Cnot3 overexpression induces human cardiomyocyte proliferation. (a) Representative images of immunofluorescence staining against Ki67 and Troponin T2 (TnnT2) to show the impacts of Cnot3 overexpression on the proliferation of hESC-derived cardiomyocytes. (b) Percentage of Ki67 positive cells was counted, and plotted as mean ± SEM from ten images from three independent experiments, *p < 0.05. (c) Ki67 positive nuclei were counted from ten images from three independent experiments, and significance was calculated by chi-square test, *p < 0.05. (d) qPCR to show the expression of CDKI mRNA 96 hours after Cnot3 overexpression in hESC-derived cardiomyocytes. β-actin was used as endogenous control and values were plotted as mean ± SEM from three independent experiments. (e) Western blot to show the Cnot3 protein levels in human heart tissues from adult versus infant. β-actin was used as loading control. The blots were cropped since different antibodies were incubated at the same time.