Figure 5 | Scientific Reports

Figure 5

From: N-Glycan Modification of a Recombinant Protein via Coexpression of Human Glycosyltransferases in Silkworm Pupae

Figure 5

Expression of recombinant proteins in silkworm pupae with coexpression of hGnT II and hGalT I. (A) Expression of hGalT I in silkworm pupae by using BmNPV CPChi/PAct-hGalT I or BmNPV CPChi/PPol-hGalT I. Pupae were homogenized with TBST and centrifuged to separate the supernatant from the insoluble material. Western blotting was performed on both fractions. Lane 1: Supernatant, lane 2: Pellet. (B) SDS-PAGE of purified hIgG coexpressed with PPol-hGnT II and PPol-hGal T I (left panel), PPol-hGnT II and PAct-hGal T I (middle panel) and PAct-hGnT II and PPol-hGal T I (right panel). Gels were stained with CBB.

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