Supplementary Fig. 4: Result of autofocus simulation for four algorithms. | Nature Protocols

Supplementary Fig. 4: Result of autofocus simulation for four algorithms.

From: Advanced CUBIC tissue clearing for whole-organ cell profiling

Supplementary Fig. 4

Result of autofocus simulation. 400,000 images data sets (400 of different light-sheet positions with 0.35 μm span) which was acquired by our LSFM at the 5 μm step size covering 5000 μm depth were used. MOVIE used parameters optimized in Supplementary Fig. 2a. “Without autofocus” means the constant light-sheet position throughout. “Conventional Autofocus” was stop and exposure autofocus mode updated every 1000 μm. An ideal focus position was determined by the light-sheet position with the highest DCTS score of 400 images at each z position. rDCTS (relative DCTS) was calculated with division by DCTS of ideal focus positions. Scale bar, 10 μm.

Back to article page