Supplementary Figure 1: Mature eight-week-old pea plants grown in limited media and nutrition (“flask method”) in order to achieve rapid generation advancement. | Nature Protocols

Supplementary Figure 1: Mature eight-week-old pea plants grown in limited media and nutrition (“flask method”) in order to achieve rapid generation advancement.

From: Speed breeding in growth chambers and glasshouses for crop breeding and model plant research

Supplementary Figure 1

Pisum sativum (a) accession JI 2822 and (b) cv. Frisson. Dry seeds were sterilised in 10% sodium hypochlorite, rinsed in sterile water, chipped and left to germinate in the dark for 3 days on sterile, wet filter paper. Germinating seeds were transferred to flasks containing 250 mL fine perlite and silver sand (mixed 50:50) and FP nutrient media which had been sterilised (composition described in Supplementary Table 49). Flasks were placed in the dark for a further 5 days. The seedlings were inoculated with Rhizobium, and the elongated shoot passed through the neck of the flask and held in place with a bung. The base of the flask was covered with a black plastic bag. Plants were grown in a Controlled Environment Room at constant 22 °C with a 16-hour photoperiod. After 3 weeks, flasks were watered with 50 mL FP media once a week. After 8 weeks post germination, plants had mature dry seed ready to harvest as shown (indicated by red arrows). JI 2822 plants grown in the glasshouse under lights required 12 weeks post sowing before mature dry seed were ready for harvest.

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