cord plasma were tested and optical density (OD) read at 450 nm with Multiskan TM FC Microplate Photometer (Thermo Scientific). All samples and controls were tested in duplicate. Positive and negative controls were provided by the manufacturer and OD values were normalized using a reference positive control in all plates. Samples with a mean OD value above the cut-off level (OD of negative control +0.2 unit) were considered positive for PfHRP2 antigen as recommended by the manufacturer.
Scientific RepoRts | 7: 2080 | DOI:10.1038/s41598-017-02173-6 The antigen index values were determined by dividing the mean OD value of each sample by the cut-off value as previously described 55 . varATS real-time PCR (qPCR) for P. falciparum detection and quantification. For the molecular diagnosis by qPCR, filter papers with dried blood samples from maternal peripheral, placental and cord blood were punched and three circles of 5 mm in diameter was used for DNA extraction with QIAamp 96 DNA blood kit (Qiagen, Germany). Extracted DNA was eluted in 150μL of water. Five microliters of DNA were used for qPCR analysis targeting P. falciparum var gene acidic terminal sequence (varATS, ≈59 copies per genome) as previously described 22 . The limit of detection in our laboratory was 0.1 parasite/μL using DNA extracted from blood spot on filter paper. Parasite densities were obtained by extrapolating cycle thresholds (Ct) from a standard curve prepared with titrated samples containing known numbers of infected erythrocytes diluted in whole blood (100,000 to 0.1 parasites/μL). Samples with Ct value ≤39.7 were considered positive.
Parasites genotyping. Nested PCR targeting P. falciparum msp1 and msp2 genes was used to genotype parasite clones in a paired-sample corresponding to cord blood and peripheral blood of a newborn that experienced a malaria infection during the study follow-up. Family specific primers for msp1 (K1, MAD20 and R033) and msp2 (FC27 and IC(3D7)) were used according to the genotyping procedures recommended by WHO 56 . Data analysis and definition of terms. Data were double entered into the study databases (OpenClinica, community version or Excel, Microsoft, USA) and analyzed using Epi Info version 3.5.4 and STATA 12.0 (StataCorp, USA). Normally distributed data was described by mean ± SD and non-normally distributed data was described by the median with interquartile range (IQR). Congenital malaria prevalences were calculated with 95% Confidence Interval (CI). Parasite densities and ELISA optical densities (ODs) were not normally distributed even after logarithm transformation. Thus, the difference in median values of parasite density and ODs between groups were analyzed using the non-parametric Mann-Whitney and Kruskal Wallis tests. Sensitivity and specificity were calculated with 95% CI to compare RDT and LM against qPCR. P values < 0.05 were considered statistically significant. The following definitions were applied: (a) Congenital malaria infection: cord-blood malaria infections as detected by quantitative PCR; (b) Maternal peripheral and placental infections: presence of parasite DNA detected by qPCR in the peripheral and placental blood at delivery; (c) Low birth weight: <2,500 g; (d) Fever: axillary temperature ≥37.5 °C; (e) Clinical malaria episode: presence of fever at the moment of screening or during the previous 24 h and positive test by RDT, LM or qPCR.