Classic approaches to mapping the developmental history of cells in vivo have relied on techniques that require complex interventions and often capture only a single trajectory or moment in time. We have previously described a developmental barcoding system to address these issues using synthetically induced mutations to record information about each cell’s lineage in its genome. This system uses MARC1 mouse lines, which have multiple homing guide RNAs that each generate hundreds of mutant alleles and combine to produce an exponential diversity of barcodes. Here, we detail two MARC1 lines that are available from a public repository. We describe strategies for using MARC1 mice and experimental design considerations. We provide a protocol for barcode retrieval and sequencing as well as the analysis of the sequencing data. This protocol generates barcodes based on synthetically induced mutations in mice to enable lineage analysis.
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All sequencing data are available in the Sequence Read Archive under accession SRP155997; the parts of this dataset that correspond to the PB7 line were previously published7. Figs. 3–5 contain associated raw data for both PB3 and PB7 lines which are available in Supplementary Tables 1–4 and SRP155997.
All code is available from our GitHub repository: https://github.com/Kalhor-Lab/MARC1-Pipeline. Code in this protocol has been peer reviewed.
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The authors acknowledge J. Dapello for comments on the manuscript and L. Mejia for assistance in preparing sequencing libraries. The authors also acknowledge the team at MMRRC and UC-Davis Mouse Biology Program for making it possible to distribute MARC1 lines. This work was supported by Johns Hopkins Institutional Funds, grants from the Simons Foundation (SFARI 606178, R.K.), the NIH (MH103910, G.M.C. and R01GM123313, P.M.) and the Intelligence Advanced Research Projects Activity (IARPA) via the Department of Interior/Interior Business Center (DoI/IBC) contract number D16PC00008.
G.M.C.’s competing financial interests can be found at v.ht/PHNc. The remaining authors declare no competing financial interest.
Peer review information Nature Protocols thanks the anonymous reviewers for their contribution to the peer review of this work.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Key reference using this protocol
Kalhor, R. et al. Science 361, eaat9804 (2018): https://doi.org/10.1126/science.aat9804
Supplementary Figs. 1–4, Supplementary Table 2 and legends for Supplementary Tables 1 and 3–8.
List of all hgRNAs in the MARC1 founder males. For each hgRNA, its assigned number, ID sequence, spacer sequence, TSS to PAM length, spacer length, observed inheritance probability, chromosomal location, and confidence level of the chromosomal location are given. A “+” sign in the location column indicates that the hgRNA is transcribed in the same direction as the positive strand of the mm10 reference sequence.
Average observed mutation level of each hgRNA during different stages of development. Values indicate percent mutated while “NA” indicates values that were not measured. Functional category based on this mutation level is also shown. Barcodes with <2% maximum mutation rate in all samples were assigned as “inactive”, those with 2–50% maximum mutation rate in all samples were assigned as “slow”, those with >90% average mutation frequency at any point before E9 were assigned as “fast”, and the rest were assigned as “mid.”
For each mutant spacer allele of each hgRNA, its sequence, occurrence probability (fraction of mice with that hgRNA which contained the allele), occurrence (number of mice out of total with that hgRNA which contained the allele), and median abundance (median of its abundance fraction in all mice in which it was observed) are shown.
Example genotyping output of the analysis pipeline, corresponding to a mutated sample. A [sampleName]_genotypes.txt file is produced for each sample. It provides each identifier (# and ID) and the most commonly observed associated spacer sequence (SP), forming an identifier–spacer pair; the absolute count of observations of this pair (count); the percent of this pair relative to all observed pairs in a sample; and the percent of this pair relative to all pairs with that identifier.
Example filtered pairs output of the analysis pipeline on a mutated sample. A [sample]_filteredpairs.txt file, produced for each sample, contains information on mutation levels and all observed pairs. This table contains the observed identifier with a corresponding observed spacer, the total observation count for that pair, the percent of that pair within all observations of that identifier, and the percent of that pair within all sample observations.
Suggested primer sets for specific amplification of each hgRNA in non-barcoded mice. Each pair is expected to produce an amplicon of 141–155 bp in length. Using each primer pair in a standard PCR on genomic DNA of MARC1 mice can establish the presence of the targeted hgRNA if an amplicon of the correct size appears. Absence of the correct amplicon in the PCR product would indicate absence of the hgRNA. These primer sets are not experimentally validated and might fail to amplify mutated hgRNA sequences, as they depend on the spacer region to bind.
Table of observed mutant allele frequencies in each sample in Fig. 5. Rows correspond to samples and columns to each of the mutant spacer alleles that were observed. Alleles with <0.25% abundance in each sample are not included. parSP indicates spacer sequence observed in founder, mutSP indicates mutant spacer sequences observed in samples under analysis.
Data analysis pipeline.
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Leeper, K., Kalhor, K., Vernet, A. et al. Lineage barcoding in mice with homing CRISPR. Nat Protoc 16, 2088–2108 (2021). https://doi.org/10.1038/s41596-020-00485-y
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