The new media preserves normal cell function. (a) Comparison of the effect of DMEM + SATO and MEMO + SATO based media upon OPC migration over 4 days from agarose drop (Brightfield images), OPC proliferation using NG2 and Ki67 staining after 3 days with mitogens PDGF-aa and FGF-b (middle panel) and OPC differentiation with terminal differentiation marker MBP after 5 days of mitogen withdrawal and treatment with T3 (right panel). (b) Data from (a) analyzed by average distances from edge of drop to corona of migrated cells, percentages of NG2+ cells with Ki67+ or EdU+ and percentages of MBP+ cells and percentage MBP area generated for differentiation, and all normalized to DMEM + SATO values with data representing means ± s.e.m. of a number of biological replicates (value within or above each histogram) and p-values from Student’s two tailed unequal variance t-test. (c) Comparison of neural epithelial cell differentiation from human iPSC generated embryonic bodies using serum free supplements N2+B27 (left hand panels) or SOS (right hand panels) to generate neurons (β-III) and astrocytes (GFAP) after approximately 5 weeks in vitro.