Subsets of “house-keeping” proteins confirmed findings for entire proteomes. We reduced the analysis to SAVs from subsets of orthologs between three organisms (human, chimp, mouse), and with SAVs observed in the 1KG data. For brevity, we referred to those as to “house-keeping” proteins. With respect to the observation for the entire data set (Fig. 1), the curves shifted less strongly, but the main trend remained: a higher fraction of the SAVs in cross-species comparison (human-chimp and human-mouse) was predicted as neutral than for the SAVs between healthy individuals (1KG). Furthermore, the shift between cross-species and 1KG was higher for larger evolutionary distances (more neutral for larger distance).