Figure 2 | Scientific Reports

Figure 2

From: Cnot3 enhances human embryonic cardiomyocyte proliferation by promoting cell cycle inhibitor mRNA degradation

Figure 2

Cnot3 plays an important role in cardiomyocyte proliferation. (a,b) hESCs were induced to differentiate into cardiomyocytes by EB suspension culture. The expression of Cnot3 was determined by western blotting (a) and qPCR (b) at the indicated time points, respectively. β-actin was used as loading control in western blot and endogenous control in RT-PCR. The blots were cropped since different antibodies were incubated at the same time. (c,d) hESCs were induced to differentiate into cardiomyocytes by monolayer culture. The expression of Cnot3 was determined by both western blotting (c) and qPCR (d) at the indicated time points. β-actin was used as loading control in western blot and endogenous control in RT-PCR, respectively. (e) Representative images of immunofluorescence staining against Ki67 and Troponin T2 (TnnT2) to show the impacts of Cnot3 depletion on the proliferation of hESC-derived cardiomyocytes. (f) Ki67 positive cells were counted, and percentage of Ki67-positive cells was calculated and plotted as mean ± SEM from ten images from three independent experiments, *p < 0.05. (g) Ki67 positive nuclei were counted from ten images from three independent experiments, and significance was calculated by chi-square test, *p < 0.05.

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