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Microfabricated blood vessels for modeling the vascular transport barrier

Nature Protocols (2019) | Download Citation

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Abstract

The vascular endothelium forms the inner lining of blood vessels and actively regulates vascular permeability in response to chemical and physical stimuli. Understanding the molecular pathways and mechanisms that regulate the permeability of blood vessels is of critical importance for developing therapies for cardiovascular dysfunction and disease. Recently, we developed a novel microfluidic human engineered microvessel (hEMV) platform to enable controlled blood flow through a human endothelial lumen within a physiologic 3D extracellular matrix (ECM) into which pericytes and other stromal cells can be introduced to recapitulate tissue-specific microvascular physiology. This protocol describes how to design and fabricate the silicon hEMV device master molds (takes ~1 week) and elastomeric substrates (takes 3 d); how to seed, culture, and apply calibrated fluid shear stress to hEMVs (takes 1–7 d); and how to assess vascular barrier function (takes 1 d) and perform immunofluorescence imaging (takes 3 d).

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.

Code availability

The custom code used for the current study is provided in the Supplementary Data.

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Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Key references using this protocol

Polacheck, W. J. et al. Nature 552, 258–262 (2017): https://doi.org/10.1038/nature24998

Alimperti, S. et al. Proc. Natl. Acad. Sci. USA 114, 8758–8763 (2017): https://doi.org/10.1073/pnas.1618333114

McCurley, A. et al. J. Am. Soc. Nephrol. 28, 1741–1752 (2017): https://doi.org/10.1681/ASN.2016020200

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Acknowledgements

This work was supported in part by grants from the National Institutes of Health (EB00262, EB08396, UH3EB017103, and HL115553) and the National Science Foundation Center for Engineering MechanoBiology (CMMI15-48571). W.J.P. acknowledges support from a Ruth L. Kirchstein National Research Service Award (F32 HL129733) and from the NIH through the Organ Design and Engineering Training program (T32 EB16652); M.L.K. acknowledges support from the Hartwell Foundation and from the National Institutes of Health (K99-CA226366-01A1); and J.B.T. acknowledges support from the NIH through the Translational Research in Biomaterials Training Program (T32 EB006359).

Author information

Author notes

    • William J. Polacheck

    Present address: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, USA

Affiliations

  1. The Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA

    • William J. Polacheck
    • , Matthew L. Kutys
    • , Juliann B. Tefft
    •  & Christopher S. Chen
  2. The Biological Design Center and Department of Biomedical Engineering, Boston University, Boston, MA, USA

    • William J. Polacheck
    • , Matthew L. Kutys
    • , Juliann B. Tefft
    •  & Christopher S. Chen

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Contributions

W.J.P. and C.S.C. conceived of the platform. W.J.P. designed and fabricated the device masters, optimized endothelial cell culture and flow protocols, and developed the permeability assay. W.J.P. and M.L.K. developed the staining and imaging protocols, and M.L.K. and J.B.T. developed the protocols for using hydrogels other than collagen I. J.B.T. optimized the use of pericytes. W.J.P., M.L.K., and J.B.T. performed the experiments and analyzed the data. W.J.P., M.L.K., J.B.T., and C.S.C. wrote the manuscript.

Competing interests

The authors declare no competing interests.

Corresponding author

Correspondence to Christopher S. Chen.

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DOI

https://doi.org/10.1038/s41596-019-0144-8

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