Efficient Antibacterial Membrane based on Two-Dimensional Ti3C2Tx (MXene) Nanosheets


Advanced membranes that enable ultrafast water flux while demonstrating anti-biofouling characteristics can facilitate sustainable water/wastewater treatment processes. MXenes, two-dimensional (2D) metal carbides and nitrides, have attracted attention for applications in water/wastewater treatment. In this work, we reported the antibacterial properties of micrometer-thick titanium carbide (Ti3C2Tx) MXene membranes prepared by filtration on a polyvinylidene fluoride (PVDF) support. The bactericidal properties of Ti3C2Tx modified membranes were tested against Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) by bacterial growth on the membrane surface and its exposure to bacterial suspensions. The antibacterial rate of fresh Ti3C2Tx MXene membranes reaches more than 73% against B. subtilis and 67% against E. coli as compared with that of control PVDF, while aged Ti3C2Tx membrane showed over 99% growth inhibition of both bacteria under same conditions. Flow cytometry showed about 70% population of dead and compromised cells after 24 h of exposure of both bacterial strains. The damage of the cell surfaces was also revealed by scanning electron microscopy (SEM) and atomic force microscopy (AFM) analysis, respectively. The demonstrated antibacterial activity of MXene coated membranes against common waterborne bacteria, promotes their potential application as anti-biofouling membrane in water and wastewater treatment processes.


Bactericidal nanomaterials are widely explored effectively in public health applications including medical devices, water treatment, food packaging, and in the textile industries1,2,3. Among a wide spectrum of nanomaterials with proven bactericidal efficacy, antibacterial properties of 2D nanosheets, including MXenes, graphenes and MoS2 have been explored to meet these challenges4,5,6. Several groups have also shown that decorating nanoparticles on the surface of 2D architectures including graphene oxide (GO) increases its antimicrobial effect7,8,9. The antimicrobial activities of MoS2 and graphene-based materials, including graphite, graphite oxide, graphene oxide (GO), and reduced GO (rGO), against Gram-negative and Gram-positive bacteria have been found to be the synergy of both “chemical” and “physical” factors6, 10,11,12,13,14,15,16. Most of the above studies have attributed the antibacterial activity of GO and rGO to cellular membrane stress induced by sharp edges of graphene nanosheets, which may result in physical damage of cell membranes, leading to a loss of bacterial membrane integrity2, 17,18,19,20.

MXenes are a family of two-dimensional (2D) transition metal carbides and nitrides with a common formula of Mn+1XnTx, where M is an early transition metal, X is C and/or N, n = 1, 2 or 3, and Tx represents surface functional groups, such as F, OH, or O21. MXenes combine a hydrophilic surface, metallic conductivity, and a high capacity for ion adsorption, which was proven by the reversible intercalation of cations (e.g., Li+, Na+, K+, Mg2+, etc.). These properties render MXene a promising candidate for environmental remediation applications21. Ti3C2Tx MXene has been widely explored in several applications including heavy metal adsorption22,23,24 and photodegradation of dyes25. A biocompatible composite based on soybean phospholipid modified Ti3C2 nanosheets was recently used for cancer therapy26. Highly flexible and ionically conductive MXene membranes with layered nanosheets showed selective sieving of high valence ions27. Recently, Ti3C2Tx membranes with controlled thicknesses, flexibility, and high mechanical strength with unique separation properties were revealed28. According to Ding et al., a MXene membrane with stacked thin layers achieved a high rejection rate (90%) for large size molecules (>2.5 nm) while maintaining over 1000 L m2 h−1 bar−1water permeance29. A major success indicator for any water treatment membrane is the resistance to biofouling caused by living organisms. Colloidal Ti3C2Tx showed high antibacterial properties against E. coli and B. subtilis, as confirmed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) coupled with lactate dehydrogenase (LDH) release assay indicated the damage to the bacterium cellular membrane5. Nevertheless, biofouling is a major limitation for separation membrane development30. Bacteria and other microorganisms adhere to the membrane surface and form a viscous gel-like biofilm causing a severe decline in flux30.

Here, in an effort to advocate the potential of 2D metal carbides for use in water purification membranes, we investigate for the first time the antibacterial activity of Ti3C2Tx modified membranes by taking into consideration the bactericidal activity of the colloidal Ti3C2Tx shown in our earlier study5. The antimicrobial activities of various membranes were investigated against Gram-negative and Gram-positive bacteria by filtering certain concentrations of bacterial suspensions through the PVDF and PVDF-supported Ti3C2Tx membranes. The latter were fabricated by vacuum-assisted filtration (VAF) and their ability to inhibit E. coli and B. subtilis bacterial growth was studied. The interactions between MXene 2D nanosheets and bacteria have been investigated by SEM, AFM, and flow cytometery.

Results and Discussions

Physical and chemical characterization of Ti3C2Tx based membranes

In order to obtain uniform film coating on PVDF as shown in Fig. 1A, a dilute colloidal Ti3C2Tx solution (~0.01 mg/mL) was used31. The dilute solutions contained primarily single-layer Ti3C2Tx sheets with thickness on the order of 1 nm and lateral sizes on the order of hundreds of nanometers to several microns32. The high aspect ratio of the nanosheets ensures uniform and narrow 2D nanochannels and mitigates the presence of meso- and macro-pores across the membrane (Fig. 1B). TEM image in Fig. 1C is showing a single flake of delaminated Ti3C2Tx, with lateral sizes up to a few hundred nanometers. Considering the relatively high pressure exerted on Ti3C2Tx membranes during the experimental procedure, commercial polyvinylidene fluoride (PVDF) supports (450 nm pores) were used31. The hydrophilicity of the membrane was evaluated by measuring the water contact angle of pristine PVDF membranes and after coating with Ti3C2Tx film. PVDF was hydrophobic with a contact angle of 81°. On the other hand, Ti3C2Tx coated membrane functionalization significantly increased the hydrophilicity of the membrane, decreasing the water contact angle to 37°. AFM was used to compare the surface roughness patterns of pristine PVDF and Ti3C2Tx/PVDF membranes (Fig. 1D,E). Upon Ti3C2Tx coating, the membrane surface became rougher with RMS value increasing from 295 to 343, due to MXene wrinkles and edges that are also seen in the SEM image (Fig. 1B). Energy-dispersive X-ray spectroscopy (EDS) analysis confirmed the presence of Ti3C2Tx in the elemental composition onto the PVDF surface indicated by signature Ti peak at 4.5 KeV (Fig. 1F). X-ray diffraction (XRD) patterns of air-dried Ti3C2Tx powder and Ti3C2Tx/PVDF membrane are shown in Fig. 1G. The large (00 l) peaks at 5.8 degree from basal planes of MXene are characteristic of delaminated Ti3C2Tx with layers of water between the sheets. The peak of Ti3C2Tx/PVDF is broader than pristine delaminated Ti3C2Tx and has been shifted to a higher 2θ angle, from 5.8 to 6.6 degree, corresponding to a decrease of the c-lattice parameter for Ti3C2Tx on PVDF as compared with pristine Ti3C2Tx 33, 34, indicating a decrease of interlayer spacing in the MXene film on PVDF28. Characteristic peaks of Ti3C2Tx from 18 to 40° are still observed, which suggests a good periodicity between the stacked MXene layers. Other prominent peaks from the Ti3C2Tx/PVDF membrane are characteristic of the supporting PVDF35.

Figure 1

(A) Schematic of the Ti3C2Tx membrane fabrication on a PVDF support; (B) Cross-sectional SEM image of a Ti3C2Tx film; (C) TEM image of a pristine Ti3C2Tx flake; (D and E) 3D AFM images of a PVDF membrane and a Ti3C2Tx/PVDF film, respectively; (F) EDX spectrum of Ti3C2Tx/PVDF; (G) XRD patterns of Ti3C2Tx and a Ti3C2Tx/PVDF membrane.

Antibacterial activity of Ti3C2Tx membrane

Figure 2 depicts the antibacterial activities of Ti3C2Tx coated membranes against E. coli and B. subtilis as compared with the PVDF control after being used for filtering 104 CFU.mL−1 bacterial solution. A significant decrease in count for viable colonies of both bacteria on membrane surface after 24 h incubation with Ti3C2Tx modified membranes was observed (Fig. 2A). Therefore, Ti3C2Tx films were able to inhibit bacteria growth and reduce viability of bacterial cells as compared with pristine PVDF membranes. The growth inhibition of Ti3C2Tx membranes reaches about 73% against B. subtilis and 67% against E. coli as compared to that of control PVDF (Fig. 2B). This shows the antibacterial activity of Ti3C2Tx coated membranes against both Gram-positive and Gram-negative bacterial strains. Differences in antibacterial activity against E. coli and B. subtilis can be related to their different cell wall structure. For instance, Gram-negative E. coli have a thin layer of peptidoglycan (2–3 nm) between the inner and outer cell membranes, whereas Gram-positive B. subtilis have a thicker peptidoglycan layer (20–80 nm), resulting in a higher resistance towards Ti3C2Tx film. Additionally, there was no growth of bacteria in the infiltrate through both PVDF and Ti3C2Tx/PVDF membranes assuming 100% retention. Another set of experiments was performed to investigate the effect of Ti3C2Tx film thickness on its antibacterial activity. As expected, increasing the Ti3C2Tx thickness from 0.6 to 1.8 µm showed no significant effect on antibacterial activity (Supplemental Material, Figure. S1), indicating that the bacteria are exposed to the surface layer of the membrane. 1.2 µm Ti3C2Tx coating has been used in this study as an optimal membrane thickness. In our earlier study, 1.2 µm Ti3C2Tx coating demonstrated ultrafast water flux of 37.4 L · Bar−1 · h−1 · m−2 with charge selective ions sieving depending on both the hydration radius and charge of the ions28. Antibacterial activity of Ti3C2Tx nanosheets in suspension has been elaborated in our earlier study5, however, this is the first report to evaluate the antibacterial activity of Ti3C2Tx MXene coated membranes.

Figure 2

Antibacterial activity of Ti3C2Tx MXene. (A) Photographs of E. coli and B. subtilis growth on unmodified PVDF (control), and fresh and aged Ti3C2Tx MXene coated PVDF membranes incubated at 35 °C for 24 h. (B) Cell viability measurements of E. coli and B. subtilis grown on fresh and aged Ti3C2Tx MXene coated PVDF membranes for 24 h. Survival rates were obtained by the colony forming count method. Error bars represent the standard deviation of triplicate experiments.

Effect of environmental condition on membrane efficiency is also an important factor. So it was initially assumed that oxidation of the membrane surface and formation of titanium oxide may decrease the antibacterial activity of the membrane surface. Controlled oxidation of Ti3C2Tx in air results in the formation of anatase TiO2 nanocrystals embedded in amorphous TiO2-C36,37,38,39. Ghassemi et al., reported the formation of thin anatase nanoparticles, and the sheets of nanocrystalline rutile by oxidation of top and bottom Ti layers under the flash and slow oxidation regimes, respectively40. XPS studies revealed that aged MXene films were surrounded by a thin layer of oxides together with graphitic carbon, which helped to maintain conductive contact between MXene particles41. The amount of oxide is dependent on the exposure time to ambient air32. To investigate the impact of membrane stability on the bactericidal efficiency, fresh membranes were stored at ambient air and room temperature for over 30 days. After which, the antibacterial activity of aged Ti3C2Tx membranes was investigated in the same manner described above. As shown in Fig. 2, the growth of bacterial colonies on the aged Ti3C2Tx membranes was hindered as compared to fresh membranes: >99% bacterial growth inhibition of both E. coli and B. subtilis was observed for aged membranes as compared to fresh membranes which showed 73% growth inhibition for B. subtilis and 67% for E. coli. This is a very important finding showing that aging of the membrane is advantageous to enhance the overall antibacterial properties. This could be attributed to the presence of TiO2 nanocrystals on the Ti3C2Tx membrane surface. Similarly, titanium substrates coated with nanostructured TiO2 showed a significant reduction in E. coli accumulation over large areas42.

To further investigate the interaction of the membrane surface with bacteria, the surface morphologies of uncoated PVDF and Ti3C2Tx/PVDF membranes after 24 h of incubation were examined by SEM (Fig. 3). The bacterial cells on Ti3C2Tx modified membranes showed evident differences compared to the control PVDF. Cell density of E. coli and B. subtilis grown on Ti3C2Tx modified membranes was significantly lower as compared to that of control PVDF membranes. It was also found that the cells on the pristine PVDF surface were smooth, intact and viable without any membrane disruption. However, few bacterial cells survived on Ti3C2Tx surfaces, as indicated by prevalent cell membrane damage. The bacteria on Ti3C2Tx film have a rough surface (Fig. 3B), indicating their damage, and some bacteria were even totally burst. Previously, we provided evidence that bacterial cells were ruptured by MXene nanosheets, which could cause the leakage of internal cell contents5. It was found that the number of bacterial cells in contact with Ti3C2Tx/PVDF decreased as compared with that of PVDF control. As expected, the rough Ti3C2Tx film caused rupture of bacterial membrane and the intracellular densities of E. coli and B. subtilis decreased as seen from the higher magnification panels, revealing that they lost some intracellular substance5. This indicates that the MXene membrane can inhibit the bacterial growth and efficiently hinder the biofilm formation, which is important for water purification membranes.

Figure 3

SEM images of the E. coli (top panels) and B. subtilis (bottom panels) colonies grown on (A) PVDF and (B) Ti3C2Tx modified membranes, at low and high magnifications. Control bacterial cells were viable with no observed membrane damage or cell death, and the higher magnification images show that the bacteria were protected by intact cytoplasmic membrane.

As further evidence, AFM scans were collected for the bacteria grown on the Ti3C2Tx/PVDF as well as pristine PVDF membranes. Representative micrographs are shown in Fig. 4. For the control PVDF membranes, intact bacteria can be seen on the surface for both E. coli and B. subtilis. However, bacteria on Ti3C2Tx film were smaller and showed signs of structural damage. 0.5 μm images were cropped and split from bacterial surface scans and re-flattened, to allow evaluation of the bacterial surfaces, independent of the underlying membrane support. For E. coli on the control surface, the structure of the bacterial wall resembles the porous ‘filigree’ structure of peptidoglycan previously reported by other researchers43. For corresponding images obtained from E. coli on Ti3C2Tx, this structure is not apparent, replaced by a less ordered globular arrangement of components, suggesting damage has occurred to the outer structure of the cell. For B. subtilis on the control surface a different (filamentous) surface structure is seen, compared to that observed for E. coli. It is similar to the ‘cabling’ structures formed from peptidoglycan previously observed by other researchers for B. subtilis 44. However, it must be noted that the cabling arrangements were previously observed on the inner surface of the cells alone. For images obtained from the bacterial surface on the Ti3C2Tx film, the surface structure appears to be less ordered.

Figure 4

AFM images of E. coli and B. subtilis colonies grown on PVDF and Ti3C2Tx modified PVDF membranes. The black square areas indicate where a 0.5 × 0.5 μm zoom scan has been performed (right hand of each panel); showing fine detail of the bacterial capsule surfaces.

Roughness values on the surface of bacterial membranes for both E. coli and B. subtilis are reported in Table 1 (mean values of 9 measurements for each sample). In both cases the root mean squared height (Sq) values increased for the bacteria on Ti3C2Tx compared with PVDF surfaces, indicating the change in bacterial surface morphology. Student’s t-tests were carried out, assuming two-tailed sample distributions with unequal variances, with the change to the E. coli surfaces being found to be more significant (p < 0.001) than for the B. subtilis (p = 0.06). The low significance of the Sq difference for B. subtilis does not reflect the different surface structure seen in the height images, where the surfaces appear very different. It should be noted that Sq is essentially the standard deviation of the heights of image data points9. As a result it is possible to have surfaces with different topographies having similar values of Sq.

Table 1 Roughness values on the surface of bacterial membrane for both E. coli and B. subtilis.

The PeakForce operating mode of the AFM allows access to nano-mechanical data simultaneously to height imaging. The mean values of the sample deformation for the same areas as for the Sq data are presented in Table 1. Each value represents an average of 9 image-mean values. The deformation values are the calculated penetration depth of the probe into the sample surface at the peak load values for each tip-sample interaction event, with the peak force kept at a constant set-point for all measurements (90 nN). As such, a higher deformation value represents a less rigid surface with a lower elastic modulus. For both bacteria, the deformation was significantly greater for bacteria on Ti3C2Tx surfaces than for those on the PVDF control. A two-sample t-test assuming unequal variances showed a high degree of statistical significance for both bacteria, with p values of 0.015 and 0.0003 for the E. coli and B. subtilis samples, respectively. This “softening” of the bacteria is most likely a result of the damage to bacterial cells observed in the SEM and AFM imaging and reflects the decrease in the cells viability due to interaction with the Ti3C2Tx film.

Next, we evaluated the antimicrobial kinetics of Ti3C2Tx coated membranes. A series of batch shake experiments were performed with E. coli and B. subtilis suspensions exposed to Ti3C2Tx/PVDF and PVDF membranes. Figure 5 depicts the growth of bacteria after exposure to Ti3C2Tx coated membrane at different time intervals which is measured based on the colonies growth on agar nutrient media. There was a lower bactericidal activity within the first two hours of incubation in both E. coli and B. subtilis. However, an evident decrease was observed in bacterial colonies growth on Ti3C2Tx films with the progression of time. After 24 h of contact time, the bacterial cell viability for both E. coli decreased to 18 ± 2.23%, whereas, for B. subtilis it was approximately 14.45% as compared to that of control PVDF membrane. This shows the pronounced inactivation of both bacterial strains. According to these results, Gram-negative bacteria seemed to have a higher resistance against MXene membranes as compared to Gram-positive bacteria. These findings are in line with the previous studies for Ti3C2Tx MXene dispersions5.

Figure 5

Cell viability measurements of (A) E. coli and (B) B. subtilis exposed to MXene membranes at different time intervals during 24 h of contact time. Survival rates were obtained by the colony forming count method as compared to that of control PVDF membrane. Error bars represent the standard deviation. First order rate plot for the inactivation of (C) E. coli and (D) B. subtilis.

Based on the experimental data, the first-order Chick’s law45, which expresses the main principles of bacterial inactivation kinetics, was tested to determine the inactivation rate constants. Figure 5(C and D) depicts the bactericidal kinetics of MXene coated membranes as compared to that of PVDF control. The rate of inactivation of E. coli and B. subtilis was calculated using Chick’s law, with ln(N/N 0 ) plotted as a function of time; where N is the number of bacterial cells at a given time and N 0 is the initial bacterial cell count. The first order rate constant, k, was calculated per unit area of membrane and the inactivation rate for B. subtilis was 0.0723 h−1.cm−1 - somewhat higher compared to that of E. coli (0.0677 h−1.cm−1).

Cytometric measurement of cell membrane damage

A mechanistic investigation of the interaction of Ti3C2Tx nanosheets with both bacterial cells was obtained from the flow cytometric analysis. The measurements of fluorescence reveal the populations of live, compromised and dead cells, leading to better understanding of the working of the antimicrobial agent. The dot plots in Fig. 6 show populations of E. coli and B. subtilis cells stained with PI and SYBR green, measured by flow cytometery after 24 h exposure to Ti3C2Tx coated membranes. Figure 6 shows the particular fluorescence patterns of B. subtilis and E. coli, doubly stained with SYBR green and PI. The low red and strong green fluorescence intensity region (P1) depicted the proportion of live bacteria, and the weak green and heavy red fluorescence intensity region (P2) indicated the proportion of the dead cells. Bacterial cells exposed to Ti3C2Tx coated membranes for 24 h showed a shift of population from viable to dead and compromised cells. For E. coli, 92.0% of cells with control PVDF membrane fell in the region P1, whereas the P1 values were 32% for bacteria exposed to Ti3C2Tx film. In the case of B. subtilis, the control presented 90% in P1 region (live cells), 3% in P2 region and remaining of compromised bacteria. Bacterial cells exposed to Ti3C2Tx film showed about 45% population of dead cells after 24 h of exposure for both E. coli and B. subtilis, whereas, almost 30% of the total cell population of both bacterial strains seemed to have been compromised. The group of bacteria in contact with PVDF control exhibited a much smaller number of compromised cells. Results showed negligible amount of dead or lysed cells of E. coli and B. subtilis in the control samples. Bacterial permeability to propidium iodide (PI) indicated the alteration and occurrence of substantial damage to the cell membrane, which finally causes cell death46. Ti3C2Tx coated membrane caused irreparable damage to the bacterial cell membrane resulting in growth inhibition.

Figure 6

Cell viability measurement of E. coli and B. subtilis exposed to PVDF and Ti3C2Tx. Flow cytometry dot plots of E. coli (A,C) and B. subtilis (B,D) bacterial cells exposed to control PVDF (A and B) and MXene films (C,D) for 24 h.

Evidenced by flow cytometry, SEM and AFM analysis, a thin layer of Ti3C2Tx MXene sheets on the membrane surface seems to be very efficient in inhibiting bacterial growth. Surface oxidation of aged Ti3C2Tx coated membranes demonstrated higher antibacterial efficiency as compared with the fresh membrane. This is most likely attributed to formation of anatase TiO2 nanocrystals and highly defective 2D carbon structure47, which helps in bacterial inhibition, possibly by the direct physical contact of MXene and TiO2 sharp edges (see ref. 47) with the bacterial surface, thereby causing physical stress and disruption of cellular membranes. Also, oxidative stress cannot be ruled out in case of aged Ti3C2Tx membranes since TiO2 can stimulate the oxidative stress on bacterial surface due to possible radicals formation37. A number of studies have explored the antibacterial activity of membranes based on nanomaterials like carbon nanotubes (CNTs), graphene and their nanocomposites used for filtration and separation applications11, 48. Similarly, graphene oxide coatings can significantly improve the antibacterial properties of commercial membrane filters49. The primary antibacterial activity of the nanomaterial modified membranes in the studies discussed above have been explained by the direct contact of nanosheets and/or nanotubes with the bacterial surface, thus causing physical stress and membrane damage. The hydrophilicity of the Ti3C2Tx surface may facilitate inactivation of bacteria by direct contact interaction. The high density of defects on MXene could also enhance the antibacterial activity48, 50. Also, MXene surfaces are covered by functional groups, some of which can be quite reactive, especially in contact with basic environment (e.g., Ti-F)41. Ti3C2Tx MXene has also been shown to adsorb and degrade positively charged dye molecules25. It’s a strong reducing agent and a variety of chemical interactions with bacterial membranes may be possible, but they need further studies. Here we introduce the potential of MXene as a material for anti-biofouling membranes; however, a comprehensive understanding of MXene’s behavior under different operating environments is important for future applications in water/wastewater treatment. For instance, controlling the functional groups, lateral size, number of layers, and surface and edge properties of MXene nanosheets may allow controlling its antibacterial activity. Ti3C2Tx MXene is only one of about 20 MXenes reported to date and new structures are added to the list every couple of months21.


Hydrophilic Ti3C2Tx MXene coatings have excellent antibacterial activity against both, E. coli and B. subtilis. Surface oxidation of aged membrane showed a significant improvement of antibacterial activity as compared with the freshly prepared membranes. This has been attributed to the synergistic effect between Ti3C2Tx nanosheets and TiO2/C formed on the surface. As evidenced by flow cytometery, colony forming counts, SEM and AFM analysis, MXene inactivated the bacterial growth and caused cell death on the membrane surface. MXenes, as a new family of 2D materials, may open a door for developing efficient antibacterial membranes for water and wastewater treatment, as well as other applications.

Materials and Methods

Synthesis, delamination and dispersion of Ti3C2Tx MXene

A colloidal solution of single- and few-layer Ti3C2Tx particles was obtained by delaminating the multilayer Ti3C2Tx powders by ultrasonication after etching Ti3AlC2 MAX phase with LiF/HCl solution, as described previously, with minor modifications in the process32. Briefly, the obtained Ti3C2Tx powder was dispersed in deaerated water with a weight ratio of Ti3C2Tx:water of 1:250. The suspension was sonicated under flowing argon and then centrifuged for 1 h at 3000 rpm to obtain the supernatant containing Ti3C2Tx flakes. TEM, SEM, energy-dispersive X-ray spectroscopy (EDX), and XRD were used to confirm the morphology of the flakes.

Preparation of Ti3C2Tx membranes

The MXene membranes were prepared by the VAF. Typically, the delaminated Ti3C2Tx solution was diluted to 0.01 mg.mL−1 and filtered through a commercial PVDF membrane (Hydrophilic, 0.45 µm pore size, EMD Millipore Durapore, US) with a diameter of 47 mm. The membranes with different thickness were prepared by depositing 2, 4 and 6 mg of Ti3C2Tx on 47 mm diameter PVDF to obtain average thickness of 0.6, 1.2, and 1.8 µm, respectively. A glass microfiltration apparatus, with a fritted alumina supported base 40 mm diameter, was used for VAF. The filtered membranes were air-dried and used for further antibacterial experiments on the PVDF support.

Cell cultures

The antibacterial properties of Ti3C2Tx membranes were evaluated using E. coli and B. subtilis as the model gram-negative and Gram-positive bacteria, respectively. Glycerol stocks were used to inoculate defined overnight cultures in Luria-Bertani broth (LB) medium at 35 °C. Following that, 1 mL of cell suspensions were sub-cultured and harvested during the exponential growth phase. Cultures were centrifuged at 5000 rpm for 5 min and pellets obtained were washed three times with phosphate buffer saline (PBS) (pH = 7.2) to remove residual macromolecules and other growth medium constituents. The cell pellets collected by centrifugation were re-suspended in sterilized PBS and diluted to cell concentration of approximately 107 colony forming units (CFU).mL−1.

Evaluation of antibacterial activity

The antibacterial activity of Ti3C2Tx modified membranes was investigated against Gram-negative bacteria E. coli and Gram-positive bacteria B. subtilis by two methods: bacterial filtration through membranes and direct bacterial suspensions with MXene films. In first assay, both E. coli and B. subtilis cells were diluted to 104 CFU.mL−1 in PBS. Typically, 50 µL of bacterial suspensions were further diluted in PBS to make total 10 mL solution and after this suction-filtered through the PVDF and MXene modified membranes. Membranes with bacteria on the surface were air dried for 10 min and placed on LB agar plates and incubated overnight at 35 °C. Finally, the growths of the colonies on the membrane surface were observed. The filtrate was also collected for subsequent analysis of bacterial cell presence by spreading 100 µL on agar nutrient media. The filtration assembly and glassware used were autoclaved and the membrane samples were sterilized with UV irradiation for 30 min before the experiments. To avoid the contamination of E. coli and B. subtilis cells, separate filtration assemblies were used for each bacterial assay. To avoid rupture of the membranes, the filtration was operated under a low pressure of about 2 kPa. After incubation for 24 h at 35 °C, the number of colonies grown on membrane surface was counted to determine the antimicrobial efficiency of Ti3C2Tx membranes. To investigate the effect of Ti3C2Tx membrane aging and surface oxidation on its antibacterial activity, the Ti3C2Tx modified membranes were prepared and allowed to age by exposing to ambient atmosphere for over a month. The antibacterial characteristics of aged membranes were studied as described above.

In the second set of bactericidal assay, several samples of Ti3C2Tx coated membranes (1.5 cm2) were suspended in E. coli and B. subtilis (107 CFU.mL−1) in saline and incubated at 35 °C under constant shaking at 110 rpm for up to 24 h. A control assay with bacteria without any membrane was used as a negative control, and the second with the bacteria in saline in the presence of PVDF membrane was used as a positive control. To examine the effect of contact time on bactericidal activity, membranes were taken out at 2, 4, 8, 16 or 24 h of incubation and gently washed with saline solution to remove loosely bound bacteria. The membranes were then placed in 2 mL of PBS and sonicated for 2–3 min to detach the bacteria from the membrane surface. The viability of cells attached to the membrane surface was analyzed by spreading 100 μL of suspension on nutritive agar plates after overnight incubation at 35 °C. The experiments were carried out in triplicate and average values were reported. The antibacterial activity was calculated using the following equation:

$$R=\frac{({N}_{c}-{N}_{m})}{{N}_{c}}\times 100,$$

where, N c and N m correspond to the number of colonies incubated with the PVDF (control) and MXene modified membranes for a given duration of treatment, respectively.

Flow cytometric assay for bacterial viability analysis

Viability of bacterial cells and disruption of membrane integrity were evaluated using propidium iodide (PI), and SYBR green. PI enters only permeable cells, binds DNA, and fluoresces at 620 nm, when stimulated by a laser at 488 nm whereas SYBR green can stain the total bacteria. Briefly, several tubes containing 2 mL (1 × 107 CFU.mL−1) of the E. coli and B. subtilis cells and membranes (1.5 cm2) were incubated at 35 °C for 24 h. After incubation, the membranes were gently washed with PBS to remove loosely bound bacteria. Washed membranes were placed in 2 mL of PBS and sonicated for 2–3 min to detach the bacteria from the membrane surface. Viability of the cells attached to the membrane surface was analyzed by flow cytometery. SYBR green (10,000 × stock) (10 µL) was mixed with 30 µL PI (20 mM) into 1.0 mL of sterile dH2O and vortexed thoroughly. All the samples were stained with PI and SYBR green to achieve the concentration of 0.3 mM and 1x, respectively. Samples were analyzed by a flow cytometer (BD CSampler, Accuri,). They were illuminated with a 15 mW argon ion laser (488 nm), and the fluorescence was detected via 525 ± 10 nm (green) and 620 ± 10 nm (red) band pass filters. Signals were amplified with the logarithmic mode for side scattering, forward scattering, and fluorescence. In dot plots of fluorescence, different bacterial populations were gated according to the viability stages.

Cell morphology observation with SEM and AFM

SEM analysis was performed to observe the effect of Ti3C2Tx MXene on morphology and surface structure of the bacterial cells using FEI-Nova Nano SEM 650. SEM imaging of samples was accomplished using the following procedures. Following the experiments, cells on both PVDF and Ti3C2Tx coated membrane surfaces were fixed with 2.5% glutaraldehyde for 4 h at 4 °C, followed by washing with 0.1 M phosphate buffer (pH 7.4) and dehydration with a graded ethanol series (25, 50, 80, 100%). Samples were allowed to dry completely at room temperature and were then coated with gold by sputtering (5 nm).

AFM characterization was carried out using a Dimension Icon model AFM with NanoScope V Controller (Bruker AXS, USA) operating in PeakForce mode. All measurements were made in ambient conditions using NSG30 silicon tapping mode probes (NT-MDT, Russia). Samples, which had been fixed using the identical procedure for that of SEM samples but without gold sputter-coating step, were immobilised onto glass sides using double-sided tape, which was in turn fixed onto the sample stage using instrument vacuum. All height images were flattened using 2nd order levelling to remove background offset and sample tilt prior to further analysis.


  1. 1.

    Hajipour, M. J. et al. Antibacterial properties of nanoparticles. Trends Biotechnol 30, 499–511, doi:10.1016/j.tibtech.2012.06.004 (2012).

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Li, Q. et al. Antimicrobial nanomaterials for water disinfection and microbial control: Potential applications and implications. Water Res 42, 4591–4602, doi:10.1016/j.watres.2008.08.015 (2008).

    ADS  CAS  Article  PubMed  Google Scholar 

  3. 3.

    Rasool, K. & Lee, D. S. Effect of ZnO nanoparticles on biodegradation and biotransformation of co-substrate and sulphonated azo dye in anaerobic biological sulfate reduction processes. Int Biodeterior Biodegradation 109, 150–156, doi:10.1016/j.ibiod.2016.01.015 (2016).

    CAS  Article  Google Scholar 

  4. 4.

    Yin, W. et al. Functionalized nano-MoS2 with peroxidase catalytic and near-infrared photothermal activities for safe and synergetic wound antibacterial applications. ACS Nano 10, 11000–11011, doi:10.1021/acsnano.6b05810 (2016).

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Rasool, K. et al. Antibacterial activity of Ti3C2Tx MXene. ACS Nano 10, 3674–3684, doi:10.1021/acsnano.6b00181 (2016).

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Liu, C. et al. Rapid water disinfection using vertically aligned MoS2 nanofilms and visible light. Nat Nano 11, 1098–1104, doi:10.1038/nnano.2016.138 (2016).

    CAS  Google Scholar 

  7. 7.

    Huiqiao, W., Jinbin, L., Xuan, W., Zhonghua, T. & Zhaoxiang, D. Tailor-made Au@Ag core–shell nanoparticle 2D arrays on protein-coated graphene oxide with assembly enhanced antibacterial activity. Nanotechnology 24, 205102, doi:10.1088/0957-4484/24/20/205102 (2013).

    Article  Google Scholar 

  8. 8.

    Xu, W.-P. et al. Facile synthesis of silver@graphene oxide nanocomposites and their enhanced antibacterial properties. J Mater Chem 21, 4593–4597, doi:10.1039/c0jm03376f (2011).

    CAS  Article  Google Scholar 

  9. 9.

    Tang, J. et al. Graphene oxide–silver nanocomposite as a highly effective antibacterial agent with species-specific mechanisms. ACS Appl Mater Interfaces 5, 3867–3874, doi:10.1021/am4005495 (2013).

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Li, J. et al. Antibacterial activity of large-area monolayer graphene film manipulated by charge transfer. Sci Rep 4, doi:10.1038/srep04359 (2014).

  11. 11.

    Hu, W. et al. Graphene-based antibacterial paper. ACS Nano 4, 4317–4323, doi:10.1021/nn101097v (2010).

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Akhavan, O. & Ghaderi, E. Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano 4, 5731–5736, doi:10.1021/nn101390x (2010).

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Salas, E. C., Sun, Z., Lüttge, A. & Tour, J. M. Reduction of graphene oxide via bacterial respiration. ACS Nano 4, 4852–4856, doi:10.1021/nn101081t (2010).

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Tu, Y. et al. Destructive extraction of phospholipids from Escherichia coli membranes by graphene nanosheets. Nat Nano 8, 594–601, doi:10.1038/nnano.2013.125 (2013).

    CAS  Article  Google Scholar 

  15. 15.

    Kim, I. Y. et al. Strongly-coupled freestanding hybrid films of graphene and layered Titanate Nanosheets: An Effective Way to Tailor the Physicochemical and Antibacterial properties of graphene Film. Adv Funct Mater 24, 2288–2294, doi:10.1002/adfm.201303040 (2014).

    CAS  Article  Google Scholar 

  16. 16.

    Liu, S. et al. Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. ACS Nano 5, 6971–6980, doi:10.1021/nn202451x (2011).

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Kang, S., Pinault, M., Pfefferle, L. D. & Elimelech, M. Single-walled carbon nanotubes exhibit strong antimicrobial activity. Langmuir 23, 8670–8673, doi:10.1021/la701067r (2007).

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Guo, L. et al. Iron Bioavailability and redox activity in diverse carbon nanotube samples. Chem Mater 19, 3472–3478, doi:10.1021/cm062691p (2007).

    CAS  Article  Google Scholar 

  19. 19.

    Pulskamp, K., Diabaté, S. & Krug, H. F. Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants. Toxicol Lett 168, 58–74, doi:10.1016/j.toxlet.2006.11.001 (2007).

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Narayan, R. J., Berry, C. J. & Brigmon, R. L. Structural and biological properties of carbon nanotube composite films. Mater Sci Eng, B 123, 123–129, doi:10.1016/j.mseb.2005.07.007 (2005).

    Article  Google Scholar 

  21. 21.

    Anasori, B., Lukatskaya, M. R. & Gogotsi, Y. 2D metal carbides and nitrides (MXenes) for energy storage. Nat Rev Mater 2, 16098, doi:10.1038/natrevmats.2016.98 (2017).

    ADS  CAS  Article  Google Scholar 

  22. 22.

    Ying, Y. et al. Two-dimensional titanium carbide for efficiently reductive removal of highly toxic chromium(VI) from water. ACS Appl Mater Interfaces 7, 1795–1803, doi:10.1021/am5074722 (2015).

    CAS  Article  PubMed  Google Scholar 

  23. 23.

    Peng, Q. et al. Unique lead adsorption behavior of activated hydroxyl group in two-dimensional titanium carbide. J Am Chem Soc 136, 4113–4116, doi:10.1021/ja500506k (2014).

    CAS  Article  PubMed  Google Scholar 

  24. 24.

    Ghidiu, M. et al. Ion-exchange and cation solvation reactions in Ti3C2 MXene. Chem. Mater. 28, 3507–3514, doi:10.1021/acs.chemmater.6b01275 (2016).

    CAS  Article  Google Scholar 

  25. 25.

    Mashtalir, O. et al. Dye adsorption and decomposition on two-dimensional titanium carbide in aqueous media. J Mater Chem A 2, 14334–14338, doi:10.1039/c4ta02638a (2014).

    CAS  Article  Google Scholar 

  26. 26.

    Lin, H., Wang, X., Yu, L., Chen, Y. & Shi, J. Two-dimensional ultrathin MXene ceramic nanosheets for photothermal conversion. Nano Letters 17, 384–391, doi:10.1021/acs.nanolett.6b04339 (2017).

    ADS  CAS  Article  PubMed  Google Scholar 

  27. 27.

    Lukatskaya, M. R. et al. Cation intercalation and high volumetric capacitance of two-dimensional titanium carbide. Science 341, 1502–1505, doi:10.1126/science.1241488 (2013).

    ADS  CAS  Article  PubMed  Google Scholar 

  28. 28.

    Ren, C. E. et al. Charge- and size-selective ion sieving through Ti3C2Tx MXene membranes. J Phys Chem Lett 6, 4026–4031, doi:10.1021/acs.jpclett.5b01895 (2015).

    CAS  Article  PubMed  Google Scholar 

  29. 29.

    Ding, L. et al. A two-dimensional lamellar membrane: MXene nanosheet stacks. Angewandte Chemie, doi:10.1002/ange.201609306 (2017).

  30. 30.

    Mahmoud, K. A., Mansoor, B., Mansour, A. & Khraisheh, M. Functional graphene nanosheets: The next generation membranes for water desalination. Desalination 356, 208–225, doi:10.1016/j.desal.2014.10.022 (2015).

    CAS  Article  Google Scholar 

  31. 31.

    Han, Y., Xu, Z. & Gao, C. Ultrathin graphene nanofiltration membrane for water purification. Adv Funct Mater 23, 3693–3700, doi:10.1002/adfm.201202601 (2013).

    CAS  Article  Google Scholar 

  32. 32.

    Lipatov, A. et al. Effect of synthesis on quality, electronic properties and environmental stability of individual Ti3C2 MXene flakes. Adv Electron Mater 2, 1600255, doi:10.1002/aelm.v2.12 (2016).

    Article  Google Scholar 

  33. 33.

    Mashtalir, O. et al. Intercalation and delamination of layered carbides and carbonitrides. Nat Commun 4, 1716, doi:10.1038/ncomms2664 (2013).

    Article  PubMed  Google Scholar 

  34. 34.

    Ren, C. E. et al. Porous Two-dimensional transition metal carbide (MXene) flakes for high-performance Li-Ion storage. Chem Electro Chem 3, 689–693, doi:10.1002/celc.201600059 (2016).

    CAS  Google Scholar 

  35. 35.

    Ma, W., Zhang, J., Wang, X. & Wang, S. Effect of PMMA on crystallization behavior and hydrophilicity of poly(vinylidene fluoride)/poly(methyl methacrylate) blend prepared in semi-dilute solutions. Appl Surf Sci 253, 8377–8388, doi:10.1016/j.apsusc.2007.04.001 (2007).

    ADS  CAS  Article  Google Scholar 

  36. 36.

    Naguib, M. et al. One-step synthesis of nanocrystalline transition metal oxides on thin sheets of disordered graphitic carbon by oxidation of MXenes. Chem Commun 50, 7420–7423, doi:10.1039/c4cc01646g (2014).

    CAS  Article  Google Scholar 

  37. 37.

    Peng, C., Wang, H., Yu, H. & Peng, F. (111) TiO2−x/Ti3C2: Synergy of active facets, interfacial charge transfer and Ti3+ doping for enhance photocatalytic activity. Mater Res Bull 89, 16–25, doi:10.1016/j.materresbull.2016.12.049 (2017).

    CAS  Article  Google Scholar 

  38. 38.

    Gao, Y. et al. Novel hierarchical TiO2/C nanocomposite with enhanced photocatalytic performance. Nano 10, 1550064, doi:10.1142/s1793292015500642 (2015).

    CAS  Article  Google Scholar 

  39. 39.

    Zhu, J., Tang, Y., Yang, C., Wang, F. & Cao, M. Composites of TiO2 nanoparticles deposited on Ti3C2 MXene nanosheets with enhanced electrochemical performance. J Electrochem Soc 163, A785–A791, doi:10.1149/2.0981605jes (2016).

    CAS  Article  Google Scholar 

  40. 40.

    Ghassemi, H. et al. In situ environmental transmission electron microscopy study of oxidation of two-dimensional Ti3C2 and formation of carbon-supported TiO2. J Mater Chem A 2, 14339–14343, doi:10.1039/c4ta02583k (2014).

    CAS  Article  Google Scholar 

  41. 41.

    Halim, J. et al. X-ray photoelectron spectroscopy of select multi-layered transition metal carbides (MXenes). Appl Surf Sci 362, 406–417, doi:10.1016/j.apsusc.2015.11.089 (2016).

    ADS  CAS  Article  Google Scholar 

  42. 42.

    Seddiki, O., Harnagea, C., Levesque, L., Mantovani, D. & Rosei, F. Evidence of antibacterial activity on titanium surfaces through nanotextures. Appl Surf Sci 308, 275–284, doi:10.1016/j.apsusc.2014.04.155 (2014).

    ADS  CAS  Article  Google Scholar 

  43. 43.

    Turner, R. D., Hurd, A. F., Cadby, A., Hobbs, J. K. & Foster, S. J. Cell wall elongation mode in Gram-negative bacteria is determined by peptidoglycan architecture. Nat Commun 4, 1496, doi:10.1038/ncomms2503 (2013).

    ADS  Article  PubMed  PubMed Central  Google Scholar 

  44. 44.

    Hayhurst, E. J., Kailas, L., Hobbs, J. K. & Foster, S. J. Cell wall peptidoglycan architecture in Bacillus subtilis. Proc Natl Acad Sci 105, 14603–14608, doi:10.1073/pnas.0804138105 (2008).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  45. 45.

    Chick, H. An investigation of the laws of disinfection. J Hyg 8, 92–158, doi:10.1017/S0022172400006987 (1908).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  46. 46.

    O’Brien-Simpson, N. M., Pantarat, N., Attard, T. J., Walsh, K. A. & Reynolds, E. C. A. A Rapid and Quantitative flow cytometry method for the analysis of membrane disruptive antimicrobial activity. PLOS ONE 11, e0151694, doi:10.1371/journal.pone.0151694 (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  47. 47.

    Zhang, C. et al. Layered Orthorhombic Nb2O5@Nb4C3Tx and TiO2@Ti3C2Tx Hierarchical composites for high performance Li-ion batteries. Adv Funct Mater 26, 4143–4151, doi:10.1002/adfm.201600682 (2016).

    CAS  Article  Google Scholar 

  48. 48.

    Perreault, F. et al. Biofouling mitigation in forward osmosis using graphene oxide functionalized thin-film composite membranes. Environ Sci Technol 50, 5840–5848, doi:10.1021/acs.est.5b06364 (2016).

    ADS  CAS  Article  PubMed  Google Scholar 

  49. 49.

    Musico, Y. L. F., Santos, C. M., Dalida, M. L. P. & Rodrigues, D. F. Surface modification of membrane filters using graphene and graphene oxide-based nanomaterials for bacterial inactivation and removal. ACS Sustain Chem Eng 2, 1559–1565, doi:10.1021/sc500044p (2014).

    CAS  Article  Google Scholar 

  50. 50.

    Sang, X. et al. Atomic defects in monolayer titanium carbide (Ti3C2Tx) MXene. ACS Nano 10, 9193–9200, doi:10.1021/acsnano.6b05240 (2016).

    CAS  Article  Google Scholar 

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The authors are grateful to the financial support from Qatar Foundation. This report was also made possible by financial support from NPRP grant (NPRP9-254-2-120) from the Qatar National Research Fund (a member of Qatar Foundation). The findings achieved herein are solely the responsibility of the authors. Multilayer MXene was provided by Chang Ren, Drexel Nanomaterials Institute. FESEM analysis was performed at the Central Lab Unit, Qatar University. The authors are grateful to Department of Biological and Environmental Sciences, Qatar University for their support and providing bacteria strains.

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K.M. and K.R. conceived the research idea and performed the experimental work. D.J. conducted the AFM analysis, performed calculation and interpreted the results. M.H. performed and discussed the SEM analysis. G.R.B. contributed to data analysis and illustration. Y.G. discussed the results and provided guidance of experimental steps. K.R., K.M., D.J. and Y.G., wrote the manuscript. All authors contributed to the discussion of the results and edited the manuscript.

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Correspondence to Khaled A. Mahmoud.

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Rasool, K., Mahmoud, K.A., Johnson, D.J. et al. Efficient Antibacterial Membrane based on Two-Dimensional Ti3C2Tx (MXene) Nanosheets. Sci Rep 7, 1598 (2017). https://doi.org/10.1038/s41598-017-01714-3

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