Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Guidance for quantitative confocal microscopy

This poster guides researchers through all aspects of acquiring quantitative confocal microscopy images. It provides recommendations from sample preparation to data acquisition and discusses common experimental and instrumentation pitfalls.

This poster is freely available online thanks to support from Carl Zeiss Microscopy GmbH.

The poster content is peer reviewed, editorially independent and the sole responsibility of Nature America, Inc., part of Springer Nature.

Change history

  • 20 April 2020

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Author information

Authors and Affiliations


Corresponding author

Correspondence to James Jonkman.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Jonkman, J., Brown, C.M., Wright, G.D. et al. Guidance for quantitative confocal microscopy. Nat Protoc (2020).

Download citation

  • Published:

  • DOI:


Quick links