Transgenesis in Hydra to characterize gene function and visualize cell behavior

Abstract

The freshwater polyp Hydra is a cnidarian used as a model organism in a number of fields, including the study of the origin and evolution of developmental mechanisms, aging, symbiosis and host–microbe interactions. Here, we describe a procedure for the establishment of stable transgenic Hydra lines by embryo microinjection. The three-stage protocol comprises (i) the design and preparation of a transgenic construct, (ii) the microinjection of the vector into early embryos of Hydra vulgaris, and (iii) the selection and enrichment of mosaic animals in order to develop uniformly transgenic clonal lines. The preparation of a transgenic construct requires ~2 weeks, and transgenic lines can be obtained within 3 months. The method allows constitutive or inducible gain- and loss-of-function approaches, as well as in vivo tracing of individual cells. Hydra polyps carrying transgenic cells reveal functional properties of the ancestral circuitry controlling animal development.

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Fig. 1: Overview of the Hydra transgenesis technology.
Fig. 2: Embryo microinjection allows genetic manipulation of all cell types in the transgenic Hydra lines.
Fig. 3: The diversity of available constructs ensures high versatility of the transgenic Hydra technology.
Fig. 4: High-density culture and regular intensive feeding before sex induction allow maximization of the yield of fertilized eggs from a mass culture of Hydra.
Fig. 5: Two clonal lines should be raised from each mosaic founder polyp.
Fig. 6: Evidence for germline transmission of a transgene.

Data availability

All data generated or analyzed during the current study are included in this paper. The raw data used in the example results are available from the corresponding authors upon reasonable request.

References

  1. 1.

    Galliot, B. Hydra, a fruitful model system for 270 years. Int. J. Dev. Biol. 56, 411–423 (2012).

    CAS  Article  Google Scholar 

  2. 2.

    Siebert, S., Anton-Erxleben, F. & Bosch, T. C. G. Cell type complexity in the basal metazoan Hydra is maintained by both stem cell based mechanisms and transdifferentiation. Dev. Biol. 313, 13–24 (2008).

    CAS  Article  Google Scholar 

  3. 3.

    Gee, L. et al. β-catenin plays a central role in setting up the head organizer in Hydra. Dev. Biol. 340, 116–124 (2010).

    CAS  Article  Google Scholar 

  4. 4.

    Hemmrich, G. et al. Molecular signatures of the three stem cell lineages in Hydra and the emergence of stem cell function at the base of multicellularity. Mol. Biol. Evol. 29, 3267–3280 (2012).

    CAS  Article  Google Scholar 

  5. 5.

    Boehm, A.-M. et al. FoxO is a critical regulator of stem cell maintenance in immortal Hydra. Proc. Natl. Acad. Sci. USA 109, 19697–19702 (2012).

    CAS  Article  Google Scholar 

  6. 6.

    Domazet-Lošo, T. et al. Naturally occurring tumours in the basal metazoan Hydra. Nat. Commun. 5, 4222 (2014).

    Article  Google Scholar 

  7. 7.

    Klimovich, A. et al. Non-senescent Hydra tolerates severe disturbances in the nuclear lamina. Aging 10, 951–972 (2018).

    Article  Google Scholar 

  8. 8.

    Anton-Erxleben, F., Thomas, A., Wittlieb, J., Fraune, S. & Bosch, T. C. G. Plasticity of epithelial cell shape in response to upstream signals: a whole-organism study using transgenic Hydra. Zoology 112, 185–194 (2009).

    Article  Google Scholar 

  9. 9.

    Nishimiya-Fujisawa, C. & Kobayashi, S. Roles of germline stem cells and somatic multipotent stem cells in Hydra sexual reproduction. in Reproductive and Developmental Strategies: The Continuity of Life (eds Kobayashi, K., Kitano, T., Iwao, Y. & Kondo, M.) 123–155 (Springer Japan, 2018).

  10. 10.

    Boehm, A.-M. & Bosch, T. C. G. Migration of multipotent interstitial stem cells in Hydra. Zoology 115, 275–282 (2012).

    Article  Google Scholar 

  11. 11.

    Mortzfeld, B. M. & Bosch, T. C. G. Eco-aging: stem cells and microbes are controlled by aging antagonist FoxO. Curr. Opin. Microbiol. 38, 181–187 (2017).

    CAS  Article  Google Scholar 

  12. 12.

    Mortzfeld, B. M., Taubenheim, J., Fraune, S., Klimovich, A. V. & Bosch, T. C. G. Stem cell transcription factor FoxO controls microbiome resilience in Hydra. Front. Microbiol. 9, 629 (2018).

    Article  Google Scholar 

  13. 13.

    Koizumi, O. Origin and evolution of the nervous system considered from the diffuse nervous system of cnidarians. in The Cnidaria, Past, Present and Future (eds Goffredo, S. & Dubinsky, Z.) 73–91 (Springer, Basel, Switzerland, 2016).

  14. 14.

    Bosch, T. C. G. et al. Back to the basics: cnidarians start to fire. Trends Neurosci. 40, 92–105 (2017).

    CAS  Article  Google Scholar 

  15. 15.

    Klimovich, A. V & Bosch, T. C. G. Rethinking the role of the nervous system: lessons from the Hydra holobiont. BioEssays e1800060 (2018).

  16. 16.

    Dupre, C. & Yuste, R. Non-overlapping neural networks in Hydra vulgaris. Curr. Biol. 27, 1085–1097 (2017).

    CAS  Article  Google Scholar 

  17. 17.

    Rentzsch, F., Juliano, C. & Galliot, B. Modern genomic tools reveal the structural and cellular diversity of cnidarian nervous systems. Curr. Opin. Neurobiol. 56, 87–96 (2019).

    CAS  Article  Google Scholar 

  18. 18.

    Bosch, T. C. G. Rethinking the role of immunity: lessons from Hydra. Trends Immunol. 35, 495–502 (2014).

    CAS  Article  Google Scholar 

  19. 19.

    Augustin, R., Fraune, S., Franzenburg, S. & Bosch, T. C. G. Where simplicity meets complexity: hydra, a model for host–microbe interactions. in Recent Advances on Model Hosts (eds Mylonakis, E., Ausubel, F. M., Gilmore, M. & Casadevall, A.) 71–81 (Springer, Basel, Switzerland, 2012).

  20. 20.

    Franzenburg, S. et al. MyD88-deficient Hydra reveal an ancient function of TLR signaling in sensing bacterial colonizers. Proc. Natl. Acad. Sci. USA 109, 19374–19379 (2012).

    CAS  Article  Google Scholar 

  21. 21.

    Franzenburg, S. et al. Distinct antimicrobial peptide expression determines host species-specific bacterial associations. Proc. Natl. Acad. Sci. USA 110, E3730–E3738 (2013).

    CAS  Article  Google Scholar 

  22. 22.

    Augustin, R. et al. A secreted antibacterial neuropeptide shapes the microbiome of Hydra. Nat. Commun. 8, 698 (2017).

    Article  Google Scholar 

  23. 23.

    Chapman, J. A. et al. The dynamic genome of Hydra. Nature 464, 592–596 (2010).

    CAS  Article  Google Scholar 

  24. 24.

    Hemmrich, G. & Bosch, T. C. G. Compagen, a comparative genomics platform for early branching metazoan animals, reveals early origins of genes regulating stem-cell differentiation. Bioessays 30, 1010–1018 (2008).

    CAS  Article  Google Scholar 

  25. 25.

    Wittlieb, J., Khalturin, K., Lohmann, J. U., Anton-Erxleben, F. & Bosch, T. C. G. Transgenic Hydra allow in vivo tracking of individual stem cells during morphogenesis. Proc. Natl. Acad. Sci. USA 103, 6208–6211 (2006).

    CAS  Article  Google Scholar 

  26. 26.

    Steele, R. E. Trembley’s polyps go transgenic. Proc. Natl. Acad. Sci. USA 103, 6415–6416 (2006).

    CAS  Article  Google Scholar 

  27. 27.

    Wein, T. et al. Carrying capacity and colonization dynamics of Curvibacter in the Hydra host habitat. Front. Microbiol. 9, 443 (2018).

    Article  Google Scholar 

  28. 28.

    Murillo-Rincon, A. P. et al. Spontaneous body contractions are modulated by the microbiome of Hydra. Sci. Rep. 7, 15937 (2017).

    Article  Google Scholar 

  29. 29.

    Juliano, C. E., Lin, H. & Steele, R. E. Generation of transgenic Hydra by embryo microinjection. J. Vis. Exp. 2014, 51888 (2014).

    Google Scholar 

  30. 30.

    Böttger, A. et al. GFP expression in Hydra: lessons from the particle gun. Dev. Genes Evol. 212, 302–305 (2002).

    Article  Google Scholar 

  31. 31.

    Dana, C. E., Glauber, K. M., Chan, T. A., Bridge, D. M. & Steele, R. E. Incorporation of a horizontally transferred gene into an operon during cnidarian evolution. PLoS ONE 7, e31643 (2012).

    CAS  Article  Google Scholar 

  32. 32.

    Milde, S. et al. Characterization of taxonomically restricted genes in a phylum-restricted cell type. Genome Biol. 10, R8 (2009).

    Article  Google Scholar 

  33. 33.

    Khalturin, K. et al. A novel gene family controls species-specific morphological traits in Hydra. PLoS Biol. 6, e278 (2008).

    Article  Google Scholar 

  34. 34.

    Khalturin, K. et al. Transgenic stem cells in Hydra reveal an early evolutionary origin for key elements controlling self-renewal and differentiation. Dev. Biol. 309, 32–44 (2007).

    CAS  Article  Google Scholar 

  35. 35.

    Juliano, C. E. et al. PIWI proteins and PIWI-interacting RNAs function in Hydra somatic stem cells. Proc. Natl. Acad. Sci. USA 111, 337–342 (2014).

    CAS  Article  Google Scholar 

  36. 36.

    Iachetta, R. et al. Real time dynamics of β-catenin expression during Hydra development, regeneration and Wnt signalling activation. Int. J. Dev. Biol. 62, 311–318 (2018).

    CAS  Article  Google Scholar 

  37. 37.

    Nakamura, Y., Tsiairis, C. D., Özbek, S. & Holstein, T. W. Autoregulatory and repressive inputs localize Hydra Wnt3 to the head organizer. Proc. Natl. Acad. Sci. USA 108, 9137–9142 (2011).

    CAS  Article  Google Scholar 

  38. 38.

    Noro, Y. et al. Regionalized nervous system in Hydra and the mechanism of its development. Gene Expr. Patterns 31, 42–59 (2019).

    CAS  Article  Google Scholar 

  39. 39.

    Lippincott-Schwartz, J. & Patterson, G. H. Development and use of fluorescent protein markers in living cells. Science 300, 87–91 (2003).

    CAS  Article  Google Scholar 

  40. 40.

    Li, X. et al. Generation of destabilized green fluorescent protein as a transcription reporter. J. Biol. Chem. 273, 34970–34975 (1998).

    CAS  Article  Google Scholar 

  41. 41.

    Bosch, T., Anton-Erxleben, F., Hemmrich, G. & Khalturin, K. The Hydra polyp: nothing but an active stem cell community. Dev. Growth Differ. 52, 15–25 (2010).

    CAS  Article  Google Scholar 

  42. 42.

    Buzgariu, W., Crescenzi, M. & Galliot, B. Robust G2 pausing of adult stem cells in Hydra. Differentiation 87, 83–99 (2014).

    CAS  Article  Google Scholar 

  43. 43.

    Wenger, Y., Buzgariu, W. & Galliot, B. Loss of neurogenesis in Hydra leads to compensatory regulation of neurogenic and neurotransmission genes in epithelial cells. Philos. Trans. R. Soc. B Biol. Sci. 371, 20150040 (2016).

  44. 44.

    Siebert, S. et al. Stem cell differentiation trajectories in Hydra resolved at single-cell resolution. Preprint at bioRxiv, https://doi.org/10.1101/460154 (2018).

  45. 45.

    Lenhoff, M. & Dubois, R. A. Y. Mass culture of Hydra: an improved method and its application to other aquatic invertebrates. Lab. Anim. 4, 139–154 (1970).

    CAS  Article  Google Scholar 

  46. 46.

    Lenhoff, H. M. Culturing large numbers of Hydra in Hydra: Research Methods 53–62 (Springer, New York, 1983).

  47. 47.

    Bossert, P. & Galliot, B. How to use Hydra as a model system to teach biology in the classroom. Int. J. Dev. Biol. 56, 637–652 (2012).

    Article  Google Scholar 

  48. 48.

    Johnson, E. M. & Gabel, B. E. G. An artificial ‘embryo’ for detection of abnormal developmental biology. Toxicol. Sci. 3, 243–249 (1983).

    CAS  Article  Google Scholar 

  49. 49.

    Johnson, E. M. & Gabel, B. E. G. Application of the Hydra assay for rapid detection of developmental hazards. J. Am. Coll. Toxicol. 1, 57–71 (1982).

    CAS  Article  Google Scholar 

  50. 50.

    Renfer, E. & Technau, U. Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis. Nat. Protoc. 12, 1844 (2017).

    CAS  Article  Google Scholar 

  51. 51.

    Bosch, T. C. & David, C. N. Male and female stem cells and sex reversal in Hydra polyps. Proc. Natl. Acad. Sci. USA 83, 9478–9482 (1986).

    CAS  Article  Google Scholar 

  52. 52.

    Nishimiya-Fujisawa, C. & Kobayashi, S. Germline stem cells and sex determination in Hydra. Int. J. Dev. Biol. 52, 499–508 (2012).

    Article  Google Scholar 

  53. 53.

    Glauber, K. M. et al. A small molecule screen identifies a novel compound that induces a homeotic transformation in Hydra. Development 140, 4788–4796 (2013).

    CAS  Article  Google Scholar 

  54. 54.

    Bosch, T. C. G. & David, C. N. Stem cells of Hydra magnipapillata can differentiate into somatic cells and germ line cells. Dev. Biol. 121, 182–191 (1987).

    Article  Google Scholar 

  55. 55.

    Nishimiya-Fujisawa, C. & Sugiyama, T. Genetic analysis of developmental mechanisms in Hydra. XXII. Two types of female germ stem cells are present in a male strain of Hydra magnipapillata. Dev. Biol. 172, 324–336 (1995).

    CAS  Article  Google Scholar 

  56. 56.

    Magie, C. R., Daly, M. & Martindale, M. Q. Gastrulation in the cnidarian Nematostella vectensis occurs via invagination not ingression. Dev. Biol. 305, 483–497 (2007).

    CAS  Article  Google Scholar 

  57. 57.

    Rentzsch, F., Fritzenwanker, J. H., Scholz, C. B. & Technau, U. FGF signalling controls formation of the apical sensory organ in the cnidarian Nematostella vectensis. Development 135, 1761–1769 (2008).

    CAS  Article  Google Scholar 

  58. 58.

    Layden, M. J., Röttinger, E., Wolenski, F. S., Gilmore, T. D. & Martindale, M. Q. Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis. Nat. Protoc. 8, 924–934 (2013).

    Article  Google Scholar 

  59. 59.

    Lommel, M. et al. Genetic knockdown and knockout approaches in Hydra. Preprint at https://www.biorxiv.org/content/10.1101/460154v1 (2017).

  60. 60.

    Bosch, T. C. G., Augustin, R., Gellner, K., Khalturin, K. & Lohmann, J. U. In vivo electroporation for genetic manipulations of whole Hydra polyps. Differ. Orig. Artic. 70, 140–147 (2002).

    CAS  Google Scholar 

  61. 61.

    Miljkovic, M., Mazet, F. & Galliot, B. Cnidarian and bilaterian promoters can direct GFP expression in transfected Hydra. Dev. Biol. 246, 377–390 (2002).

    CAS  Article  Google Scholar 

  62. 62.

    Chera, S. et al. Silencing of the Hydra serine protease inhibitor Kazal1 gene mimics the human SPINK1 pancreatic phenotype. J. Cell Sci. 119, 846–857 (2006).

    CAS  Article  Google Scholar 

  63. 63.

    Galliot, B., Miljkovic-Licina, M., Ghila, L. & Chera, S. RNAi gene silencing affects cell and developmental plasticity in Hydra. C. R. Biol. 330, 491–497 (2007).

    CAS  Article  Google Scholar 

  64. 64.

    Ambrosone, A., Marchesano, V., Tino, A., Hobmayer, B. & Tortiglione, C. Hymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris. PLoS ONE 7, e30660 (2012).

    CAS  Article  Google Scholar 

  65. 65.

    Schwentner, M. & Bosch, T. C. G. Revisiting the age, evolutionary history and species level diversity of the genus Hydra (Cnidaria: Hydrozoa). Mol. Phylogenet. Evol. 91, 41–55 (2015).

    Article  Google Scholar 

  66. 66.

    Bosch, T. C. G. et al. How do environmental factors influence life cycles and development? An experimental framework for early-diverging metazoans. BioEssays 36, 1185–1194 (2014).

    Article  Google Scholar 

  67. 67.

    Hamada, M. et al. Metabolic co-dependence drives the evolutionarily ancient HydraChlorella symbiosis. Elife 7, e35122 (2018).

    Article  Google Scholar 

  68. 68.

    Revilla-i-Domingo, R., Schmidt, C., Zifko, C. & Raible, F. Establishment of transgenesis in the demosponge Suberites domuncula. Genetics 210, 435–443 (2018).

    CAS  Article  Google Scholar 

  69. 69.

    Hemmrich, G., Anokhin, B., Zacharias, H. & Bosch, T. C. G. Molecular phylogenetics in Hydra, a classical model in evolutionary developmental biology. Mol. Phylogenet. Evol. 44, 281–290 (2007).

    CAS  Article  Google Scholar 

  70. 70.

    Martínez, D. E. et al. Phylogeny and biogeography of Hydra (Cnidaria: Hydridae) using mitochondrial and nuclear DNA sequences. Mol. Phylogenet. Evol. 57, 403–410 (2010).

    Article  Google Scholar 

  71. 71.

    Nakamura, Y., Gojobori, T. & Ikemura, T. Codon usage tabulated from international DNA sequence databases: status for the year 2000. Nucleic Acids Res. 28, 292 (2000).

    CAS  Article  Google Scholar 

  72. 72.

    Oesterle, A. Pipette Cookbook 2015: P-97 & P-1000 Micropipette Pullers (Sutter Instrument, Novato, CA, 2015).

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Acknowledgements

We are grateful to F. Anton-Erxleben for assistance with confocal microscopy and to J. Lohmann and K. Khalturin for contributions to the development of the genetic constructs. This work was supported in part by the Deutsche Forschungsgemeinschaft (DFG) (grant CRC 190, ‘Mechanisms and factors of gene activation’; grant nos. 846/6-1-4, 848/13-1-4, and 848/15-1; grant CRC 1182, ‘Origin and function of Metaorganisms’; and grants from the DFG Clusters of Excellence ‘Inflammation at Interfaces’ and ‘The Future Ocean’). A.K. was supported by a fellowship from the Alexander von Humboldt Foundation. T.C.G.B. acknowledges support from the Canadian Institute for Advanced Research (CIFAR).

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A.K. and T.C.G.B. conceived the paper. J.W. and A.K. generated the data and took photos. A.K., J.W. and T.C.G.B. wrote the manuscript.

Corresponding authors

Correspondence to Alexander Klimovich or Thomas C. G. Bosch.

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The authors declare no competing interests.

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Journal peer review information: Nature Protocols thanks Brigitte Galliot, Rob Steele and other anonymous reviewer(s) for their contribution to the peer review of this work.

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Key references using this protocol

Wittlieb, J., Khalturin, K., Lohmann, J. U., Anton-Erxleben, F. & Bosch, T. C. G. Proc. Natl. Acad. Sci. USA 103, 6208–6211 (2006): https://doi.org/10.1073/pnas.0510163103

Siebert, S., Anton-Erxleben, F. & Bosch, T. C. G. Dev. Biol. 313, 13–24 (2008): https://doi.org/10.1016/j.ydbio.2007.09.007

Klimovich, A. et al. Aging 10, 951–972 (2018): https://doi.org/10.18632/aging.101440

Integrated supplementary information

Supplementary Figure 1 Shortening of tentacles is the first indicator of a polyp’s compromised health.

A healthy Hydra polyp (left) typically has long tentacles armed with nematocytes and displays conspicuous periodically recurring contractions of the body followed by a passive re-extension. Sick polyps (right) can be recognized by shortened tentacles with rounded knobs at their ends (arrowheads). Diseased polyps stay in a permanently elongated or contracted state. Irregular or inappropriate washing of the culture after feeding or exposure to some toxic chemicals from the plastic are main causes for emergence of such diseased polyps.

Supplementary information

Supplementary information

Supplementary Figure 1

Reporting Summary

Supplementary Video 1

Microinjection of a Hydra embryo. (00:00–00:04) A female polyp with a fertilized egg undergoing the first cleavage. (00:05–00:17) Microinjection setup ready for injection. The tips of both the holding capillary (left) and the injection needle (right) are submerged in the injection chamber. (00:18–00:33) The embryo is fixed by the holding capillary (left) and pierced with the injection needle (right) to deliver the transgene into it. (00:34–00:40) The injected embryo continues its development and undergoes a series of cleavages. (00:41–00:43) Three transgenic embryos at the cuticle stage with clearly visible eGFP fluorescence. (00:44–00:51) Transgenic polyp hatches from one of the embryos.

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Klimovich, A., Wittlieb, J. & Bosch, T.C.G. Transgenesis in Hydra to characterize gene function and visualize cell behavior. Nat Protoc 14, 2069–2090 (2019). https://doi.org/10.1038/s41596-019-0173-3

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