Supplementary Fig. 6: Assembly of microrespirator. | Nature Protocols

Supplementary Fig. 6: Assembly of microrespirator.

From: Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips

Supplementary Fig. 6

a. i) Attach corner brackets to aluminum extrusions uses t-slot nuts and bolts, ii) attach endMotor piece to corner brackets using nuts and bolts, iii) attach motors to endMotor piece using M3 screws, iv) attach shaft couplers to motor shaft by gently tightening set screw, v) attach corner brackets to aluminum extrusions as above, vi) attach endThrustBearing piece to corner rackets as above. b. i) Attach corner brackets to aluminum extrusions as above, ii) hold the syringeBase piece such that the holes for the syringes are vertically oriented, iii) place the syringes into these holes, such that they hang down by gravity, with the shoulder of the syringe resting on the syringeBase piece, iv) align the syringeBaseEtch piece and secure the assembly by inserting the central screws gently tightening their nuts. Secure this subassembly to corner brackets as above. c. i) Insert the GasketMaterialCircular pieces into the holes etched into the pistonEnd piece, ii) align the pistonHole piece with the pistonEnd-Hole piece and hold them together such that the circular holes oriented vertically, as above, and drop the pistons into the holes, iii) carefully align this subassembly with the pistonEnd piece containing the GasketMaterialCircular pieces, iv) Insert the thrust bearings and gently secure them with nuts and bolts. Attach the lead screw nuts gently, using nuts and bolts, v) the syringe pistons can now be aligned and inserted into the syringes. d. i) Insert the linear motion shafts through the syringeBase and syringeBaseEtch pieces, very loosely placing a collar clamps onto the shafts before they are inserted into the thrust bearings, through the endThrustBearing piece, and finally secured to the endMotor piece using screws, ii) terminal collar clamps can now be slid onto the ends and all collar clamps gently tightened, iii) threaded rods can be inserted, with a small collar clamp and thrust bearing put on each, before they pass through the pistonEnd piece. At this point, each threaded rod should have another thrust bearing and small collar clamp slid on before the threaded rod is secured by the shaft coupler, iv) the limit switch holder and limit switch can be attached, v) all pieces can be gently tightened, vi) the shelfSliders can be attached using t-slot nuts and bolts, vii) the fans should be oriented such that the air flow is directed at the motors and can be attached to the endMotor piece using zip ties. e. Wire the electronic components as shown in this wiring diagram. Specific communication ports on the Arduino should agree with the control software.

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