Functional trait data enhance climate change research by linking climate change, biodiversity response, and ecosystem functioning, and by enabling comparison between systems sharing few taxa. Across four sites along a 3000–4130 m a.s.l. gradient spanning 5.3 °C in growing season temperature in Mt. Gongga, Sichuan, China, we collected plant functional trait and vegetation data from control plots, open top chambers (OTCs), and reciprocally transplanted vegetation turfs. Over five years, we recorded vascular plant composition in 140 experimental treatment and control plots. We collected trait data associated with plant resource use, growth, and life history strategies (leaf area, leaf thickness, specific leaf area, leaf dry matter content, leaf C, N and P content and C and N isotopes) from local populations and from experimental treatments. The database consists of 6,671 plant records and 36,743 trait measurements (increasing the trait data coverage of the regional flora by 500%) covering 11 traits and 193 plant taxa (ca. 50% of which have no previous published trait data) across 37 families.
|Measurement(s)||leaf growth and development trait • Abundance • leaf area trait • leaf composition trait • leaf morphology trait • Vascular Plant • total biomass yield • climate|
|Technology Type(s)||balance • visual observation method • Scanner Device • leaf stoichiometry and isotope assays • weather station|
|Factor Type(s)||plants • temperature • plant functional traits (specific leaf area, leaf dry matter content, leaf carbon content, leaf nitrogen content, leaf size, leaf thickness) • type of treatment (e.g in situ warming experiments using an Open Top Chamber)|
|Sample Characteristic - Organism||Tracheophyta|
|Sample Characteristic - Environment||area of sedge- and forb-dominated herbaceous vegetation|
|Sample Characteristic - Location||Ganzi Tibetan Autonomous Prefecture|
Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.12033786
Background & Summary
Climate warming has wide-ranging impacts on biodiversity and functioning of alpine ecosystems, affecting phenology1,2, species ranges3,4, local plant abundance and biodiversity5,6, and ecosystem carbon, nutrient, and water fluxes7. However, there is also substantial variation between systems and regions in magnitude and directions of the response to a given climate change8,9,10. These context-dependencies limit our understanding of observed climate change impacts and our ability to forecast future trends in biodiversity and ecosystem functioning11,12. Comparisons across sites or regions, either through formalized replicated experiments or meta-analyses and other types of syntheses, are critically important to understand what drives the variation in biodiversity and ecosystem response to climate change.
Trait-based approaches offer opportunities for generalization and improved process-based understanding in global change ecology; and in particular for understanding drivers and consequences of context-dependencies in climate responses. Functional traits underlie variation among individuals in their ability to survive, reproduce, and function under different environmental conditions13,14,15. Because traits vary both within and between species, and can be affected by environment, evolutionary history and plasticity16,17, trait-based approaches offer great opportunity for improved understanding of the drivers, constraints and consequences of variability in biodiversity and ecosystem responses to climate change. In alpine regions, where temperature is a major limiting factor, traits associated with the leaf economics spectrum, a set of leaf traits that characterize individuals along a continuum from ‘fast’ to ‘slow’ photosynthetic and tissue turnover rates and life histories18,19,20, should be particularly relevant for responses to climatic warming. Functional traits thus improve our mechanistic understanding of species’ response to and functioning under climate change by providing a linkage between the phenotypes of individuals and the environment. An advantage of a trait-based approach is that trait measures offer a ‘common currency’ that facilitates comparison across regions and systems that may not share many taxa, as well as across experimental approaches.
Impacts of climate warming on plant communities are studied through four main empirical approaches. First, climate effects are assessed along natural elevation gradients, assuming a space-for-time substitution21,22. Second, ongoing climate change is studied through resampling of natural communities over time6,23. A third approach relies on on-site experimental warming, for example by Open Top Chambers (OTCs)8,24. A fourth approach is to transplant whole communities to warmer and/or cooler climates25,26,27. Each method has strengths and weaknesses, for example, gradient and resampling studies allow large-scale and long-term comparisons, OTCs experimentally expose intact extant communities to new climates, whereas community transplants manipulate both the biotic and the abiotic environment thus exploring the net effect of both direct and indirect climate change impacts28,29. Combining different approaches within a single study or system can be a powerful way to exploit these complementary strengths.
In this paper, we report a unique plant functional traits dataset collected from a combined OTC and transplant experiment along an 1100 m elevational gradient from 3000 to 4130 m above sea level (a.s.l.) in Mt. Gongga, Hengduan Mountains, Sichuan Province, China; referred to as «Mt. Gongga, China» below. Across four study sites we conducted an elevation gradient study, in situ warming experiments using OTCs, and reciprocal community transplantation experiments (warming and cooling) between all pairs of neighbouring sites and, as an extreme treatment, between the highest and lowest sites (extreme warming and cooling). In all three approaches, plant species richness, cover and associated climate data were measured each year from 2012 to 201629. Plant functional traits of the vascular plant community were collected from the local grassland flora of each site in 2015 and 2016, and from the experimental treatments in 2016. The resulting datasets encompass vegetation and climate data and 36,743 trait measurements from 193 taxa, which extends existing trait data from the regional flora by ca. 100 additional species, and increases the number of unique trait measurements from this regional flora by 500%, according to the Botanical Information and Ecology Network (BIEN; http://bien.nceas.ucsb.edu/bien/) database30. These traits campaigns were conducted as part of two Plant Functional Traits Courses (PFTC1 and PFTC2) for international students in trait-based theory and methods (see also31,32). The data are comparable with data from later courses in Wayquecha, Peru (PFTC3 and 5) in 2018 and 2020 and Longyearbyen, Svalbard (PFTC4) in 2018 as well as with upcoming courses.
Research site selection and basic site information
The study was conducted in the Hengduan Mountains, Sichuan Province, China (Fig. 1). This area is on the south-eastern edge of the Tibetan Plateau, and the study sites are located in Kang-Ding Valley, on the northwest slopes of Mt. Gongga along a steep elevational gradient where the lower part is characterized by mixed coniferous-broadleaved forest, followed by belts of subalpine coniferous forest, sub-alpine meadows, subalpine shrub, and alpine meadows towards higher elevations and colder climates33,34.
In 2012, four study sites along the slope were chosen to reflect the major bioclimatic variation in southwest mountain grassland vegetation along an elevation gradient of 1100 meters; the Lowland site at 3000 m a.s.l, the Middle sites at 3500 m a.s.l., the Alpine site at 3850 m a.s.l., and the High Alpine site at 4130 m a.s.l. (Fig. 1, Table 1). All the sites were placed selectively in grasslands associated with mountain gray-brown soil originating from granite35. The sites were selected to represent the charactieristic grasslands at different elevations and hence climatic conditions, but to otherwise be as similar as possible with respect to environmental conditions, vegetation structure, plant community composition, slope, aspect, etc. The geographical distance between adjacent sites is on average ca 2 km.
At each site, we selected an experimental area within the grassland as homogeneous and representative of the grasslands at that elevation as possible. The experimental areas were placed on sloping ground, avoiding depressions and concave areas in the landscape and other features such as big rocks or formations that may affect microclimate, light conditions, hydrology and/or snowdrift. All sites were moderately grazed prior to the experiment by yak, sheep, cattle, goats, and/or horses, and the experimental areas were fenced and locked for the duration of the study to prevent grazing and human disturbance of the experimental infrastructure. The fenced area was mowed at the end of each growing season to mimic past grazing regimes and minimize fence effects.
Block and plot setup
Within each of the four fenced experimental areas, we established seven replicate blocks, with a distance between the blocks ranging from four to six meters. Blocks were selectively placed in homogenous grassland, avoiding rocks, depressions, and other features as described above. Each block comprised five 25 cm × 25 cm plots, which were placed in a regular 3 rows x 2 columns grid (facing uphill) with 50 cm between adjacent plots. If a plot contained more than 10% bare rock, shrubs, or other non-grassland features, they were rejected or moved slightly to avoid these features. The plots were numbered by row from the uphill left corner of each block, permanently marked with plastic poles in each corner, onto which a standardized vegetation analysis frame could be mounted for vegetation (re)sampling. Within sites, the experimental area containing all experimental blocks is situated within a total area of ca. 75–200 m2.
In 2015, 73 additional 50 cm × 50 cm plots were set up within the fenced block areas in between the experimental blocks for biomass sampling (see dataset iii).
Transplant and Open Top Chamber) experiments
Within each of the seven blocks at each site, the pre-established and numbered plots were randomly designated to the following experimental treatments (with the specific treatments depending on the site, Fig. 1): (1) passive warming with an Open Top Chamber (OTC), (2) transplanting to a site one step warmer along the gradient (treatment ‘Warming’; from sites High alpine, Alpine, Middle), (3) transplanting to a site one step colder along the gradient (‘Cooling’; from sites Alpine, Middle, Lowland), (4) transplanting down the entire gradient (‘Extreme warming’; from site High alpine), and (5) up the entire gradient (‘Extreme cooling’, from site Lowland), (6) transplanting within blocks (to control for the transplanting itself)(‘Local transplant’; all sites), and (7) an untouched control plot (‘Control’; all sites). Thus, each OTC has a local unmanipulated control, and each transplanted turf has an “origin” site and a “destination” site, each with two types of controls, a local transplant and an untouched control plot (Fig. 1). The OTC chambers are hexagonal, 40 cm tall, with a distance between parallel sides of 106 cm at the base and 60 cm at the top. Snow cover is low and scattered at all sites, and the OTCs were left out year-round to achieve warming during the entire snow-free period. Because of the low snow cover, removing OTCs was also not necessary for their protection against snow damage.
We marked the upslope centre of each plot with a plastic flag to ensure that all plots were always analysed from the same direction, and that transplanted turfs retained their orientation relative to the slope and block layout at the destination site. For the transplant treatments, we used a knife to cut the turfs 2 cm outside the plot margins, giving turfs of 29 cm × 29 cm and to a depth of 20 cm, unless the organic soil was shallower, as was the case for some of the higher-elevation plots. After excavation, the turfs were packed into 29 cm × 29 cm waterproof boxes and transported to their respective destination sites within one or two days. To keep the transplant disturbance as similar as possible among treatments, the excavated control (‘home transplant’) turfs were also kept in boxes before they were put into their designated randomized destination plots within the site of origin.
The transplanted turfs were fitted into gaps created by excavating turfs at the destination site. Each block received one plot of each relevant treatment, resulting in five turfs per block, but with different specific treatments in the two middle sites and the extremes of the gradient (see Fig. 1). Transplanted turf positions were randomized within the destination blocks, while un-transplanted control and OTC warming plots - were not moved. Transplanted turfs were then carefully planted into their destination plots ensuring that the original orientation of the turf vs. the block slope was retained, the soil surface was level with the surrounding soil surface, and the edges of the excavated plot were in good contact with the sides of the gap. If necessary, loose soil was carefully removed from the underside of the turf, or local soil was added to the gap or around the edges to achieve this. This experimental design had a total of 140 plots. In 2014, two replicate blocks (10 plots) in the High alpine site were damaged by yaks, so having only five blocks in this site going forward.
Species identification, taxonomy, and flora
All species sampled in the vegetation and functional trait datasets were identified in the field. Back at the field station, the identification of sampled whole plants or vouchers were checked by a taxonomic expert from the region using the Online Flora of China36. Specimens problematic to identify were brought back to the Chongqing Normal University for identification and deposition of vouchers by one of the co-authors (Prof. He). Forbs were identified to species level, whereas many of the graminoids were identified only to genus level, i.e., Carex spp., Poa spp., Kobresia spp., and Festuca spp., due to difficulties with identification of sterile graminoids as there may be undescribed taxa and as there are no keys for vegetative plants for this region (professor He, personal observation). All taxon names were standardized using the Taxonomic Name Resolution Service37.
Dataset collection methods
Dataset (i): Plant community composition sampling
All vascular plant species in each plot were surveyed in 2012 (before treatment), and annually between 2013 and 2016. Each year, vegetation was surveyed during the peak of the growing season using a 25 cm × 25 cm frame overlain with a grid of 5 cm × 5 cm subplots. Subplots were numbered 1–25 starting from the up-slope left-hand corner of the plot, numbering the subplots from left to right by rows. We registered presence-absence of all species in each subplot, and estimated the percentage coverage of each species in the whole plot to the nearest 1%. Note that the total coverage in each plot can exceed 100, due to layering of the vegetation.
Dataset (ii): Vegetation height and structure sampling
Vegetation structure data for each plot was recorded between 2012 and 2016. Mean vegetation height and bryophyte depth was measured at five evenly spaced points per plot using a ruler. The total percent coverage of all vascular plants combined, was also recorded.
Dataset (iii): Biomass sampling
We measured the standing biomass per vascular plant species in the 73 additional 50 cm × 50 cm plots placed out in the general experimental area of each site in 2015. For each plot, species composition was registered, percentage cover of each species estimated using a 50 cm × 50 cm frame with 10 cm × 10 cm overlay, and the height measured at five positions using a ruler. The plot was then harvested at the ground level, sorted to species, and the biomass dried at 65 °C for 3 days before weighing to the nearest 0.0001 g. As the aboveground grass and forb biomass dies back each winter, standing biomass can be considered an approximation of aboveground net primary productivity in these grasslands.
Dataset (iv): Trait sampling and lab analyses
Site-level sampling for leaf trait analyses. We collected whole plants for leaf trait analyses from the common species in the plant community at each of the four sites in August 2015 and 2016. In 2015, we sampled as many species as possible from each of the four sites. In 2016, the collection was complemented to ensure that we had trait data from species making up at least 80% of the vegetation cover in all control plots at each site. The plants were collected outside of the experimental plots within a 50 m perimeter from the blocks, and we aimed to collect up to five individuals from each species in each site. To avoid repeated sampling from a single clone, we selected individuals that were visibly separated from other ramets of that species. The sampled plant individuals were labelled, put in plastic bags with moist paper towels, and stored in darkness at 4 °C until further processing. Processing was done as soon as possible, but due to unexpected high diversity in the grasslands and therefore many collected plants, some specimens were stored for longer than optimal, in 2015 for up to 4 days and in 2016 for up to 2 days. As a result, some of the site-level samples, especially from 2015, were less well hydrated/more wilted and/or generally in a poorer state than what is optimal for trait measurements (see below for discussion of data documentation and quality checking; note that the raw leaf scans are available for all leaves). Before processing, plant identification was checked vs. the Online Flora of China36. Up to three healthy, fully expanded leaves were then sampled from each individual. The leaves were cut off as close to the stem as possible, including the blade, petiole, and stipules when present. Further processing was completed within 24 hours (see below).
Experimental treatment-level leaf sampling for trait analyses
Ten of the most common species along the gradient were selected for more intensive sampling within the experimental treatments plots in August 2016. We targeted species (i) with broad distributions along the gradient, (ii) that were also locally frequent and therefore present in a number of turfs prior to the experiments, and (iii) that remained present in most treatments until the sampling year. This was to ensure sufficient replication within species across sites and treatments. Further, to prevent measuring individuals that had colonized the sampled plots during the course of the experiment, and therefore had not been subjected to the experimental treatments, we (iv) excluded species that spread fast clonally. The selected species for treatment-level analysis are Artemisia flaccida, Epilobium fangii, Geranium pylzowianum, Hypericum wrightianum, Pedicularis davidii, Polygonum viviparum (Persicaria vivipara), Plantago asiatica, Potentilla leuconota, Veronica szechuanica, and Viola biflora var. rockiana. Of these species, P. leuconota and V. szechuanica were present across the whole elevation gradient. All other species were sampled from all treatments at sites where they are naturally occurring, including from transplanted turfs outside of the natural elevation range. Because many species in this system display at least some clonal reproduction, distinguishing genetic individuals is impossible without destructive sampling, and we therefore work at the ramet level, following38. For all sites and selected species, we collected up to five healthy, fully expanded leaves from up to five ramets in all experimental plots (i.e., control, locally transplanted control, moderate and extreme warmed and cooled transplants, open top chamber) where that species occurred. The leaves were cut off as close to the stem as possible, including the blade, petiole, and stipules when present. The leaves were labelled, put in plastic bags with moist paper towels, and stored dark at 4 °C and further processed within 24 hours of collection.
Plant functional trait measurements
We measured 11 leaf functional traits that are related to potential physiological growth rates and environmental tolerance of plants, following the standardized protocols in Pérez-Harguindeguy et al.39: leaf area (LA, cm2), leaf thickness (LT, mm), leaf dry matter content (LDMC, g/g), specific leaf area (SLA, cm2/g), carbon (C, %), nitrogen (N, %), phosphorus (P, %), carbon nitrogen ratio (C:N), nitrogen phosphorus ratio (N:P), carbon13 isotope ratio (δ13C, ‰), and nitrogen15 isotope ratio (δ15N, ‰).
Initial leaf processing was done at the field station of the Institute for Mountain Hazards and Environment (IMHE) in Moxi, Sichuan. Processing was done in the following steps:
Leaf area. Leaves (including blade, petiole, and stipules when present) were carefully patted dry with paper towels, flattened (folded out to their maximum area) and scanned using a Canon LiDE 220 flatbed scanner at 300 dpi. Care was taken that no leaf parts were overlapping on the scanner, and naturally overlapping parts of lamina (e.g., as is the case in some compound leaves) were cut off and placed next to each other on the scanner to obtain the full leaf area. Leaves that could not be flattened because they are narrow and grow naturally folded (e.g., some Festuca sp.) were scanned as they were, thereafter the area was multiplied by 2 during data processing. Any dark edges on the scans were manually cropped. Leaf area was calculated using ImageJ40 and the LeafArea package41.
Leaf wet mass. Each leaf (including blade, petiole, and stipules when present) was weighted to the nearest 0.001 g to assess fresh mass.
Leaf thickness. Leaf thickness was measured at three locations on each leaf blade with a digital calliper (Micromar 40 EWR, Mahr) and the average was calculated for further analysis. When possible, the three measurements were taken on the middle vein of the leaf and on lamina with and without veins. The petiole or stipule thickness is not measured.
Leaf dry mass. Leaves (including blade, petiole, and stipules when present) were then dried for a minimum of 72 hours at 65 °C before dry mass was measured to the nearest 0.0001 g.
Leaf stoichiometry and isotopes. A subset of leaves (n = 576; 265 from the gradient sampling and 311 from the experiments) were transported to the University of Arizona for leaf stoichiometry and isotope assays (P, N, C, δ15N, and δ13C). The leaves were stored in a drying oven at 65 °C before shipping and processing. Each leaf (including blade, petiole, and stipules when present) was ground into a fine homogenous powder. Total phosphorus concentration was determined using persulfate oxidation followed by the acid molybdate technique (APHA 1992) and phosphorus concentration was then measured colorimetrically with a spectrophotometer (ThermoScientific Genesys20, USA). Nitrogen, carbon, stable nitrogen (δ15N) and carbon (δ13C) isotopes were measured by the Department of Geosciences Environmental Isotope Laboratory at the University of Arizona on a continuous-flow gas-ratio mass spectrometer (Finnigan Delta PlusXL) coupled to an elemental analyzer (Costech). Samples of 1.0 ± 0.2 mg were combusted in the elemental analyser. Standardization is based on acetanilide for elemental concentration, NBS-22 and USGS-24 for δ13C, and IAEA-N-1 and IAEA-N-2 for δ15N. Precision is at least ± 0.2 for δ15N (1 s), based on repeated internal standards. Ratios between C:N and N:P were calculated and used in the analysis.
Datasets (v-vii) – climate data
Climate stations (U30-NRC, Tempcon Instrumentation LTD, UK) at each site recorded precipitation, air temperature and humidity at height of 2 m above ground, and soil moisture were measured at depths of 0 cm, 5 cm and 20 cm below ground in 10 min intervals, starting in September 2012 and continuing during the whole study period. The same measurements were conducted inside OTCs except that here air temperature was measured at 20 cm above ground. Additionally, iButtons (iButtonLink Technologies, USA) were installed at 5 cm below ground, ground level, and 30 cm above ground inside and outside OTCs in each site along the gradient from June–August 2017, at 10 min intervals. Finally, TomsT TMS-4 loggers42 were installed inside and outside OTCs at the High alpine site from September to November 2019. These measure temperatures at 15 cm above ground, ground level, and 6 cm below ground, and soil moisture, at 15 min intervals.
This paper reports on data from field experiments on climate change impacts on alpine grasslands of Mt. Gongga in the Hengduan Mountains, Sichuan Province, China conducted from the 2013 growing season onwards. It consists of plant community and climate observations collected from 2012 (pre-treatment year) through 2016, biomass data from 2015, vegetation height from 2013 and plant trait data sampled and analysed in 2015 and 2016. Data outputs consists of seven datasets, the (i) species composition of the experimental plots, (ii) vegetation height and structure of the experimental plots, (iii) biomass harvested in an additional set of control plots at each site, (iv) plant functional traits of individuals sampled from the sites along the gradient and from the experiments, (v) climate and environmental data of the sites and/or treatments, (vi) iButton temperature data from controls and OTCs at all sites, and (vii) TomsT logger temperature and soil moisture data from controls and OTCs at the High alpine site (Table 2). The structure and relationship between datasets are described in Fig. 2. These data were checked and cleaned according to the procedures described under Technical validation before final data files and associated metadata were produced.
The final data files (see Table 2 for an overview) and all raw data, including leaf scans, are available at Open Science Framework (OSF)43, and the code necessary to access the raw data and produce these cleaned datasets, along with a readme file that explains the various data cleaning steps, issues, and outcomes, are available in an open GitHub repository, with a versioned copy archived in Zenodo44. Note that although parts of these data have previously been made available in connection with earlier publications29,31,38, the files reported in the current paper and associated OSF project contain the complete and final datasets. The reader is referred to the code and the detailed coding, data cleaning, and data accuracy comments and the associated raw and cleaned data and metadata tables for detailed information about the data cleaning process.The Usage notes section in this paper summarises the data accuracy and data cleaning procedures, including caveats regarding data quality and our advice on ‘best practice’ data usage.
Dataset (i): Plant community composition
The plot-level plant community dataset (available in43, where the final cleaned dataset is found in the ‘Community’ folder) has a total of 118 taxa and 9,770 observations (taxa x plots x years) (Tables 2 and 3). This dataset is located in the turfCommunity table in the vegetation database (Fig. 2). For details on the clean dataset and the code to clean and extract these data from the raw data, see Table 2. Mean species richness per plot and year (mean ± SE) is 14.6 ± 0.17 species overall and is decreasing from 16.5 ± 0.27 at the High alpine site via 16.2 ± 0.27 at the Alpine site and 15.8 ± 0.27 at the Middle site to 9.9 ± 0.27 at the Lowland site (Fig. 3). In contrast, only weak patterns with elevation exist for evenness, sum of covers, and proportion graminoids along the gradient (Fig. 3).
The experimental treatments strongly influence all of these community metrics (Fig. 3). Richness and evenness generally increase with cooling and decrease with warming, but with context-dependencies with respect to the type of treatment (OTC vs. transplant and warming vs cooling), position along the gradient, and the severity of the transplant treatment (transplant vs extreme transplant). Sum of covers and proportion graminoids are even more variable.
An NMDS ordination of the full plant community dataset (not shown, but code available in44) illustrate shifts in the plant community composition with elevation and experimental treatments. Transplant communities generally converge towards their respective destination warmer-climate or colder-climate communities, with extreme transplant communities changing faster than adjacent-site transplants. OTC communities change slower than the transplant communities change, and they generally do not converge towards their destination warmer-climate communities. There is also community turnover in the local transplants and controls, but this is not directional over time.
Dataset (ii): Vegetation height and structure
Vegetation height and other structural variables (available in43, where the final cleaned dataset is found in the ‘Community’ folder) are located in the turfEnvironment table in the vegetation database (Tables 2, 4 and Fig. 2). For details on the clean dataset and the code to clean and extract these data from the raw data, see Table 2. Vegetation height after 1 year of treatment increased in the OTCs compared to the control plots by (mean ± SE) 3.01 ± 0.19 cm in the High alpine, 4.21 ± 0.26 cm in the Alpine, 8.58 ± 0.89 cm in the Middle, and 8.22 ± 1.13 cm in the Lowland site (F1,54 = 5.75, P = 0.02) (Table 2, Fig. 3).
Dataset (iii): Above-ground biomass
The above-ground biomass dataset (available in43, where the final cleaned dataset is found in the ‘Biomass’ folder) is based on a sample of 20 plots per site, except from the High alpine site, where we sampled only 13 plots (Tables 2 and 5). Biomass is reported per species per 50 cm × 50 cm plot. Total plot biomass summed over all species increases toward lower elevations along the gradient, with a peak in the Middle site (F3,69 = 19.02, P < 0.001; Table 1). For details on the clean dataset and the code to clean and extract these data from the raw data, see Table 2.
Dataset (iv): Plant functional traits
From all these leaves, leaf physical or structural traits (leaf area, leaf thickness, specific leaf area [SLA], and leaf dry matter content [LDMC]) were measured (available in43, where the final cleaned dataset is found in the ‘Traits’ folder). Specifically, the site-level leaf sampling outside the experimental plots resulted in 4,894 leaves and 193 taxa, with a variable number of observations per site (Lowland = 1,387; Middle = 1,436; Alpine = 1,065; High alpine = 1,006). In addition, the within-treatment level sampling resulted in 1,777 leaves; with 14–404 individuals per species.
Because many specimens had very small leaves, it was necessary to merge some individuals to obtain enough material for the chemical traits. A subset of 576 such combined leaf samples (265 from the site-level samples of sites along the gradient; 311 from the within-treatment level sampling) were thus used to assess leaf chemical or nutrient (Carbon [C], Nitrogen [N], Phosphorus, C:N ratio) and isotope (C, N) traits (available in43, where the final cleaned dataset is found in the ‘Traits’ folder).
Because of leaf merging and hence differences in resolution, the leaf and chemical traits are provided in two data sets. Unweighted trait distributions of all data per site are provided in Fig. 4. The trait data covered between 57% and 85% of the species present in the plant community, based on sum of covers from the control plots, and between 97% and 100% of the biomass at each site comprised of genera with trait data (calculations based on datasets i and iii).
Datasets (v–vii): Climate
Climate station data was collected, but is of variable quality due to setup and operational errors. To mitigate this, we have extracted partial data for parameters and periods with more reliable data, and augmented these with short-term data from iButton and TomsT loggers. We thus report on climate station air temperature at 2 m at each site along the gradient between December 2012 and October 2016 (available in43, where the final cleaned dataset is found in the ‘Climate’ folder). This dataset contains 1,176,156 observations. Average annual temperature in the four sites was 4.73 °C in the Lowland, 3.92 °C in the Middle, 2.05 °C in the Alpine and 1.5 °C in the High alpine site (Dataset v, Fig. 5, Table 7). From the lowest to the highest elevation sites, the mean growing season temperature (June-August) decreased from 12.0 and 6.7 °C, growing degree days above 5 °C decreased from 112–181, and number of frost days (days below 0 °C) increased from 90 to 165 (see Table 1). Unfortunately, the quality of the climate data does not allow us to calculate snow duration (specifically, soil and ground layer temperature loggers were installed for this purpose, but the data are compromised). Snow depth was not measured. We do note, however, that snow cover is not constant, but scattered and highly variable during the winter season at all four sites (Y. Yang, personal observation).
The iButton dataset (available in43, where the final cleaned dataset is found in the ‘Climate’ folder) contains temperature data through the growing season 2017 (Dataset vi, Tables 2 and 8). This dataset contains 444,035 observations with ca. 140,000 observations for each depth. The warmed plots in the transplants and OTC were on average 0.48–1.74 °C and 0.7–1.58 °C warmer than the control plots (dataset vi, Fig. 5, Table 8).
From September 2019, air and soil temperature and soil moisture was measured by TomsT loggers in three locations inside and outside the OTC in the High alpine site (available in43, where the final cleaned dataset is found in the ‘Climate’ folder). The OTCs had on average higher temperatures compared to the control plots: the difference for air, ground and soil temperature was (mean ± SE) 1.6 ± 0.06 °C, 1.4 ± 0.05 °C and 0.9 ± 0.03 °C, respectively (Dataset vii, Fig. 5, Table 9).
During the 5-year data collection period a number of different people were involved, which increases the risk of observation errors in the data set. In particular, species can be misidentified (i.e., sterile graminoids) in the community or traits data or might be overlooked in one of the community censuses. These errors will result in pseudo-turnover in the plant community data and may affect trait distributions. To detect and correct such errors in the community data, we compared each recorded species in each subplot over the 5-year time-series. We used the subplot data to assign unidentified or missing species to an existing identified species in that subplot if it was likely (e.g., from field notes, description or a given lay or generic name) that this could be the correct ID, and if there was a record of that species in the subplot in the previous and following year. Further, we re-estimated species covers in cases where cover was either missing or clearly too low or high to be realistic when comparing with the total sum of covers and covers of the same species in adjacent years in the time-series. We replaced such erroneous values with the mean cover from the previous and following year. We did such re-estimations for a total of 48 occasions (c. 1% of the whole 5-year dataset). The data-checking code and outcomes for these various procedures is documented in44.
Both trait and community taxonomy was checked and corrected against TNRS37. A full species list of all identified species across datasets is also available in the OSF repository (see below). In addition, there are 10 unidentified taxa in the plant community (dataset i), 29 in the biomass (dataset iii) and 16 in the traits (dataset iv). Note that unknown taxa were not harmonized between the datasets, so that Genus sp1 in the trait dataset is not necessarily the same as Genus sp1 in the biomass dataset.
Experimental treatment effects and side-effects
Generally, OTCs increase mean daily air temperature by ca. 1.5 °C24. Unfortunately, due to extensive climate logger failure in our experiment we do not have continuous time-series of measurements from the OTCs, but we do have measurements from shorter periods in the growing season in 2017 and in the fall of 2019 indicating that our OTC have similar warming effects to those reported elsewhere (Fig. 5, Datasets vi, vii).
For the transplant experiment, one plot at each block per site was transplanted to the corresponding block of the site at the lower elevation and one plot at the higher elevation, representing a c. 1.75 °C warming or cooling of the summer temperature (i.e., reflecting the temperature difference between each site, dataset v). The extreme transplants were subjected to a 5.3 °C warming or cooling, respectively (i.e., the temperature difference between the High alpine and Lowland sites, dataset v).
Analysis of the local transplant and the untouched control plots across all years show that there were no differences between them in any of the measured predictor or response variables, and thus there were no unwanted biotic or abiotic effects of the turf cutting and transplanting, as also shown in a similar transplant experiment elsewhere using the same approach26.
Trait data validation
Trait data were thoroughly checked and validated as follows. First, we checked and corrected missing or erroneous sample identifications in one or more of the measurements against field notes and notes on the leaf envelopes. Second, unrealistically high or low values of one or more trait values were checked and corrected against the lab and field notes for typing errors. Any remaining samples with apparent measurement errors that resulted in unrealistic trait values were removed. This was done for leaves with clearly erroneous leaf area values (empty scans, double scans, blank areas within the leaf perimeter, dirt or other non-leaf objects on scans, wrong match between scan and leaf ID, etc.), leaf dry matter values higher than 1 g/g, leaves with specific leaf area values less than 5 cm2/g or greater than 500 cm2/g and leaf nitrogen values higher than 6.4% (see the code and associated readme file44 for details). The nitrogen cut-off values was chosen based on global leaf nitrogen values in the Botanical Information and Ecology Network datasets30 for the genera in our study. We further plotted the data (e.g., wet mass vs dry mass) and checked for outliers. Some leaves were very small, and dry weights may be approaching the lower limits of weight sensitivity of the balances. In these cases, the data were removed. The data checking code and outcomes for these various procuedures is available and documented in the code and associated readme file44.
To properly use these data, be aware that (a) in the community data, graminoids are generally not identified to the species level, due to difficulty in identifying sterile graminoids in this regional flora, etc. (see above). The graminoids thus have lower taxonomic resolution than the forbs. (b) Also in the community data, cover values may be prone to observer error due to different observers between years. (c) In the traits data, we have followed what we consider best practice for data quality, and filtered out what we consider unreliable data, e.g., dry mass for very small leaves approaching the limits for balance accuracy, leaf area in the case of clearly erroneous scans, clearly unrealistic measurements (see above). The procedures and consequences are detailed in the code and associated readme file. Users who might prefer a stricter or more inclusive data handling strategy should check the flags in the raw data sets and adjust the data cleaning accordingly. (f) Note that unidentified taxa are not harmonized across datasets. See the code and associated readme file44 for our suggested data cleaning and checking procedures that result in producing what we consider the clean and ‘best practice’ final datasets (found in separate folders per dataset in43), and the various ‘Flag’ and ‘Comment’ columns in the different dataset tables (Tables 3, 4 and 6) that indicate additional specific data points or leaves (i.e., data rows) that could be removed to create even more robust datasets.
The code used for checking, cleaning and analysing the data is available in the open GitHuB repository “https://github.com/Plant-Functional-Trait-Course/PFTC_1_2_China”, of which a versioned copy is available at Zenodo44. There is also a link to the code from the published dataset43.
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This research was conducted at the Alpine Ecosystem Observation and Experiment Station of Mt. Gongga, Chinese Academy of Sciences (CAS), which provided accommodation and lab space. Funding was provided by the collaborative project between NSFC-RCN funded by the National Natural Science Foundation of China (Grant no. 41861134039), and the Norwegian Center for International Cooperation in Education (SIU) (Grant No. 2013/10074 and HNP2015/10037). We thank Vanessa Buzzard for help with planning and conducting the trait campaign and analyses and comments on earlier versions of this paper, Yang Yang for assistance with community composition data, Anjeanette McKay for assistance with stoichiometric and isotope analysis, Kine Blom, Bárbara Javiera Seaman Espinosa, Frida Knoop, Christine Pötsch, and Fei Ran for assistance in the field and lab, and internship students at the University of Arizona for assistance with leaf chemical trait analyses.
The authors declare no competing interests.
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Vandvik, V., Halbritter, A.H., Yang, Y. et al. Plant traits and vegetation data from climate warming experiments along an 1100 m elevation gradient in Gongga Mountains, China. Sci Data 7, 189 (2020). https://doi.org/10.1038/s41597-020-0529-0