18 is a hypomorphic allele of Treh. (A) Backcrossed Treh
18 mutants exhibit pupal lethality with few escapers. The percentage of adult flies was determined by the ratio to flies with a balancer chromosome in each vial. Values shown are means and SEM. n = 6. (B) Treh
18 mutants increase trehalose levels and decrease glucose levels in late third instar larvae. Each value was normalized by protein levels and further normalized according to the level in the control larvae. (C) Treh transcript levels were analyzed by qRT-PCR at the mid third instar stage. Values are means and SD. n = 3 (B,C). *p < 0.01; (B) one-way ANOVA with Dunnett’s post hoc test, (C) two-tailed Student’s t-test.