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A look into the dynamic process of proofreading in DNA polymerase-γ at near-atomic resolution

The correction of errors during mitochondrial DNA replication by the proofreading function of DNA polymerase-γ is crucial for maintaining the integrity of the mitochondrial genome and the production of cellular energy. Using cryo-electron microscopy, several steps of the DNA polymerase-γ proofreading pathway have been revealed at near-atomic resolution.

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Fig. 1: Proposed proofreading pathway in Polγ.


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This is a summary of: Park, J. et al. Polγ coordinates DNA synthesis and proofreading to ensure mitochondrial genome integrity. Nat. Struct. Mol. Biol. (2023).

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A look into the dynamic process of proofreading in DNA polymerase-γ at near-atomic resolution. Nat Struct Mol Biol 30, 722–723 (2023).

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