Fig. 4: Differential RNA editing using REDItools and REDIportal. | Nature Protocols

Fig. 4: Differential RNA editing using REDItools and REDIportal.

From: Investigating RNA editing in deep transcriptome datasets with REDItools and REDIportal

Fig. 4

REDItools and REDIportal can be used in combination to identify differential RNA editing at known sites, i.e., genomic positions in which RNA editing levels are statistically different between two or more conditions. The procedure requires aligned RNAseq data and begins with the selection of known events to explore from REDIportal. Then, REDItools are launched on each RNAseq sample, providing a series of output tables (Steps 1–4, Procedure 2). All REDItools tables are parsed and compared according to metadata, returning a final list of known sites with indications of differential editing (Steps 5 and 6, Procedure 2).

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