LOX/L expression in experimental lung fibrosis. (a) Masson trichrome stained histological sections of control (NaCl) or Bleomycin treated animals, 14 days after Bleomycin instillation. Nuclei appear in dark red, cytoplasm in light red and collagen in blue. (b) LOX/L gene expression was determined in whole lung homogenates from Bleomycin treated mice at day 14 after treatment using Taqman gene expression assays. Data is normalized to RNA-polymerase 2 expression (Ct value of 25) and presented as mean fold change ± SD of 8–9 animals per group. *p < 0.05; **p < 0.01; ***p < 0.001, relative to control treatment. (c) Representative images of immunohistochemical analyses of LOX, LOXL1, LOXL2, LOXL3 and LOXL4 at day 14 after intratracheal Bleomycin instillation. Positive cells are depicted in brown. (d) Correlation of the mRNA results shown in (b) with computational IHC image analysis-derived fold changes in protein expression (see Supplemental Fig. S2 for details).