Figure 4 | Scientific Reports

Figure 4

From: A deep intronic CLRN1 (USH3A) founder mutation generates an aberrant exon and underlies severe Usher syndrome on the Arabian Peninsula

Figure 4

Quantification of the RT-PCR results shown in Fig. 3C. (A) Quantification of eight RT-PCR experiments resulting from four independent transfection experiments for both WT and c.254–649T > G mutant for the 432 bp and 662 bp bands. The single values are given as percentages of the total band intensities ± standard error of the mean (SEM). (B) Quantification of the single WT and the c.254–649T > G cDNAs used for the calculation of band intensities displayed in (A). Shown are the mean CT values (n = 3) ± SEM for aminolevulinic acid synthase (ALAS) as housekeeper gene. The single values are summarized in Table 1. For statistical analysis, one-way ANOVA followed by Tukeys test was used. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Table 1 Mean and SEM values of the data displayed in Fig. 4.

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