Figure 3 | Scientific Reports

Figure 3

From: Adaptation to dietary conditions by trehalose metabolism in Drosophila

Figure 3

Treh II-8 and Treh X-2 mutants are indistinguishable from Treh cs1 mutants. (A) The majority of Treh II-8 and Treh X-2 mutants are lethal at the pupal stage. Percentages of homozygous mutant pupae were determined by the ratio to heterozygous mutants in each vial. No statistical significances were detected by one-way ANOVA with Tukey’s post hoc test (p < 0.01). n = 6. (B) Treh X-2 and Treh cs1 mutants at late-third larval and pupal stages are shown. (C) One copy of the genomic rescue construct of Treh-HA rescues the pupal lethality of Treh cs1 homozygous mutants. (D) Survival of male flies with one copy or two copies of the genomic rescue constructs of Treh-HA in a Treh cs1 homozygous mutant background. Values shown are means and SEM. n = 4. (E) Treh II-8 and Treh X-2 mutants exhibit the identical phenotype to Treh cs1, as revealed by the increase in trehalose levels and decrease in glucose levels in late third instar larvae. Each value was normalized by protein levels and further normalized according to the levels in the control larvae. (F) Trehalase activity was measured in larval homogenates. Relative levels of trehalose are shown for each mutant. Values are means and SD. n = 4 (E,F). *p < 0.01; one-way ANOVA with Dunnett’s post hoc test.

Back to article page