Supplementary Figure 1: Additional illustrative output plots generated by the recoRdseq R package, with samples color-coded by experimental group specified in the design matrix. | Nature Protocols

Supplementary Figure 1: Additional illustrative output plots generated by the recoRdseq R package, with samples color-coded by experimental group specified in the design matrix.

From: Recording transcriptional histories using Record-seq

Supplementary Figure 1

a. Mean untransformed genome-aligning spacer counts by group (error bars represent standard error of the mean). b. PCA plots for rlog transformed gene-aligning spacer counts for the top 50 variable genes with clusters defined by k-means clustering (k=2). c. Venn diagram showing overlap in significant DE genes (padj < 0.05) detected by the three DE tools (DESeq2, edgeR and baySeq). d. Heatmap showing unsupervised hierarchical clustering of samples based on rlog transformed genome-aligning spacer counts for high-confidence DE genes detected by the three DE tools. For all panels, n=6 independent biological replicates.

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