Figure 4 | Scientific Reports

Figure 4

From: Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink

Figure 4

Three-dimensional-bioprinted cartilage-like tissue from iPSCs excluding iChons. (A and B) Histology sections of 3D-bioprinted constructs at week 6 (week 1 proliferation in conditioned DEF medium for the iPSCs plus 5 weeks of chondrogenic differentiation with TGFβ1, TGFβ3, GDF5 and BMP2) (stained with Alcian blue-van Gieson for proteoglycans/glycosaminoglycans (GAGs) (blue) and nuclei (brown)) (the scale bars represent 500 μm in A and 100 μm in B). (C) Cell survival of single iPSCs in the DEF medium or in the iPSC-conditioned DEF medium.

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